查询词典 antibody

To use phage display construction antibody library, trabant peptide library, protein mutant library and cDNA library, thus generally using this technology to screen single chain antibody, le...

利用噬菌体表面展示技术可以构建抗体库、随机肽库、蛋白质突变体库和cDNA文库，从而广泛用于单链抗体的筛选、先导化合物的筛选、疫苗的研制等，尤其是在单链抗体、模拟抗原表位的筛选及建立食品安全快速检测体系方面，具有广阔的应用前景。

Objective To discuss the assay methods and clinical value of IgG antibody to Mycobacterium Tuberculosis. Methods To detect the antibody levels of tuberculoprotein 16KDa, 38KDa and LAM by Protein Chip and ELISA together, the sensitivity, specificity and total coincidence rate of the two methods were compared.

目的 探讨结核分枝杆菌IgG抗体的检测及其临床应用价值方法应用蛋白芯片识别仪分析结核病人血清中结核菌蛋白16Kda和38Kda以及脂阿拉伯甘露糖抗体含量，同时与ELISA法进行比较，判断其敏感性、特异性和总符合率。

Methods: Mononuclear cells in umbilical blood and peripheral blood in normal pregnant women and unpregnant women were labled by erythrocyte specific antibody CD71 and fetal specific antibody HbF. Furthermore, NRBC were sorted by flow cytometry.

选用红细胞特异性抗体CD71、胎儿特异性抗体HbF对正常孕妇外周血、未孕妇女和胎儿脐血单个核细胞进行标记，并结合流式细胞仪对孕妇外周血中胎儿有核红细胞进行分选。

To attain more comprehensive understanding of flounder immune system, it is necessary to characterise and localise lymphoid cells within the different lymphoid organs. Use polyclonal antibody or monoclonal antibody as a tool will benefit this kind of study.

制备针对免疫球蛋白的多克隆或单克隆抗体，作为研究工具，对免疫器官中产生抗体的组织和细胞进行定位，可以了解免疫器官在防御疾病过程中是如何发挥作用的，以便对应用疫苗奠定理论基础。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

The affinity constant of the anti-SQ polyclonal antibody 1405~# was 1.22×10~8L/mol. The cross reactivity with other SAs was less than 0.17%. Three hybridoms cell lines secreting monoclonal antibody against SQ were obtained.

同时，以SQ-BSA为免疫原免疫Balb／c小鼠，经融合实验获得了三株分泌抗磺胺喹噁啉单克隆抗体的杂交瘤细胞株SQl_(12)A_(10)，SQ_(26)B_7，SQ_(28)H_7。

Antibody level of group A was higher than that of group C in all-aged population. Antibody of group A ECM of children in kindergarten and adults were lower than the others while that of group C decreased slightly with aged.

不同年龄段人群的A群抗体水平均高于C群，幼儿园幼儿和成年人 A群抗体水平低于其他年龄段，C群抗体水平随年龄的增加逐渐降低。

Check that secondary antibody is binding specifically. Run a secondary control without primary antibody.

请检查您使用的二抗是否具有专一性：使用没有一抗的二抗对照组作检查。

Here we report that there is a dramatic decrease in the ability of a broadly neutralizing antibody to protect macaques against SHIV challenge when Fc receptor and complement-binding activities are engineered out of the antibody.

但一项新的研究表明，抗HIV抗体在以两种方式发挥作用时最有效：一种是通过中和，即直接杀死病毒，阻止其进入T-细胞；另一种是通过杀死感染的细胞。

The immunoglobulin like anti AChR antibody without complement binding activity is successfully made from a pathogenic anti AChR antibody IgG 637 by site directed mutagenesis.Key words:receptor, cholinergic;myasthenia gravis;point mutation;complement-binding;antibodies

本研究利用定点突变技术，从完型抗乙酰胆碱受体抗体IgG 637制备了不能激活补体的突变型抗乙酰胆碱受体抗体IgG 637/K 322 A，为最终制备出可用于治疗重症肌无力的无补体激活功能的单价抗乙酰胆碱受体抗体提供依据。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。