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①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, And then back to your hotel

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯是古王国的首都和最重要的城市之一整个古埃及，午餐休息时间，历史，然后我们开车到吉萨访问吉萨大金字塔和神秘的狮身人面像，然后返回旅馆旅游包括：1。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, And then back to your hotel and over night in your hotel in cairo Day 3A day's sightseeing to the Egyptian museum, which features artifacts from the pharaonic period.

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯是古王国的首都和最重要的城市之一整个古埃及，午餐休息时间，历史，然后我们开车到吉萨访问吉萨大金字塔和神秘的狮身人面像，然后返回旅馆过夜，并在您的酒店在开罗第3天一天的观光的埃及博物馆，其特点从法老时期文物。

Sakkara has the first pyramid and the world's oldest stone structure,later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, back to your hotel and over night in your hotel in CairoDay 3 After breakfast, you will start your tour to visit the Egyptian museum which contains the fabulous treasures of Tut-Ankamon specially the solid golden mosque and the golden coffer then proceed to the old city of Cairo to visit the hanging church.

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯是古王国的首都和最重要的城市之一整个古埃及，午餐休息时间，历史，然后我们开车到吉萨访问吉萨大金字塔和神秘的狮身人面像，回到旅馆，并连夜在您的酒店在开罗第3天早餐后，您就可以开始您的游览参观埃及博物馆，它包含了图坦卡蒙的神话般的财富，特别是固体Ankamon黄金清真寺和黄金库房，然后着手开罗老城区参观挂教堂。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic Sphinx, back to your hotel and over night in your hotel in Cairo Day 3 After breakfast, you will start your tour to visit the Egyptian museum which contains the fabulous treasures of Tut-Ankamon specially the solid golden mosque and the golden coffer then proceed to the old city of Cairo to visit the hanging church.

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯是古王国的首都和最重要的城市之一整个古埃及，午餐休息时间，历史，然后我们开车到吉萨访问吉萨大金字塔和神秘的狮身人面像，回到旅馆和你在开罗举行第3天晚上在酒店早餐后，您就可以开始您的游览参观埃及博物馆，它包含了图坦卡蒙的神话般的财富，特别是固体Ankamon金清真寺和金库房，然后着手开罗老城区参观挂教堂。

Sakkara has the first pyramid and the world,s oldest stone structure,later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch,and then we drive to Giza to visit the great Pyramids of Giza and the enigmatic Sphinx, back to your hotel and over night in your hotel in CairoDay 3 After breakfast, you will start your tour to visit the Egyptian museum which contains the fabulous treasures of Tut-Ankamon specially the solid golden mosque and the golden coffer then proceed to the old city of Cairo to visit the hanging chuch .

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯是资本古代王国和一个最重要的城市在整个历史的古埃及，休息，午餐，然后我们开车到吉萨参观了吉萨大金字塔和神秘的狮身人面像，回到旅馆，并连夜在您的酒店在开罗第3天早餐后，您就可以开始您的游览参观埃及博物馆，其中载有神话般的财富的图坦卡蒙，Ankamon特别坚实的金色清真寺和黄金库房然后进行旧城开罗访问挂chuch。

Sakkara has the first pyramid and the world's oldest stone structure,later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, back to your hotel and over night in your hotel in Cairo Day 3 After breakfast, you will start your tour to visit the Egyptian museum which contains the fabulous treasures of Tut-Ankamon specially the solid golden mosque and the golden coffer then proceed to the old city of Cairo to visit the hanging church.

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯，是资本的古代王国，一个最重要的城市，在整个历史的古埃及，休息，午餐，然后我们开车到吉萨访问伟大的吉萨金字塔和神秘的狮身人面像，回到旅馆，并连夜在您的酒店在开罗第3天早餐后，您就可以开始您的游览参观埃及博物馆，其中载有神话般的财富的图坦卡蒙，Ankamon特别坚实的金色清真寺和黄金库房然后进行旧城开罗访问挂教堂。

Sakkara has the first pyramid and the world's oldest stone structure,later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, back to your hotel and over night in your hotel in Cairo Day 3 After breakfast, you will start your tour to visit the Egyptian museum which contains the fabulous treasures of Tut-Ankamon specially the solid golden mosque and the golden coffer then proceed to the old city of Cairo to visit the hanging church.

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯是古王国的首都和最重要的城市之一整个古埃及，午餐休息时间，历史，然后我们开车到吉萨访问吉萨大金字塔和神秘的狮身人面像，回到旅馆，并连夜在您的酒店在开罗第3天早餐后，您就可以开始您的游览参观埃及博物馆，它包含了图坦卡蒙的神话般的财富，特别是固体Ankamon黄金清真寺和黄金库房，然后着手开罗老城区参观挂教堂。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic Sphinx, back to your hotel and over night in your hotel in Cairo Day 3 After breakfast, you will start your tour to visit the Egyptian museum which contains the fabulous treasures of Tut-Ankamon specially the solid golden mosque and the golden coffer then proceed to the old city of Cairo to visit the hanging church.

塞加拉金字塔的第一个和世界最古老的石结构，后来开车到孟菲斯，是资本的古代王国，一个最重要的城市，在整个历史的古埃及，休息，午餐，然后我们开车到吉萨访问伟大的吉萨金字塔和神秘的狮身人面像，回到旅馆，并连夜在您的酒店在开罗举行第3天早餐后，您就可以开始您的游览参观埃及博物馆，其中载有神话般的财富的图坦卡蒙，Ankamon特别固体金清真寺和金库房然后进行旧城开罗访问挂教堂。

Sakkara has the first pyramid and the world's oldest stone structure, later we drive to Memphis is the capital of the ancient kingdom and one of the most important cities throughout the history of ancient Egypt, break for lunch, and then we drive to Giza to visit the great pyramids of Giza and the enigmatic sphinx, before the tour ends, we will leave the car behind and venture forth on a horse or a camel for one hour and half into the ancient history of Giza plateau, enjoying watching the sunset in the pyramids. And then back to your hotel

塞加拉金字塔的第一个和世界上最古老的石结构，驱动器后，我们的首都孟菲斯的古老王国和最重要的一个城市的整个历史的古埃及，打破吃午饭，然后开车到吉萨访问伟大的吉萨金字塔和神秘的狮身人面像，在巡回赛结束后，我们将离开车后面和合资企业提出的关于马或骆驼的一个半小时到古代历史的吉萨高原，享受观看日落的金字塔。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。