查询词典 antibody

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Results We found GM1 antibody in 36% and cephalin antibody in 43% of MS patients in serum,GM1 antibody in 11% and cephalin antibody 18% in cerebrospinal fluid.

为了探讨脂质抗体在MS中的作用，检测了MS患者血清和脑脊液中的GM1抗体、脑磷脂抗体和髓鞘碱性蛋白，意欲进一步揭示MS的免疫机制。

Results We found GM1 antibody in 36% and cephalin antibody in 43% of MS patients in serum,GM1 antibody in 11% and cephalin antibody 18% in cerebrospinal fluid.The levels of antibody in serum of treated_MS patients were significantly lower than those in active MS patients.The levels of myelin basic protein in serum and cerebrospinal fluid in MS were significantly higher than those in control group.

结果 多发性硬化患者血清GM1抗体阳性率 36%，脑磷脂抗体为43%；脑脊液GM1抗体为11%，脑磷脂抗体为18%，与对照组比较差异均有显著性；血清和脑脊液的髓鞘碱性蛋白增高亦有意义。

Results We found GM1 antibody in 36%and cephalin antibody in 43%of MS patients in serum,GM1 antibody in 11%and cephalin antibody 18%in cerebrospinal fluid.The levels of antibody in serum of treated_MS patients were significantly lower than those in active MS patients.

结果 多发性硬化患者血清GM1抗体阳性率为36%，脑磷脂抗体为43%；脑脊液GM1抗体为11%，脑磷脂抗体为18%，与对照组比较差异均有显著性；血清和脑脊液的髓鞘碱性蛋白增高亦有意义。

Culture and laboratory tests, including fluorescence-labeled antibody staining, PCR of virulence factor genes and Ascoli precipitin test on tissue specimens, identified the organism as Bacillus anthracis.

组织检体进行细菌培养及其他实验室检查，包括FA染色(fluorescence-labeled antibody staining)、毒力因子基因PCR及Ascoli precipitin test，证实病原为炭疽杆菌。

ELISA showed that antibody responses induced by propolis vaccine were faster than that of oil-emulsion vaccines and aluminum hydroxide vaccines;antibody responses induced by oil-emulsion vaccines were longer in duration than that of propolis vaccines and aluminum hydroxide vaccines;the maximum antibody responses induced by oil-emulsion vaccines and propolis vaccines were higher than that of aluminum hydroxide vaccines;Antibody levels were all significantly boosted by revaccination of these vaccines and in addition to this,oil-emulsion vaccines showed longer antibody duration.

这七种疫苗在抗体产生速度方面以蜂胶苗较快，而油剂苗和铝胶苗免疫后抗体上升速度较慢；在抗体水平的维持能力方面，油剂苗较蜂胶苗和铝胶苗更强；在产生最高抗体水平方面，油剂苗和蜂胶苗能力相当且较铝胶苗为高；在加强免疫的效果方面，这些疫苗二次免疫所产生的抗体水平均较一次免疫更高，蜂胶苗和铝胶苗在第93天时的二免抗体水平已降至同一免相当，但此时油剂苗有着较一免更高的二免抗体水平。

OBJECTIVE To investigate the effects of extracorporeal circulation on anticardiolipin antibody before and after operation and its clinical significance.

目的探讨体外循环对抗心磷脂抗体(anticardiolipin antibody ACL-Ab)的影响及其意义。

There is still high incidence of hepatitis A in the developping country. The wide-spread use of HAV live attenuated vaccine and inactive vaccine has provided efficient ways for controlling the epidemic of heptatitis A. while, some people got hepatitis A after injecting vaccines. In order to distinguish the viral capsid could result from hepatitis A vaccines or from natural infection, the identification of the virus strains is necessary. In 1975, KO|¨hler and Milstein have developped hybridoma technique for producting monoclonal antibody.

甲型肝炎在发展中国家多年来都有不同程度的流行，甲肝减毒活疫苗和灭活疫苗的使用，为甲肝的预防提供了可靠的手段，但偶有在接种疫苗后发生甲型肝炎的案例，这就需要对患者所感染的病原体进行鉴别，以明确患者发病的原因是接种疫苗引发还是甲肝野毒株感染导致的偶合病例。1975年KOhler和Milstein建立了杂交瘤技术制备了单克隆抗体(monoclonal antibody；McAb)。

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原，注射新西兰白兔制备出效价高达1：32000的多克隆抗体作为第一抗体，第二抗体为辣根过氧化物酶标记的羊抗兔IgG，通过交叉稀释法确定抗原、一抗及二抗的最优反应条件，建立酶联免疫吸附分析方法体系，病毒检测灵敏度达30ng。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。