查询词典 ampullate

Objective To further understand the intracellular calcium mobilization features of the isolated ampullate crista vestibular hair cells and ATP effects on intracellular free calcium concentration(［Ca2+］i) in VHCs of the guinea pig.

目的 探讨离体前庭毛细胞（vestibular hair cells，VHC）去极化时细胞内钙离子活动特征及内耳活性物质ATP对离体VHC内钙离子浓度的影响及其机制。

The major ampullate dragline silk of spiders such as Araneus diadematus and Nephila clav

蜘蛛丝的这些性能和特点与蜘蛛的生态策略、生活史及蛛丝蛛网的结构和功能密切相关。

Dragline spider silk produced from Nephilia clavipes major ampullate is a natural fibrous protein with specific me- chanical properties such as high tensile strength and elasticity.

蜘蛛大壶腹线产生的拖丝是非常优良的纤维蛋白，具有独特的强度和弹性。

To realize the molecular compositions and bio-spinning mechanisms of spidroin in spider major ampullate, the molecular weight and amino acid compositions of spidrion solution were measured and compared with that of dragline silk. The molecular conformations and morphologies of spidroin and dragline silk in different spinning phases were determined by circular dichroism spectroscopy and laser Raman spectroscopy.

本文以广泛分布于我国各地的大腹园蛛为对象，研究了大腹园蛛主腺体内丝蛋白溶液的分子量和氨基酸组成，并利用圆二色光谱和拉曼光谱分析了蜘蛛在不同成丝阶段丝蛋白的分子构象和聚集态结构的变化，以期了解蜘蛛主腺体内丝蛋白的分子组成及其生物纺丝机制。

In addition, the reconstructed phylogenetic tree based on cDNA and DNA sequences encoding C-terminal non-repetitive region of MaSp1 (Major ampullate spidroin-1) also showed that Deinopoidea and Araneoidea are not sister groups.

另外，依据编码大壶状腺丝蛋白-1(MaSp1)C末端非重复氨基酸序列区段的核酸序列重建的系统发生树也证实圆网蛛类并非单系发生。

Keywords: Key wodrs:Araneus ventricosus;Major ampullate gland;Flagelliform gland;cDNA library;Spider silk protein mRNA

中文关键词：大腹园蛛；主壶腹腺；鞭毛腺；cDNA文库；蛛丝蛋白mRNA

This study separated high quality of steel and high elastic silk gene of major ampullate gland and flagelliform gland of Aranbeus ventricosus,and saparated mRNA ,and published several new gene of silk protein for AvF1(AY174110),AvF2(AY177203),AvFf1(AY587193) in GeneBank.

通过鸟枪法、Southern杂交等方法筛库，在没有借鉴国外丝蛋白基因的基础上，获得具有自主知识产权的蛛丝蛋白mRNA，并将蛛丝蛋白AvF1(AY174110)、AvF2(AY177203)、AvFf1(AY587193)等多个新基因登录GenBank。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。