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The data byχ~2 were consistent with the separation ratio as 1: 2: 1 χ~2=4.028＜χ_(0.05,2~2=5.99, which further conformed that glandless character was controlled by a incomplete dominance gene, Gl_2~e. 2. SSR markers every about 5cM were selected in the chromosome 12 of our lab genetic linkage map to screen the parent plants. The markers with the polymorphism were used to amplify the DNA of 210 F_2 segregating plants selected randomly and recorded the marker genotypes. After eliminating the segregation distortion markers byχ~2 test, other SSR markers were detected by linkage analysis.

二、参照本实验室海陆种间遗传连锁图谱第12染色体上的SSR标记，平均每5cM左右选择一个标记进行亲本多态性的筛选，筛选出具有多态性的标记位点进一步扩增随机抽取的210个F_2群体单株的DNA，鉴定每个个体的标记基因型。X~2检测剔除偏分离标记后进行连锁分析，发现Gl_2~e基因位于CIR362与NAU5079之间，进一步参考图谱上位于CIR362与NAU5079之间的标记加密图谱，最终将其定位在CIR362和NAU2251b、NAU3860b、STV033之间。

The upstream regulatory region of the storage protein was separated from Glycine max variety "Ji Nong T7018" genomic DNA, according to published glycinin 11S G7 subunit (Gy7) gene(GenBank AF319776) sequence, a pair of primers was desgeined, in order to amplify the array before Gy7 gene initiation codon.

我们以大豆品种&吉农T7018&的基因组DNA为模板，根据已发表11S球蛋白G7亚基(Gy7)基因(GenBankAF319776)序列，设计一对引物，通过PCR扩增得到Gy7基因的启动子序列。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

据文献报道及GenBank中注册的基因序列，运用DNAStar软件分析同源率及Primer5.0软件对Tm值、G+C含量、引物二聚体等进行分析，根据Ⅰ类整合子的结构设计了3对引物，对Ⅰ类整合子的intI1、可变区、3'保守端进行扩增，检测沙门氏菌和大肠杆菌Ⅰ类整合子；扩增出了相应基因片段并测序；61株沙门氏菌和48株大肠杆菌中分别有50株和32株菌检测出Ⅰ类整合子，Ⅰ类整合子的检测率分别为：81.97％和66.67％。

ERIC, a pair of rep-PCR primers, can amplify NSa-specific band. Using the amplification band patterns of two pairs of primers, which are REP and ERIC, six types of male sterile cytoplasm can be distinguished. 2. A cDNA library for CMS-associated genes was constructed by suppression subtractive hybridzation with the sterile line and its isonuclear maintainer.

利用特异引物扩增的结果表明NSa不育胞质与Pol、Ogu不育胞质不同，一对rep-PCR引物ERIC能扩增到NSa的特异带，而且REP和ERIC两对引物相结合能将NSa不育胞质与油菜原有的不育细胞质区分开，从分子水平上证明了NSa不育胞质的新颖性。

To clone the calmodulin genes from Karelinia caspica, Kalidium foliadum and Betula halophila in Xinjiang, designed primers were used to amplify 450-bp cDNA fragments with RT-PCR.

提要 采用RT-PCR扩增的方法，从新疆盐生植物花花柴、盐爪爪和盐桦中分别克隆获得了450 bp的cDNA片段。

3When subjected to the seismic ground motion of the shock type, the shallow, thin and narrow liquefied lenticle may amplify the response of the building with short period.

计算结果表明：(1)所采用的方法与已有的模型实验结果有很好的对应关系，可用于分析建筑物存在下的场地砂土液化问题；(2)饱和砂土透镜体液化对短周期建筑物主要起到隔震作用，且随着透镜体厚度、埋深和宽度的增加以及场地输入地震动加速度的增大，减震效果有所增加；(3)在冲击型地震动作用下，埋深浅、宽度和厚度小的液化透镜体有时却对短周期结构加速度反应起到增大作用。

The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma , then the purified PCR productions were cloned into T-vector and sequenced by M13+/-primers.

以特异性引物扩增了金焰笛鲷的核糖体第一转录间隔区（ITS-1），扩增产物经克隆后测序，测得ITS1长度为566 bp。

Forty microsatellite loci were selected to amplify the genomic DNA of cultured stock of largemouth bass Micropterus salmoides L.

本研究在人工养殖的大口黑鲈（Micropterus salmoides L。

Base on them, credit riskdegree is brought forward and treated as a new criterion (model's output).Comparing with other typical criteria, traditional ones all fall short of the fouressential attributes in some degree. Especially, when the continuity could not bemet, criteria will fluctuate seriously, which amplify actual credit risk for a whileand minify it for a moment. So the evaluating results are distorted severely.

以此为基础，提出以信用风险度作为新的衡量标准，并分别与典型衡量标准进行了比较分析，发现传统信用风险衡量标准，在这四个基本属性方面都存在不同程度的弊端与不足，尤其在不满足连续性时，衡量标准具有严重的&波动性&，体现为对实际信用风险忽而不同程度地放大，忽而不同程度地缩小，严重扭曲了风险评估结果。

Methods Eight STR primers were used to amplify DNA in the three outbred stock zebrafish.

方法利用5对斑马鱼STR引物和3对剑尾鱼STR引物，优化PCR条件，对不同种群斑马鱼DNA进行扩增。

Diabetes is a social disease that affects several million people worldwide.

糖尿病是一个社会性疾病，全世界有数百万人罹患此病。

I'll call you on Friday to see if we can reschedule our luncheon meeting at your convenience.

我星期五会给您打电话在您方便的时候我们重新安排我们的午餐约会。