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The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

I want to shoulder responsibility to the oneself, do not discommode the my body hair skin, the thought affection, living well, wanting to cry and then cry, wanting to smile and then smile, wanting to love for the love, want to hate and then hate, send the oneself a good heart feeling every moment and as far as possible.

我要对自己负起责任，别委屈我的身体发肤、思想感情，好好地活，想哭就哭，想笑就笑，想爱就爱，想恨就恨，时时刻刻尽量送自己一份好心情。

Let be a homeomorphism, the following statements were obtained: if is distal and has POTP ,then does not have the average shadowing property; if is equicontinuous and has the average shadowing property, then f is minimal; let :X→X be distal and chaintransitive, then does not have POTP; is distal if and only if is distal.

设是个同胚映射，得到了如下结果：若有POTP且是distal的，则不具有平均跟踪性；若有平均跟踪性且是等度连续的，则是极小的；若是distal的且是链可迁的，则不具有POTP；是distal的当且仅当是distal的。

A husbandman who had a quarrelsome family ,after having tried in vain to reconcile them by words ,thought he might more readily prevail by an example .so he called his sons and bid them lay a bundle of sticks before him .then ,having tied them into a fagot, he told the lads ,one after the other ,to take it up and break it .they all tied ,but tried in vain .then untying the fagot ,he gave them the sticks to break one by one .this they did with the greatest ease .then said the father:"thus you ,my sons ,as long as you remain united ,are a match for all you enemies ;but differ and separate ,and you are undone."

一个农夫，家庭不和睦，用言语劝告大家和好，总是无效，最后他想，用实际例子可能容易说服他们。于是他把儿子都叫来，吩咐他们抱一捆火柴放在他的面前。他把火柴捆成一捆，接着叫这些孩子挨次拿起来折断它，一个个都试过了，但都没有折断，接着他解了捆，一根一根地给他们折，他们都轻而易举地折断了。这时父亲说："孩子们，只要我们团结一致，你们就能对付一切敌人；但是如果意见不合，各自东西，那么你们一定要失败。"

So on to talk about the fire is now the 3rdgeneration ghost dance bar: According to squat key Do not let go and then press the button after the and then aspace Remember After jumping more than most bond by 0.1 seconds, and then at the same time go by this, of course, donot take, such as squatting ground so fast at the same time opening up again after the key and space squat orabove to continue in accordance with this kind of Methods jump This is the 3rd generation ghost jumped.

所以就说说现在最火的3代鬼跳吧：按蹲键不要松开然后先按后键再按空格记住后键最多比跳多按0.1秒，然后同时按这不放，当然蹲也别放等快落地使再同时放开后键和空格蹲还是按这再继续上面那种方法跳这样就是3代鬼跳了。

Explicitly, they are consist of three classes: If H is semisimple, then H k* for some finite group; If H is not semisimple and the characteristic of k is zero, then H is isomorphic the dual of the cross product between one so called Andruskiewitsch-Schneider algebra and a group algebra; If H is not semisimple and the characteristic of k is not zero, then H is isomorphic the dual of the cross product between one special algebra and a group algebra.

具体地讲，它们共分三类：①如果H是半单的，则H同构与一个群代数的对偶；②如果H是非半单的并且基础域的特征是0的话，则H同构一个所谓Andruskiewitsch-Schneider代数与一个群代数交差积的对偶；③如果H是非半单的并且基础域的特征不是0的话，则H同构于某个特定代数与一个群代数交差积的对偶。

In Russia, the range margin starts from the Chinese border near the confluence of the Zeya River into the Amur River (Blagoveshchensk City: 5015'N, 12734'E) and a little westwards, then runs northwestwards and northwards by the valley of Zeya River and its tributaries to the system of the Aldan River in Yakutia, then northwards to Ust-Aldan District and then turns eastwards to Tompon District Khandyga Settlement: ca.

在俄罗斯，范围利润率从中俄边境附近的合流的泽亚河进入阿穆尔河（布拉戈维申斯克市： 50 ° 15'N ， 127 34'E ）和一个小向西，然后运行西北和北方的河河谷泽亚及其支流的系统阿尔丹河雅库特，然后向北到乌斯特卡阿尔丹区，然后轮流向东至Tompon区（ Khandyga解决：晚上。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The user insets the money into the slit, and then the coin recognizer identify the inserted money and add it up; then the MCU sends out the signals about sellable goods by lighting the pilot lamp, so that the user can choose what he or she wants to buy; following the user presses the key related the goods which he or she wants to buy, and then the key makes single chip to start relevant parts to push out the goods, and now the buyer can get the goods. If there still left enough money, the user can buy another goods.

用户将货币投入投币口，货币识别器对所投货币进行识别；控制器根据金额将商品可售卖信息通过选货按键指示灯提供给用户，由用户自主选择欲购买的商品；按下用户选择商品所对应的按键，控制器接收到按键所传递过来的信息，驱动相应部件，售出用户选择的商品到达取物口；如果还有足够的余额，则可继续购买。

This invention discloses a user data process method, which contains 1, judging each data package in IP data steam sent form high layer of empty command protocol stack, if the received IP data package is TCP ACK data package, then executing step 2, if the received data package is not TCP ACK, then executing step 3, 2, making PDCP layer function process to IP data package and ending current process, 3, making flow control to IP data package and then executing 2,, said invention can precedential send TCP ACK data package to opposite part in preserving original TCP/IP and universal mobile telecommunication system data transmission system base, to reduce data package reversion time delay and keep stable data transmission rate.

本发明公开了一种用户面数据处理方法，其主要处理步骤如下：a。对从空口协议栈中高层下发的IP数据流中的每一个数据包进行判断：当前接收到的IP数据包是否为TCP的ACK数据包，对所有是TCP的ACK数据包的IP数据包执行步骤b；对所有不是TCP的ACK数据包的IP数据包执行步骤c；b。对IP数据包进行PDCP层的功能处理，结束当前处理流程；c。对IP数据包进行流控，再转入步骤b。采用本发明方法能够在保持原有TCP/IP和全球移动通信系统数据传输机制的基础上，优先将TCP的ACK数据包发送到对方，从而减少TCP的ACK数据包的回复时延，保证稳定的用户面数据传输速率。

Article 144 The Government of the Hong Kong Special Administrative Region shall maintain the policy previously practised in Hong Kong in respect of subventions for non-governmental organizations in fields such as education, medicine and health, culture, art, recreation, sports, social welfare and social

第一百四十四条香港特别行政区政府保持原在香港实行的对教育、医疗卫生、文化、艺术、康乐、体育、社会福利、社会工作等方面的民间团体机构的资助政策。原在香港各资助机构任职的人员均可根据原有制度继续受聘。

Small wonder, then, that the Chinese spend more in the shop than any other group of foreign visitors do .

这样的小惊喜，使中国顾客比任何国家的人消费得更多。