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The purified N protein was used to coat 96-well plate, each step was optimized, such as coating concentration of recombinant nucleocapsid protein, scample dilutim, chromogen and stop solution as well as concentration.

在此基础上，以纯化的重组N蛋白作为包被抗原，对各种条件进行优化（如抗原的包被，作用时间及底物的选择），确定了判定标准，建立了检测PRRS抗体的间接ELISA方法。

There are mainly three reasons for doing so: first, the readers of financial periodicals are much easier to be distinguished from other bourgeois readers; next, the operational ways of financial periodicals portray vividly in which field medium are investing in recently, this phenomena could be counterevidence to prove the powerful influence of bourgeois periodical on the society as a whole; finally, common problems in all the bourgeois periodicals are easier to be discerned in financial ones. The choke point that might well happened in the process of orientating a certain periodical is the object of being orientated, that is, the recipients, who are human beings equipped with infinite-possible mind and infinite-potential body, as well as self-initiative consciousness and self-determining behaviour.

论文选取目标人群为中产阶层的财经期刊作为研究对象的原因有三：一是财经期刊的消费主体较其他类型的期刊来说更集中于本论文所界定的中产阶层；二是财经期刊的运营很能说明近期的媒体投资热现象，而这一现象可以证明中产期刊的总体社会影响力不可小觑；三是财经期刊品牌建设是期刊产业化链条思路的有力代表。

A new analysis system for enrichment of glycoproteins/glycopeptides as well as the identification of glycoproteins/glycosylation sites was also established by coupling enrichment of glycopeptides by hydrazide- functionized magnetic microspheres with release of polysaccharide chains by PNGase F and mass spectrometry as well.

本实验还将肼功能化磁粒富集糖肽方法与糖肽酶酶解、生物质谱等方法相结合，建立起了一套可用于糖蛋白/糖肽分离富集并进行糖蛋白/糖基化位点鉴定的新体系。

Lianqiu entertainment products co.,ltd, lying in YongKang City ,which is well-known as the Capital of Hardware in China, is specialized in designing,producing and marketing Fishing tackles that cover from landing net, keeping net to gaff as well as sunshade and beach chair.

猎鱼者钓具是浙江连球工贸公司旗下从事渔具与休闲品研发、生产、销售于一体的企业。产品主要有：钓台、钓箱、鱼钩、钓线、抄网、支架、鱼护、钓鱼伞、沙滩椅、鱼竿包、渔具盒等钓具产品。

Products such as Nike Jordan and Puma sell well in America , Canada , as well as Europe and other countries.

产品，如耐克约旦和美洲豹出售，以及在美国，加拿大，以及欧洲及其他国家。

Well-known enemies like the Titanium Man and the Crimson Dynamo were considered, but finally the lesser-known Iron Monger, Obadiah Stane, was chosen as Iron Man's adversary (Stane, as well as possessing his own armor, is also a business contemporary of Stark).

著名的敌人，如钛甲人和绯红机甲，都在考虑范围内，但是最终名气较小的铁霸王，欧巴迪亚·斯坦恩，被选为了铁人的对手（斯坦恩不仅拥有他自己的盔甲，他也是斯塔克的经济合伙人）。

In other species it has a protective as well as a sexual function, and in some species, it is important in thermoregulation as well.

在别的物种，它有保护和性方面的作用。在某些物种，它在体温调节中有重要作用。

In a setting like Korea it will definitely improve your tourney ability and confidence as well as there are more chances for a next shot easing the pressure. Though they have many other, more substantial pressures to deal with as well.

在像韩国联赛那种大环境中你当然能提高你的比赛能力和自信心，这里还有更多的机会来缓解压力尽管他们还有其他的，潜在的压力要处理。。

Today, Spanish architects have growing influence throughout the world, as well as on Iberian peninsula, as a recent exhibition at New York's Museum of Modern Art amply demonstrated. The roster of twentieth-century and contemporary Spanish singers is impressive and well-known, among them Victoria de los Angeles, Montserrat Caballe, Alfredo Kraus, Teresa Berganza and, of course, Placido Domingo, who is also claimed by Mexico.

无论是在利比亚半岛，还是纽约现代艺术博物馆都充分证明了西班牙的建筑家们在全世界拥有越来越大的影响力。20世纪和当代西班牙歌唱家的名单也可谓是群星璀璨，有维多利亚&德&洛斯&安赫莱斯、蒙茨克拉特&卡巴耶、阿尔弗雷多&克劳斯、泰瑞莎&贝尔甘札，当然，也少不了多明戈。

In order to obtain a correct conception of the Mishnah, as well as of its value and importance, it is necessary to consider its relation to preceding collections of similar content as well as the general development of the oral doctrine from the earliest midrash of the Soferim down to the time when the Halakah received its final form.

为了获得正确的概念米示拿，以及其价值和重要性，有必要考虑其有关前收藏的类似内容以及一般发展理论的口头从最早的米特拉斯的Soferim到时候， Halakah收到它的最后形式。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。