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Anti-CCP1 and anti-CCP2 antibody tests were performed on 79 patients with arthropathy. The sensitivity, specificity, positive predictive value, and negative predictive value for-discriminating between rheumatoid arthritis and non-RA were calculated for both tests.

材料与方法:79名关节病变的患者同时以Anti-CCP1 和anti-CCP2 抗体做测试,并区别类风湿性关节炎与非类风湿性关节炎的敏感度、特异性、阳性预测值和阴性预测值。

Phenotype analysis of transgenic mice Anti-IgM/anti-IgD antibodies, anti-IgM/anti-IgM〓/anti-IgM〓 antibodies were used to analyze allelic exclusion of transgenic mice B cells, and it was shown that 50%~80% B cells express transgene encoded IgM. ELISA was used to evaluate serum antikeratin IgM and total IgM of transgenic mice peripheral blood, and it is found that sera antikeratin IgM level of several lines of transgenic mice is significantly higher than littermate control. However, there is no difference in serum total IgM level between transgenic mice and non-transgenic mice.

三、抗体重链转基因小鼠表型分析以anti-IgM/anti-IgD抗体、anti-IgM/anti-IgM〓/anti-IgM〓抗体分析转基因小鼠B细胞等位基因排斥情况,发现50%~80%的B细胞表达转基因编码的IgM;ELISA分析转基因小鼠外周血中抗角蛋白IgM和总IgM水平,发现部分转基因小鼠的抗角蛋白IgM水平明显高于对照小鼠,而总IgM水平没有差异。

Anti-B220/anti-IgM antibodies were used to analyze number and maturation of central and peripheral B cells in transgenic mice. It is found that number of mature B cells decreased in bone marrow, spleen, peritoneal cavity. B cells in bone marrow of Tg mice were just one half of that of control, and in spleen, this number was even one third of that of the control.

以anti-B220/anti-IgM抗体分析了转基因小鼠中枢和外周B细胞数的变化及成熟情况,发现骨髓、脾和腹腔的成熟B细胞数都明显下降,其中骨髓和腹腔约为对照野生鼠的一半,而脾更低至三分之一。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

The minimal ventricular rates ranged from 46 to 80 beats per minute. VVJ mode pacemakers were inserted through xyphoid approach in all live-birth infants. Complications were noted in three of them (50%). Antinuclear antibody and anti-SSA/Ro antibody were positive in all 8 mothers (100%). The anti-SSB/La antibody was positive in 6 of the eight mothers (75%). Five infants tested positive for anti-SSA/Ro antibody.

所有活产婴孩均经由剑突下途迳植入心律调节器,3个(50%)有并发症。8个母亲(100%)有阳性抗核抗体及anti-SSA/Ro抗体,6个母亲有阳性anti-SSB/La抗体。5个婴孩有阳性anti-SSA/Ro抗体,所有婴孩均没有anti-SSB/La抗体。

Third,international community has already proposed anti-monopoly law, we can make reference to European Anti-competition law,through case laws and mechanism,e.g.how to judge the monopoly behavious of corporations in the market,how to determine the corporation\'s leading position in the market,to monopolize the market through merger and acquisition of other corporations;besides,we can also make reference to law of other countries such as Fair Trading Act of the U.K.,the Competition Act of the U.K.,the Guidelines of merger in the U.S., Antitrust Act of the U.S.,the Guidelines of European Anti-trust laws, Guidelines on merger in European Union,and the Trade-Related Anti-Trust Measures,the Trade Related Investment Measures and etc.

第三,国际社会提出制定反垄断法的模式,我们可以参考欧盟垄断法,通过案例和机制,例如:怎样判定企业在市场的垄断行为、怎样界定市场支配地位、对公司通过合并与收购的行为来把市场垄断;再且,我们也会参考一些其它国家如英国公平贸易法、英国竞争法、美国的合并指引、美国垄断法、欧盟垄断法规指引、欧盟合并条例,还有《与贸易有关的及垄断措施协议》(Trade-Related Anti-Trust Measurers)、《与贸易有关的投资措施》(trade Related Investment Measures)等法规、条例来参考讨论。

In addition, CRT has anti-glare surface, anti-static the AGAS (Anti-GlareAnti-Static) coating, anti-reflective, anti-static ARAS (Anti-ReflectionAnti-Static) coating, hand touch, but also in the top left under the hand, do not believe you see the display from the side, you can see a handprint on your screen, do you want to help the Public Security Bureau's busy uncle in advance display extracted harm "murderer" of the fingerprint it?

另外,CRT的表面有防强光、防静电的AGAS(Anti-GlareAnti-Static)涂层,防反射、防静电的ARAS(Anti-ReflectionAnti-Static)涂层,用手触摸,还会在上面留下手印,不信你从侧面看显示器,就能看到一个个手印在你的屏幕上,难道你想帮公安局叔叔们的忙,提前提取出伤害显示器&凶手&的指纹吗?

In addition, CRT's surface anti-glare, anti-static the AGAS (Anti-GlareAnti-Static) coating, anti-reflective, anti-static of the ARAS (Anti-ReflectionAnti-Static) coating, hand-touch, but also to stay above The fingerprint, do not believe you can display from the side, you can see one in your handprint on the screen, do you want to help the public security bureau uncle's busy, in advance extract hurt Monitors "murderer" fingerprint?

另外,CRT的表面有防强光、防静电的AGAS(Anti-GlareAnti-Static)涂层,防反射、防静电的ARAS(Anti-ReflectionAnti-Static)涂层,用手触摸,还会在上面留下手印,不信你从侧面看显示器,就能看到一个个手印在你的屏幕上,难道你想帮公安局叔叔们的忙,提前提取出伤害显示器&凶手&的指纹吗?

For example, oil, and now the general of the CRT screen surface coated with anti-glare, anti-static the AGAS (Anti-GlareAnti-Static) coating, anti-reflective, anti-static ARAS (Anti-ReflectionAnti-Static) coating, and your hands will destroy the oil coating the surface of the display.

比如说油脂,现在的CRT屏幕表面一般涂有防强光、防静电的AGAS(Anti-GlareAnti-Static)涂层,防反射、防静电的ARAS(Anti-ReflectionAnti-Static)涂层,而你手上的油脂会破坏显示器表面的涂层。

KAKA Anti-dry Spray An anti-skin to be used with all offset inks FEATURES AND BENEFITS: l Can apply to the ink in open ink cans, to prevent skin from forming in the ink cans during storage of the ink l Gains long-lasting protection with all litho and letter press inks l Low to no odour when applied to the ink rollers l Will not affect the ink or the color of the ink l Longer press stops for color adjustments APPLICATION SCOPES: Anti-dry can also be applied to the ink in open ink cans,to prevent skin from forming in the ink cans during storage of the ink.

中文备注: KAKA 油墨防干剂(Anti-dry Spray) KAKA油墨防干剂是一种在印刷过程中临时停机,为防止油墨与空气产生氧化喷射于油墨表面,避免油墨由于干燥做成结皮、起光面,影响油墨的流动性直接阻碍印刷质量的控制。,另外对未用完的罐中油墨将防干剂喷射于油墨表面上可起到更长保存时间,减少不必要的资源浪费。适用于墨槽上的油墨表面与平版及凸版印刷上的油墨得到长时间的保护。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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