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Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

If you only have an old turntable and no amplifier, you will need to amplify the signal using a pre-amplifier before plugging it into the PC sound card.

如果你只有一个老唱盘，没有放大器，你需要使用前置放大器将信号放大，然后再将其与电脑声卡连接。

Depended on the sequence of the band of 489bp, a pair of prime was designed to amplify the specific band of 355bp for all the seven strains.

根据489bp条带序列设计引物，能特异性在所有7个白叶枯菌株中扩增出355bp的条带。

The analysis showed: socialized service demand of forestry or potential demand is vigorous overall after the reform of forestry propery right system, socialized service system of forestry was need to set up and amplify objectively. Socialized service of forestry ; Service system ; Social demand

为此，笔者与课题组成员于2006年8月至2007年9月先后深入江西省林业基层单位、乡村和农户中开展林业社会化服务需求调研，以期通过实地调研为林业社会化服务体系建设提供依据。1林业社会化服务体系的概念和内容林业社会化

The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.

检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性，最低可以检测到10个TCID50/100μl的病毒，说明该方法的有较好的特异性和敏感性。

Traditional media audiences are also more passive - online audiences can engage with, rebroadcast and otherwise amplify your communication efforts.

说的真实和公平的，如果您认为您的业务可以蓬勃发展的同时，采取这种态度的时期激烈的社会和经济变化，然后你刚才的岩石上，与您的坏的自我。

A pair of primers based on conservative sequences of Drosophila melanogaster was designed to amplify the p53 gene. The fragment with 1156 base pair was obtained and sequenced. The percent identity between others' and ours' was 99.4%, with the other biological sequence of p53 existing some differences.

从紫外刺激的果绳抽提总RNA，通过RT-PCR方法扩增得到有完整读码框的p53基因，并且通过对p53基因的序列分析表明，所得到的序列与网上已经登陆的果蝇p53序列有99.4%的相似系数，而与其他生物的p53序列存在一定的分歧。

Recent research findings show that under the circumstance of traumatic hemorrhagenic shock, in such viscera as liver, lung and small intestine, large numbers of polymorphonuclear neutrophil will congregate, sequestrate and even infiltrate, which result in tissue edema and inflammation. At the same time, due to the fact that PMN delays its apoptosis and can not be removed in time, secondary necrosis will occur and release great amount of toxic substances, such as absorbent oxygen and free radicals, which will amplify inflammation. All the above constitute the major cause of SIRS, MODS and MOF.

近来研究发现在创伤失血性休克的情况下，机体内各脏器普遍可观察到大量中性粒细胞的聚集、扣押甚至浸润，造成组织水肿，出现炎症反应，同时由于PMN凋亡延迟，不能及时被清除而发生继发性坏死，释放大量的活性氧、自由基等毒性产物使炎症反应进一步扩大，是创伤引发全身炎症反应综合征、多脏器功能不全甚至衰竭的主要途径。

Serine proteases amplify signals in many immune-related pathways and thus play a crucial role in innate immune system.

丝氨酸蛋白酶是先天免疫中至关重要的酶类，在许多通路中起信号放大作用。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。