查询词典 anti-epithelial Serum

Moreover, in some SLE patients serum level of BAFF is elevated and correlated with serum anti-dsDNA titer.

部分系统性红斑狼疮患者中BAFF的水平有不同程度增高，并与抗dsDNA抗体滴度相关。

To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le phenotype cells, patient phenotype was identified by using anti-Le and anti-Le blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly.

为了分析1例溶血性输血反应产生的原因，用谱细胞和Le表型细胞鉴定患者血清中的抗体，用抗Le和抗Le血型试剂鉴定患者红细胞表型，用PCR测序方法分析LE基因（FUT3基因）全编码区序列。

objective to study the anti-tumor activity and enhancing immune effect of phellinus linteus.methods the anti-tumor activity of phellinus linteus was estimated by the longevity of eac mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the il-2 concentration in serum of tumorous mice was determined by elisa.results phellinus linteus can prolong eac mouse's life and can increase the il-2 concentration in serum of tumorous mouse but can't affect the immune effect of tumorous mouse.conclusion chinese hurb phellinus linteus possess some antitumor power for tumor-loaded mice,but further research is needed.

目的 观察桑黄对肿瘤的抑制作用及对机体免疫功能的影响。方法以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用，以炭末廓清实验观察荷瘤小鼠非特异性免疫功能，以elisa法测定荷瘤小鼠血清il-2浓度。结果桑黄对eac荷瘤小鼠的生存时间有延长作用，对荷瘤小鼠血清il-2浓度有提高作用，对荷瘤小鼠非特异性免疫功能无明显影响。结论中药桑黄对荷瘤小鼠具有一定的抗肿瘤能力，作用的强度和作用机制的深度还有待进一步研究。

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集：正常人的血清：采集沈阳市正常献血员外周静脉血共215份，待血凝后离心分离血清；患者的血清：来自中国医科大学一院及吉林大学二院门诊及住院部的病人，梅毒患者除了病史及临床表现外，全部经血清学TPHA及RPR确认；寻常性天疱疮患者除了临床及组织病理诊断外，还进行了直间接免疫荧光的检测，直接免诊断疫荧光：在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积；间接免疫荧光用鼠或兔的舌或食管作为底物，在病人的血清中显示有肯定的细胞间抗体。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The serum levels of TChE and SOD and CAT in hippocampus of mice in Cold group was also significantly higher than those in the Control group and Warm group(P 0.05或P 0.01),while the serum level of MDA in the Control group and Warm group were higher than that in the Cold group(P 0.05或P 0.01). Conclusion:(1)Long proper winter swimming could improve learning and memory abilities of mice.(2) Winter swimming could improve the ability of learning and memory by coordinate the content of acetylcholinesterase (3) This effect may be related to the decreased activity of TChE and the increased activity of anti-oxidatio.

1长期适量的冬泳运动可以促进小鼠学习记忆能力。2冬泳运动可以通过增加小鼠脑内海马组织中ACh的含量而影响TChE的活力。3冬泳运动促进学习记忆的机制可能是：3.1冬泳运动通过冷应激引起的神经内分泌系统的反应，来改变中枢神经系统的功能，使小鼠脑内乙酰胆碱的代谢增强、含量增加，从而促进学习记忆能力。3.2冬泳运动通过增强机体清除自由基的能力，来减轻脂质过氧化的程度，对小鼠的脑损伤起到保护作用，从而促进学习记忆能力。

Aim To develop a sandwich ELISA for measuring serum β2-glycoprotein I-lipoprotein complex (β2-GPI-Lp concentration, and to explore the existence of serum complexes β2-GPI-Lp and its clinical value for IgA nephropathy. Methods ELISA was established using rabbit anti-human β2-GPI antibody as the capture antibody, and quantitating with polyclonal antibody against apolipoprotein enzyme conjugate.

目的 建立检测人血清β2-糖蛋白I-脂蛋白复合物的酶联免疫吸附法，探讨该复合物存在于人血循环中的可能性，并通过对IgA肾病患者的检测分析该复合物的临床诊断价值。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

Objective To discuss the value of detection of serum serum anti-nuotrophil cytoplasmic autoantibodies and anti-vascular endothelial cell antibodies in the diagnosis of erythema nodosum. Methods Samples from erythema nodosum patients and normal people were collected, the ANCA and AECA were detected by indirect immunofluorescence.

目的 探讨血清抗中性粒细胞胞浆抗体及抗血管内皮细胞抗体在结节性红斑发病中的作用和意义方法收集结节性红斑患者和正常人血清，用间接免疫荧光技术检测血清中ANCA和AECA，并分型。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。