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First we give the definition of semicontinuous and subharmonic functions and the propertities of the semicontinuous and subharmonic functions and then introduce the notion of plurisubharmonic functions and the propertities of psh functions, then we give the definition of closed positive currents and the relation between psh functi.

首先总结了半连续函数和次调和函数的定义及其性质，然后在此基础上介绍了多次调和函数的定义及其性质，引入了正闭流的定义，说明了正闭流与多次调和函数之间的联系。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Have no respect and then have no education","trusted and then educated.change former old idea, give kid increment life experience, let the kid is in the feeling growth.but the preachment ising not pure and empty.only in all the environments of the reality, personally experience, work hard feeling, would have to realize.the rain lou seen the sunlight of beauty and moistenned thus, and teacher and parents of hot slice to expect.the concern of science, sincerely of respect, thorough of comprehension, excellent of demonstration, will let our teacher of work become so of great, certainly also let our education change of more meaningful, will also let our student more feeling growth of happiness and beauty.

没有尊重就没有教育"，"没有信任就没有教育。改变以往陈旧的观念，给孩子增加生活体验，让孩子在感受中成长。而不是单纯的、空洞的说教。只有在一切真实的环境中，亲身体验，用心感受，才会有所体会。从而看到美丽的阳光和滋润的雨露，以及老师和家长们的热切期盼。科学的关注，真心的尊重，深入的理解，优秀的示范，会让我们教师的工作变得那么的伟大，当然也会让我们的教育变的更加有意义，也会让我们的学生更加感受成长的快乐和美丽。

The first type is the scanner signature files that provides a Kodak, Agfa or Fuji company's standard quadrochromatic and the quadrochromatic standard data, use of scanner input these quadrochromatic, scan data and the difference between the standard quadrochromatic data reflects the scanner's properties; the second is the display characteristics of the file, which provides software that you can detect your display's color temperature, and then generate a color swatch on the screen, these swatches of information reflects the characteristics of the display; and the third being the print device signature files, it also provides a suite of software, the software in a computer-generated that contains hundreds of color swatch in the graphic, and then the graphic output on the output device, if it is a printer, printing press with respect to direct proof in respect of the first film, proofing and printing, the image on the output measure that is a reflection of the print device's characteristics file information.

第一类是扫描仪特征文件，它供应了柯达、爱克发、富士公司的尺度草稿及这些草稿的尺度数据，诳骗扫描仪不输不入这些草稿，扫描数据与尺度草稿数据的差值反响了扫描仪的共性；第二类是表现器的特征文件，它供应了一些软件，可测不入表现器的色温，然后在屏幕上天生一色块，这些色块新闻反响不入了表现器的特征；第不一类为打印摆设的特征文件，它也供应了一套软件，该软件在计算机洋天生一个含有数百个色块的图形，然后不兵图形在输不入摆设上输不入，如果是打印机不离间接打样，印刷机不离不后不入胶片、打样再印刷，差这些输不入的图像举行丈度即反响不入打印摆设的特征文件新闻。

So saying, with dispatchful looks in haste She turns, on hospitable thoughts intent What choice to chuse for delicacie best, What order, so contriv'd as not to mix Tastes, not well joynd, inelegant, but bring [ 335 ] Taste after taste upheld with kindliest change, Bestirs her then, and from each tender stalk Whatever Earth all-bearing Mother yields In India East or West, or middle shoare In Pontus or the Punic Coast, or where [ 340 ] Alcinous reign'd, fruit of all kindes, in coate, Rough, or smooth rin'd, or bearded husk, or shell She gathers, Tribute large, and on the board Heaps with unsparing hand; for drink the Grape She crushes, inoffensive moust, and meathes [ 345 ] From many a berrie, and from sweet kernels prest She tempers dulcet creams, nor these to hold Wants her fit vessels pure, then strews the ground With Rose and Odours from the shrub unfum'd.

Neerer his presence Adam though not awd， Yet with submiss approach and reverence meek， As to a superior Nature， bowing low， [ 360 ] 她一边说着，一边带着急迫的神情，急速转身，一心想着招待的事，怎样精选最上等最可口的东西，风味若不调和，便会变得粗劣，务必要按照自然的变化而加调味，一样接着一样，有条不紊，引人入胜；于是忙着去采集各种的果品，从大地母亲所生产的万汇中千挑万选，无论东西印度、地中海滨各国、本都、布匿沿岸或阿西诺斯国中的，样样都有，结在柔枝上的，有果皮光滑的，粗糙的，果壳带须的，包在荚里的，她都不吝惜地采摘，堆积在桌子上。

Firstly, the paper introduced the working principle and device of the two-pass grate-rotary kiln and had an analysis method of mechanics of the tyre. We have put forward the finite element analysis of the contact stress of the tyre-roller based on the whole construction mode of shell-kiln liner-large gear-tyre-roller of the rotary kiln and obtained the strength and distributing rule of the tyre's contact stress. The research shows that within one week the contact stress mainly lies in two leading peak value and catastrophe which appear at the contact zone of the tyre and supporting roller. Then we regarded the tyre's stress as the test of unknown plane stress of principal stress. Utilizing mature and modern testing technology, we raised the testing principle and scheme of tyre stress and then had a on-the-spot test of the stress. Comparing the analysis result of the test with the one calculated from the finite element analysis, it proofed the rationality of finite element analysis method of integral modeling and the correctness of the contact stress result of the tyre and roller. Finally, for two-pass of similar rotary kilns, using the ANSYS APDL re-development technology, carried on the re-development about the overall finite element analytic method of thecontact stress of tyre-roller.

首先，本文介绍了2档回转窑的工作原理和装置，对滚圈的力学行为进行了分析，提出了基于筒体-滚圈-托轮的回转窑整体建模的有限元分析法，来对滚圈-托轮的接触应力进行有限元分析与计算，得到了滚圈接触应力的大小和分布规律，并求得滚圈外中内圈节点的主应力；其次，把滚圈的受力看成是主应力未知的平面应力的测试，利用现代测试技术，提出了滚圈主应力测试的原理和方案，并对滚圈的主应力进行了现场测试，现场测试分析结果与有限元分析计算出的主应力相比较，证明了整体法建模的有限元分析方法的合理性和滚圈-托轮接触应力结果的正确性；最后，利用ANSYS的APDL的二次开发技术，针对2档同类回转窑，就滚圈-托轮接触应力的整体有限元分析法进行了二次开发。

Since the number of trips is a non-negative integer, truncated, and of endogenous stratification, this study empirically analyzes its recreational benefits by the On-site Poisson and truncated negative binomial with endogenous stratification of the Travel Cost Method. Besides, this study adopts TNBES to see how the number of trips disperse and takes the consumer surplus to assess benefits. As to visitors' valuation, this research uses the ordered probit to do empirical analysis and then divides all satisfaction factors by the factor analysis and cluster analysis into four factors and two clusters, which then are taken as explanatory variables.

考量旅游次数为非负整数、截断、内生分层等特质，本研究在游憩效益方面，采旅游成本法中的现场波松(On-site Poisson)、截断式内生分层负二项(Truncated Negtive Binomial with Endogenous Stratification， TNBES)模型做实证分析，并於TNBES模型检定旅游次数之扩散情形，效益之评估采消费者剩余为计算标准；在游客评价方面，采次序波比模型做实证分析，并将游客对科博馆各项因素满意度以因素分析、集群分析，区分为四大因素构面与二大集群，纳入游客评价为解释变数，并探讨消费者剩余对游客评价之影响。

Now if you lack any of these tempers, if the whole bent of your heart and mind is not set upon them, all pretences to an immediate inspiration and continual operation of the Spirit of God in your soul are vague and groundless. Keep within the bounds here set you; call nothing a proof of the Spirit or work of God in your soul, but these tempers, and the works which they produce, and then, and not till then, you may safely say with St.

若你现在缺少这些特质、这些性情，心思意念也没有放在这些事情上，心中对圣灵引导这件事，觉得模糊没把握，那麼切切地向上帝求，向祂要这些圣灵的明证，直到上帝的这些性情本质内化成形在你心中，你才能安心地如使徒约翰所说：「并且有圣灵作见证，因为圣灵就是真理。

When I see Him I will fall down in prostration before Him, and He will leave me as long as He will, and then He will say,'O Muhammad, lift up your head and speak, for you will be listened to, and intercede, for your intercession will be accepted, and ask for it will be granted:' Then I will raise my head and glorify my Lord with certain praises which He has taught me.

当我看见他，我会掉下来，在虚脱在他之前，他将离开我，只要他会，然后他会说，澳穆罕默德，就把你的脑袋和发言时，你将聆听，并交涉，对你的干涉，将不获接纳，并要求，将给予：'，然后我会提出我的头和颂扬我的主与某些称赞他教我。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。