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All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

This paper discussed a series of pre-processing theory and an algorithm for analyzing document image of information card based on optical mark recognition.

阐述了信息填涂卡文档图像标记识别系统中的预处理算法。

This paper discussed recognition algorithms for analyzing document image of information card based on optical mark recognition, and proposed a new information card fill-in method.

阐述了基于图像识别的信息填涂卡文档图像标记阅读机中的识别算法，并提出一种新的信息卡填写方式。

This paper discussed a series of pre-processing theory and an algorithm for analyzing document image of information card based on optical mark recognition.

阐述了信息填涂卡文档图像標记识别系统中的预处理算法。

Discussed a series of pre-processing theory and algorithms for analyzing document image of information card based on optical mark recognition,and proposed a new information card fill-in method.

阐述了信息填涂卡文档图像标记识别系统中的预处理算法，并提出一种新的信息卡填写方式。

The spectrum features on wheat canopy were studied by measuring and analyzing the reflectance and differential spectrums at 5 infested levels by wheat aphid.

通过对田间不同程度麦蚜危害的小麦冠层反射光谱测量，研究了各波段反射光谱的变异特征，并对光谱反射曲线进行微分分析。

By analyzing the images and the work function change, we propose a new scheme for this well-studied system.

对STM图像及功函数变化的分析表明，当Na原子覆盖度小于临界覆盖度(0 。0 8ML)时，Na原子具有类气态的性质并可以在一个吸附能阱中快速移动。

The results show that amplitude of defect signal is adversely affected by lift-off variations,but phase difference between defect signal and lift-off loci is almost unchangeable.After analyzing the characteristics of the phase difference with the chang of frequency,a conclusion that defect signal is approximately vertical to lift-off noise at a well-selected frequency is obtained.

结果表明提离增加会显著降低缺陷信号的幅值，但并不影响缺陷信号和提离轨迹之间的相位差，通过分析相位差随检测频率的变化规律发现：存在一个合适的频率可使缺陷信号和提离干扰之间出现90°的相位差。

Secondly,this paper establishs the detailed program of test and tracks the operation of this network system. By analyzing the practical running data,this paper concludes that the system is well-operated.

论文接着对该网络系统的验收测试制定了详细的计划并且对网络实际运行进行跟踪，通过对实际运行数据的统计分析，得出运行效果优良的结论。

OOA(object-oriented analysis) aims to build a analysis model based on class diagram..but among most OO methods,OOA lacks formal,unified and well-operated measures, only directly finding class diagram by means of analyzing problem domain through domain experience.

OOA的目的是建立以类图为核心的分析模型，但这些OO方法的OOA阶段对于建立类图大都缺乏规范、统一、可操作性强的手段，只是凭借领域经验通过分析问题域直接得到类图。

In other research, chondroitin is being investigated for use in several other conditions.

在其它研究，软骨素被调查用於几个其它情况。

They would make fun of him for having to work.

他们拿他的工作来开玩笑。