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The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤,对其中10株进行克隆化,获得bFGF特异单抗16株,2株针对融合蛋白;应用该单抗建立了0.1ng/孔灵敏度的ELISA,0.5ng/孔敏度的斑点ELISA;用Western-blotting鉴别了经改造不同工程菌蛋白表达,指导重组工程菌改造;用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点;合作研究了20例肝癌、肝癌细胞株和正常肝组织,发现前者bFGF高表达;应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF,纯度达到95%;发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体,已有10多家单位引用单抗或进行合作。

It has shown by the studies at home and abroad that Chaga is the ideal companion which has health care, prevention function and can treated many diseases. The healthy groups can use it for fitness beauty、disease prevention and health care; sub-health groups can restore nature health; and disease groups regards its as the best natural therapeutic products without any side effects. The Chaga's own characteristics determines its using value in the health care market, which can be taken as the health products, that is, regulating immunity, anti-virus, anti-cancer, balance blood sugar, balancing blood pressure, improving cardiac function, treating various incurable diseases, removing free radicals, anti-oxidation, anti-aging, and so on.

国内外研究证明桦褐菌是21世纪人类最理想的保健、预防、治疗多种疾病的最好伴侣,健康的群体可以用之健身美容、防病保健,亚健康群体靠其还原健康的本色,疾病群体用其作为最好的天然、无任何副作用的食疗制剂,桦褐菌自身的特点决定了其在健康产品市场的应用价值,可作为调节免疫、抗病毒、抗癌、平衡血糖、平衡血压、改善心功能、治疗各种疑难杂症、清除体内自由基、抗氧化、延缓衰老等健康产品。

This paper through taking European Association as a representative of the international anti-dumping policy's evolvement and the discussion of the accountancy standard to the market economy position cognizance. Put forward establishment the international anti-dumping evadable accounting, establishment the international anti-dumping litigation accounting, According to the international usual practice to carry on independent audit, Speed the development of international anti-dumping accountancy's talented person, this four accountancy's measures.

本文通过对以欧盟为代表的国际反倾销政策演变及其对市场经济地位认定的会计标准的探讨,提出了建立国际反倾销规避会计,建立国际反倾销应诉会计,按国际惯例进行独立审计,加快国际反倾销会计人才的培养四条会计措施。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Researches have shown that Mytilus coruscus extract has functions such as anti-tumor, anti-aging, anti-viral and anti-bacterial, enhancing immunity and lowering blood lipid content.

已有研究表明,贻贝提取物有抗肿瘤、抗衰老、抗病毒、抗酒精性肝损伤和抗菌的功能和增加机体免疫能力、降低血脂含量的功效。

Innovativeink anti-false more than the production materials, with practicalbility anti-false anti-false, performant, the fastest growing packaging business card printing and membership card making anti-false high-tech world.

油墨不攻假比承印质料不攻假更具有实用性,不攻假本能机能更不弱,成为纲前成长最迟缓的包装制卡和会员卡制作不攻假矮陈技艺之一。

The results of the ability of anti-oxidation scavenging DPPH free radical shows that polyhydroxy substituted N-benzoylserotonin derivatives (compound 1-10) have the generally better ability of anti-oxidation than control group, in which the IC50 of compound 1-10 ranges 8.75 to 18.02 μg/ml and the IC50 of trolox is 13.04 μg/ml. The abilities of anti-oxidation of compound 1, 4, 5, 7, and 10 are all better than the control group, trolox. Among all, compound 10 has the best ability of anti-oxidation (IC50 is 8.75 μg/ml). This study also discovers that the ability of scavenging DPPH free radical is strongly related to the number and the position of hydroxyl.

在清除DPPH自由基的抗氧化能力结果显示,相较於对照组水溶性维生素E之IC50值13.04 μg/ml,多羟基取代之N-色洛冬宁苯甲醯胺衍生物(化合物 1 ~ 10),皆具有相当不错的抗氧化能力,十种醯胺化合物的 IC50值介於8.75 ~ 18.02 μg/ml之间,化合物1、4、5、7、10的抗氧化能力效果皆优於对照组水溶性维生素E的IC50值,其中又以化合物10的效果最佳(IC50值8.75 μg/ml),而清除DPPH自由基的能力发现与羟基的数量及其位置有关。

The section include how to prevent the anti-dumping and how to counter the anti-dumping, analysed the countermesure of anti-dumping, I point out we can subduce the anti-dumping by actively counter, lighten the influence on the economic.

第二节从如何防止反倾销和如何应对反倾销两个方面具体阐述了我国企业应对反倾销的对策,通过分析笔者指出通过积极的应对,我国完全可以将国外对我国进行的反倾销行为降到最少,大大减轻对我国经济的影响。

Note: A Christian who is a Pietist, who seeks an intimate relationship with God, does not necessarily has to be anti-intellectual, anti-rational, anti-culture, and anti-theological.

注﹕敬虔主,追求与神亲密交通,不一定等于反知识,反理性,反文化,反神学!

Anticorrosion engineering: high corrosion and rust engineering, vessel wall aggrandizement anticorrosive chimney brush color ring, cold water tower corrosion, jia steel structure antisepsis, furnace frame anticorrosive, frame anticorrosive, chimney, iron pipe anticorrosion paint anticorrosion, urea prilling tower, cooling tower corrosion and corrosion poison, radio and television tower corrosion, microwave towers, anti-corrosion, anti-corrosion chimney (packet transmission clamp, platform, anti-corrosion, nets) ladder, anti-corrosive, corrosion and corrosion, drmg-crmg road building exterior brush paint, hotel, coal conveying tents, crane bridge anticorrosion construction corrosion.

防腐工程:高空防腐除锈工程、罐体内外壁强化防腐烟囱刷色环航标,凉水塔防腐,钢结构防腐,炉架防腐,网架防腐、管道防腐,铁烟囱油漆防腐,尿素造粒塔、排毒塔防腐,冷却塔防腐,广播电视塔防腐,微波塔防腐,发射塔防腐,烟囱(包箍、平台、爬梯、护网)防腐,储油罐防腐,龙门吊防腐、行车防腐,宾馆、大厦外墙刷涂料,煤棚输煤栈桥防腐,吊机防腐等施工工程。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力