查询词典 amylase

By comparing the activity of theα-amylase and the soluble acid invertase in the kernel and the pedicel, the trend of theα-amylase activity in the kernel and the pedicel showed a peak, theα-amylase activity was high in the early stage, it could promoted amylolysis, supply the nutrition for the kernel development; theα-amylase activity was low in the rapid filling stage, it was in favorable to the starch accumulation.

通过比较籽粒和小穗柄中α-淀粉酶和可溶性酸性蔗糖转化酶的活性表明，籽粒和小穗柄中α-淀粉酶活性均呈单峰变化趋势，籽粒发育早期α-淀粉酶活性较高，可以促使淀粉转化，为籽粒的早期的发育提供营养物质；籽粒发育中期为快速灌浆期，淀粉酶活性较低，利于淀粉的积累。

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

Though monitoring of the bacteriolysis of lysozyme to M. Lysodeikeicus and the hydrolysis of soluble starch catalyzed by α-amylase, lysozyme and α-amylase in human saliva were determined.

通过溶菌酶对溶壁微球菌的溶菌作用及α-淀粉酶对淀粉的水解作用，成功地测定了人体唾液中的溶菌酶和α-淀粉酶。

The principle component analysis showed that head rice ratio, amylose content, chalkiness ratio, gelatination consistency and protein content were most quality traits in low amylase content (5-15%), and head rice ratio, amylose content and chalkiness ratio were most quality traits in low amylase content (5-15%). Correlation of amylose content and 8 agronomic traits showed that: if single plant of low amylase was selected, its'agronomic traits would be affected in negative. Tassel time of low amylose content in 3 combinations was early in their tassel time, and it was important time to select it.

通过主成分分析，可以把整精米率、直链淀淀粉含量、垩白米率、胶稠度和蛋白质作为F2代选择低直链淀粉含量(5-15%)单株的主要品质性状，把整精米率、直链淀淀粉含量和垩白米率作为F3代选择低直链淀粉含量(5-15%)单株的主要品质性状，从而提高选择效率。3个组合类型中，选择低直链淀粉含量较低的单株时，其农艺性状会受到负面影响。3个组合类型低直链淀粉含量单株的抽穗日期都是在每个组合的抽穗早期，因此这是选择低直链淀粉含量单株的重点时期。

Significantly genotypic differences and great variations were detected in grain β-amylase activity,β-glucan and protein fraction contents. The largest variation and highest average value of β-amylase activity were found in Hangzhou 2006-2007, while the trend of coefficient of variation was opposite to the mean content of β-glucan over different environments. Hordein content had the highest correlation coefficient with β-amylase activity among four protein fractions.

研究结果表明：籽粒β-淀粉酶活性、β-葡聚糖和蛋白组分含量均存在显著的基因型差异；杭州2006-2007年度种植条件下的β-淀粉酶活性均值和变异幅度最高，且β-淀粉酶活性和β-葡聚糖含量的变异系数和变化趋势恰好相反；4种蛋白质组分中，醇溶蛋白含量与β-淀粉酶活性呈显著正相关。

The study suggested that the optimal method not only enhanced the affinity and the maximum reaction velocity of α-amylase with its substrate, but also improved the accuracy and the sensitivity on the activity of α-amylase from M. separata larvae in assays, whereas it was not the optimal method for determining the activity of α-amylase from T. molitor, C. pipiens pollens and M. domestica larvae.

结果说明，在该优化体系下，粘虫α-淀粉酶与底物的亲和力增强，最大反应速度增大，测定酶活性的准确性和灵敏度显著提高；同时该优化体系也可作为测定德国小蠊α-淀粉酶活性的优化方法，但不适合作为黄粉虫、淡色库蚊和家蝇α-淀粉酶的最优化测定方法。

Methods The acute necrotizing pancreatitis model was made by retrograde injection of3.5%Sodium Taurocholic Acid into the rat's pancreatic duct.Through mirabilit for external application and qing yi decoction,the changes of serum endothelin,nitric oxide,amylase and the weight changes ofmirabilit for external application and the pathological changes of pancreas,lungs were observed.

应用牛磺胆酸钠逆行胰胆管穿刺注射造成大鼠急性坏死性胰腺炎（acute necrotizing pancreatitis，ANP）模型，通过芒硝外敷与清胰汤内服联合应用，观察大鼠血清内皮素（endothelin，ET）、一氧化氮（nitric oxide，NO）、血清淀粉酶（amylase，AMY）的变化及芒硝外敷前后自身重量的变化和胰腺、肺脏的病理变化。

To examine the hypothesis, we test whether maize beta-amylase can serve as a lipase inhibitor.In order to conduct the interaction assay of beta-amylase and lipase, we adopted a preparative electrophoresis method with a Prep Cell to purify beta-amylase proteins from germinating maize endosperms and soybean cotyledons. We pre-mixed tested proteins with lipid substrate prior to the addition of lipase preparation and assayed the lipid hydrolytic activity.

本实验利用Prep Cell纯化玉米萌芽谷粒和大豆子叶中的beta-淀粉酶，分别将两种不同物种的beta-淀粉酶与胰脂解酶预先反应后，再加入脂解酶反应液中观察活性的变化，结果发现脂解酶的活性未如预期，没有抑制的效果产生，将甘薯beta-淀粉酶以同样方法与胰脂解酶作用，结果同上述两种beta淀粉酶依旧没有抑制的现象。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。