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Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

The common method, that all strong-correlation terms of the model are eliminated, can bring the loss in the engineering application, so the new method is proposed that the identified model reserves some correlation. The augmented matrix A is constructed by the outputΔW and the matrix S. The"determinating order based on ratio of determinant"is brought out to screen the strong-correlation terms in the structure identification. The latent root estimation is improved in screening the eigenvalues and eigenvectors. Thus the estimation precision is improved greatly.The consistence check of guidance instrument error coefficients of flight test and ground test is the purpose of flight experiment. The causes of inconsistency of the two models are analyzed. The hypothesis test of linear regression model based on F statistics is proposed to check the consistence.Finally, the instability of error coefficients is probably caused by the change of the flight environments, therefore, the relation between the error coefficients and flight environment is analyzed. The approach is presented to identify SINS guidance instrument error models and compensate the error in the segmented sections corresponding to the change of vertical acceleration of aircraft.

在结构辨识中，常用的方法由于将模型中的强相关项全部剔除而给工程应用带来损失，因此，本文提出了新的有益思想，即在保留一定相关性的基础上进行辨识：将输出向量ΔW与环境函数矩阵S构成增广矩阵A，然后采用"比定阶行列式"来剔除相关向量的方法，这样既可以尽可能多地保留了对落点影响大的强相关参数，又可以对落点影响小的强相关参数给予剔除；在参数估计中，改进了特征根估计中特征根和特征向量的筛选方法，提出"近零"准则，从而大大提高了参数估计的精度；再者，鉴于天地模型"一致性"检验是飞行试验和SINS制导工具误差系数分离的主要目的，因此，本文又深入分析了造成天地模型不一致的原因，提出了采用基于F统计的线性回归模型假设检验方法来进行捷联制导工具误差模型的天地"一致性"检验；最后，鉴于飞行环境剧烈变化可能会对惯性仪表误差系数稳定性带来一定的影响，因此本文深入地分析了SINS制导工具误差系数与外界环境的关系，提出了基于过载变化大小的分段辨识和分段实时补偿的算法。

Sampled by Tang Paradise, the basic characters of Tang Garden are analyzed and four characters are generalized , they are magnificence, splendor, austereness and with strong human atmosphere;the aesthetic and innovational characters of culture design ideas, the landscape ecology and innovational characters of ecology design ideas, the geographic landscape and innovational characters of planning design ideas are all analyzed in the paper,.

本文以唐芙蓉园为例，尝试分析唐代园林的基本特征，归纳为八个字：大气、壮丽、质朴、赋文四大特征；以大唐芙蓉园为例，分析其文化设计理念的美学特征及其创新特色；分析其生态设计理念的景观生态学特征及其创新特色；分析其规划设计理念的地景学特征及其创新特色。

On this basis,back electromotive force without load and with load is researched,simulated and analyzed,and cogging torque is analyzed and calculated in different dimensions of magnetic steel.

在此基础上，仿真了空载和负载下的电机反电动势，并且对不同磁钢尺寸下的定位力矩进行了分析计算。

The problems of teaching administration are investigated and analyzed, it is reachered and analyzed that domestic and abroad course arranging arithmetic such as heredity arithmetic, simulation-back arithmetic, expert system arithmetic, backdate arithmetic and first class arithmetic. A point of view that the target result is only subordinate result is clanitied.

通过对中职学校的教务管理排课问题的调查分析和国内外排课算法(遗传算法、模拟退火算法、专家系统方法、回溯算法、优先级算法等)的对比研究分析，阐明了由于排课问题算法的时间复杂度极高，实现有一定的难度，求最优解是理论上的，目标解只能是次优解的观点。

In this paper, the dynamic compressing experiments for the matrix material of the tungsten alloys which have been three different deforming(0%、 32.6%、 44.6%) processed have been performed by the experimental technology of separated Hopkinson pressure bar and many accurate datum have been obtained. Also the three materials have been compared with each other and analyzed. The results show that the optimized values are in a certain range to optimize the materials by changing their deforming technology. The parameters of constitutive model of Johnson-Cook have been fitted based on the datum. Then proceed to the next step, adiabatic shear band of these three matrix materials has been analyzed with finite element method. The result shows that the relations between stress and strain are different because of the differences of technical process of the three materials, but they are in great commonness in the material experiment and in great coherence in the distribution of time and space, which can been seen from the space distributing figures of plastic strain.

利用分离式Hopkinson压杆实验技术对三种不同变形量(0%、32.6%、44.6%)钨合金基体材料进行动态压缩实验，取得大量精确的数据，对比分析三种材料，结果表明通过改变变形量对材料进行优化，其变形量的优化值是有一定范围的；本论文拟合、确定了钨合金基体材料Johnson-Cook本构模型参数并对三种基体材料绝热剪切带进行有限元分析，结果表明三种材料由于工艺流程的不同，应力应变关系也不尽相同，但从塑性应变的空间分布图上体现了材料很大的共性和时间以及空间分布的一致性。

To give the definition of the mass concrete and to concrete\'s cracks together with practical meanings of control ,and give a brief review on the research in the field at home and abroad, at last, methods to control temperature crack of the mass concrete from several aspects such as design measures,concrets material, construction schedules.etc are summed up; To explain the physical mechanics of the concrete and computing methods-Difference Method and Finite Element Method, and illustrate the application of their specific methods repectively;Several temperature factors that can influence the temperature stress of mass concrete baseplate are analyzed. The influence degree of temperature factor can be got through the ANSYS simulation computation the control of temperature can control the temperature stress, consequently control the temperature crack of mass concert baseplate; through the influence of the crack resistance ability of mass concrete baseplate which takes the reinforcement as a part is analyzed. By ANSYS simulation, the best suited volume ratio of reinforcement is found out, at same time the layout of reinforcement with the same volume ratio can also affect the resistance ability of mass concrete baseplate. Sum up of above analysis, anti-crack function of steel bar in early mass concrete baseplate is obvious, so it\'s necessary to consider beneficial effect of construction steel bar in research of temperature control on mass concrete baseplate.

本文先容了大体积混凝土及混凝土裂缝的基本概念，对国内外在这个领域里的研究现状作了扼要叙述，并从结构设计、混凝土材质、施工措施等方面总结了大体积混凝土温度裂缝控制的方法及防止措施；阐述了混凝土的基本物理力学性能，总结了大体积混凝土温度计算的方法——差分法和有限元法，并分别举例说明其具体应用的方法；分析了影响大体积混凝土底板温度应力的几种温度因素，利用ANSYS模拟计算，得出了每种因素的影响程度，通过分析提出了在这几种温度影响因素下如何通过控制温度以达到控制温度应力从而来控制裂缝的具体建议；针对大体积混凝土基础底板中配筋的不同对混凝土底板抗裂能力的影响不同，利用ANSYS进行模拟分析，得出底板中最合适的体积配筋率，同时分析了在相同体积配筋率下钢筋的具体布置对大体积混凝土基础底板抗裂能力的影响，分析表明，配置钢筋对混凝土结构的开裂阻止作用明显，在研究大体积混凝土温度裂缝题目时，要考虑钢筋在其中的有利作用。

The main research results in this dissertation can be given as following:Firstly, the bidding strategies and affections of generation companies on the TOU power price are analyzed; Supply function model is employed to simulate the bidding strategies of generation companies in power pool. Some meaningful results are obtained through the proposed equilibrium equations model, when different bidding parameters are selected to maximize profit of suppliers, such as the the numeral of generation company, the block bidding, and power demand elasticity. Based on these results, the affections between the bidding strategies and the TOU power price are discussed.Secondly, the important principles consider the factor of bidding strategies of generation companies and consumers gaming strategies are proposed to constitute the new TOU power price model under present electricity market. Based on these pricinples a new mathematical model of TOU power price is constructed, to evade electricity market risk, partition the peak-valley, ascertain the consumers' response curve, and protect the ambilateral profits.Thirdly, the affections of the TOU power price strategies for reducing the network loss, adjusting node voltage, improving load curve of power system, and protecting the consumers' benefits in electricity market are analyzed with applications of a city real time load data of Jiangsu province.

针对"厂网分开，竞价上网"的电力市场运营模式，本文主要完成了以下研究工作：1研究了发电商不同的竞价上网策略，利用供给函数均衡方法，建立了发电商的竞价上网策略模型，给出了市场均衡解的具体解法；讨论了不同条件下发电商的竞价策略对市场的影响，并获得了发电商的最优上网竞价策略，明确了竞价上网与峰谷分时电价之间的影响因素；利用电力系统负荷曲线，建立了发电商最优竞价策略与峰谷分时电价之间的相互联系，通过仿真算例分析了峰谷分时电价与发电商最优报价之间的相互影响。2提出了"厂网分开，竞价上网"电力市场模式下，考虑发电侧竞价和用户侧博弈等风险因素影响，峰谷分时电价理论建模在规避电网企业运营风险，保护供电方与用户双方的利益、确定用户响应曲线、划分峰谷时段、设置合理的电价拉开比等方面所应遵循的基本原则，在此基础上建立了适合电力市场模式的峰谷分时电价模型。3从原理上分析了需求侧实行峰谷分时电价策略，对削峰填谷，提高负荷率，改善负荷曲线形状，降低电力系统的电能损耗和电压损耗等方面的影响，并进行了仿真验证。

The influence of those main design parameterssuch as evaporation saturation temperature t_s、heat dissipated power Q and charge ofworking fluid L on the temperature distribution of evaporation"s bottom and boiling heat transfer inside the evaporation has been calculated and analyzed concretely. The method that uses channels to enhance boiling heat transfer of evaporation"s bottom has also been analyzed and calculated preliminarily.

具体计算和分析了蒸发饱和温度t_s、散热功率Q和充液量L等主要设计参数对蒸发段底面温度分布和蒸发段内部沸腾传热特性的影响，对用槽道强化蒸发段底面沸腾换热也做了初步的数值计算和分析。

This paper describes the history of urban land price reform and the evolution of land market system. The characteristics and mechanism of land price are analyzed in line and branch. By taking Nanjing City as an example, the status quo and existing problems of Chinas urban land price system are revealed and analyzed.

本研究描述了我国城市土地价格改革的历程和土地市场形成过程，分析了土地价格的形成特点和形成机制，并结合南京市的实际阐述了目前南京市城市土地价格体系的现状和存在的问题。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。