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In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification, iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry, 2D-LC-MS/MS)对雌二醇(17β-oestradiol, E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较,共鉴定到置信度在95%以上的蛋白质576种,其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质,其中10种上调,16种下调。

Among 14 cases of trigeminal tumor,12 cases were examined by CT scanning,2 cases by MRI,all of them were confirmed by pathological examination.The density appeared heterogeneous with 6 cases,homogeneous high density with 5 cases and isopyknic with 1 case.Dropped contrast medium,the 12 CT scanning pictures appeared heterogeneous potentialization,and there were no cerebral edema around the tumor.Among 2 cases examined by MRI,T1 weighted image appeared low signal,T2 weighted image appeared high signal.

内容提要三叉神经瘤14例,12例做CT检查,2例做MRI检查,14例术后均经病理检查证实。12例CT扫描呈混杂密度6例,均匀高密度5例,等密度1例,注射造影剂后多呈不均匀强化,肿瘤周围均无脑水肿。2例MRI图像T1加权像呈低信号,T2加权像呈高信号。X线平片示11例有岩骨、颅底骨吸收破坏。

If, upon such liquidation, dissolution or winding up of the affairs of the Company, the assets of the Company distributable among the Holders of Series A Convertible Preferred Stock and any other series of Preferred Stock ranking on a parity therewith in respect thereto or any class or series of capital stock of the Company ranking on a parity therewith in respect thereto shall be insufficient to permit the payment in full to all such Holders of shares of the preferential amounts payable to them, then the entire assets of the Company available for distribution to such Holders of shares shall be distributed ratably among such Holders in proportion to the respective amounts that would be payable per share if such assets were sufficient to permit payment in full.

在公司进行该等清算、解散或清盘事宜时,如果在拥有A系列可转换优先股的持有人和拥有任何其它系列优先股(其优先股等级与可转换的优先股等级相等)的持有人或拥有公司任何等级或系列股本(其股本等级与可转换的优先股等级相等)的持有人之间可分配的公司资产不足以对所有这些股票的持有人(对他们应优先进行款支付)进行全额支付,则公司可用来分配给这些股票持有人的全部资产应以每股假设在该资产能够进行全额支付时的情况下应分别支付的数额在这些持有人之间按比例进行合理地分配。

If, upon such liquidation, dissolution or winding up of the affairs of the Company, the assets of the Company distributable among the Holders of Series A Convertible Preferred Stock and any other series of Preferred Stock ranking on a parity therewith in respect thereto or any class or series of capital stock of the Company ranking on a parity therewith in respect thereto shall be insufficient to permit the payment in full to all such Holders of shares of the preferential amounts payable to them, then the entire assets of the Company available for distribution to such Holders of shares shall be distributed ratably among such Holders in proportion to the respective amounts that would be payable per share if such assets were sufficient to permit payment in full.

公司资产中有可用于在A系列可转换优先股,以及与A系列股等价的其他相关系列优先股或其他相关种类或系列的公司股本股东之间进行分配的部分,在公司事务清算、解散或停业时,如果这一部分资产不足以对上述持有优先可支付额的股东进行全额支付,那么公司应调用全部可用资产,如果这些资产足够进行全额支付,则应根据此类股东各自的每股派息额数量按比例分配。

If, upon such liquidation, dissolution or winding up of the affairs of the Company, the assets of the Company distributable among the Holders of Series A Convertible Preferred Stock and any other series of Preferred Stock ranking on a parity therewith in respect thereto or any class or series of capital stock of the Company ranking on a parity therewith in respect thereto shall be insufficient to permit the payment in full to all such Holders of shares of the preferential amounts payable to them, then the entire assets of the Company available for distribution to such Holders of shares shall be distributed ratably among such Holders in proportion to the respective amounts that would be payable per share if such assets were sufficient to permit payment in full.

如果公司发生清算,解散或清盘,用于A系可转换优先股东,和任何其他等值优先股系,或任何种类,或任何等值相关股本系股东分配的公司资产应不足以全额支付该优先股份所有股东,则公司全部用于分配该股东的资产应按照每股应付的相应数额比例分配,如果该资产足以全额支付。

Among the 45 neurons generating phasic firing, 8 (17.78﹪) neurons could still be induced phasic firing after treatment with 1×10-2 μg/mL SVHRP and 37 (82.22﹪) neurons had no responses to the stimulation. The AP firing of neurons was dramatically different after treatment with SVHRP (P<0.01, n=45). Among the 7 repetitive firing neurons, all of them could only generate 1 or 0 AP instead of repetitive firing when SVHRP was applied. The number of APs was 14.57±1.00 and 0.57±0.20 before and after SVHRP treatment (P<0.01, n=7). The AP rheobase was (75.10±8.99) pA and (119.85± 12.73) pA before and after 1 × 10-4 μg/mL SVHRP application, respectively (P<0.01,n=8). The AP threshold was increased from (-41.17±2.15) mV to (-32.40±1.48) mV after 1×10-4 μg/mL SVHRP treatment (P<0.01,n=8). The peak amplitude of AP was (68.49±2.33) mV for the neurons before treatment with 1×10-4 μg/mL SVHRP and (54.71±0.81)mV after treatment (P<0.01, n=8). These results showed that SVHRP could decrease the AP firing frequency, increase the AP rheobase and threshold, but decrease the AP peak amplitude of hippocampal neurons.

在产生位相放电的45个细胞中,有8个细胞在SVHRP处理后仍可以诱发出位相放电(占17.78﹪);37个细胞在SVHRP处理后无法诱导出位相放电(占82.22﹪),SVHRP处理后动作电位的产生与处理前相比,有显著差异(P<0.01,n=45);在产生重复放电的7个细胞中,在1×10-2μg/mL SVHRP作用后均不能再次诱发出重复放电,而是产生一个动作电位或不再产生动作电位,药物处理前产生的动作电位个数为14.57±1.00,SVHRP处理后产生动作电位的个数为0.57±0.20,二者之间有显著性差异(P<0.01,n=7)。1×10-4 μg/mLSVHRP处理后,诱发动作电位产生的基强度由(75.10±8.99)pA增加到(119.85±12.73)pA(P<0.01,n=8);阈电位由(-41.17±2.15)mV升至(-32.40±1.48)mV(P<0.01,n=8);动作电位峰值由(68.49±2.33)mV下降至(54.71±0.81)mV(P<0.01,n=8)。

Being among them advertised for a ground of farmer society to help system is among thema foundation, being advertised for a ground of insurance system with social farmer is a core, is advertised for a ground of farmer to work society's guaranteeing system is the key that the whole system sets up.

其中被征地农民社会救助系统是基础,被征地农民社会保险系统是核心,被征地农民就业社会保障系统是整个制度构建的关键。

The main differences among these three test methods were compared, and correlation of evaluation among them were also discussed. The test results showed that the difference of test results is between 0.5 and 1.0 grade, and AATCC test method for wet crocking test showed more color transfer than the other two test methods.

结果表明,对于纺织品耐摩擦色牢度,三种测试方法所测得的耐摩擦色牢度值之间存在着一定的差异,虽然只有0.5~1.0级之差,但对于耐摩擦色牢度是否合格是至关重要的,AATCC湿摩擦的测试结果比其他两种稍显严重。

Straw checkerboard comprise of power system, portrait straw infixing system and landscape orientated straw infixing system. Among them, landscape orientated straw infixing system is the most difficult part in this project, which contains straw transporting、 straw exchanging、 cutting、 straw infixing、 automatic lift and needs high consistency and balance among these systems.

草方格铺设机器人主要由动力部分、纵向插草机构、横向插草机构等机构组成,其中横向部分是整个项目的难点,它由输草、换草、剪切、插草、自动起降等机构组成,机构之间工作要求具有高度的一致性和协调性。

Among them the most urgent ones, which will deeply effect the development of E-government, are interknitting among different operational systems, information sharing and operational cooperation.

各业务系统间的互联互通、信息共享、业务协同是电子政务深入发展最迫切需要解决的问题。

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推荐网络例句

Liapunov—Schmidt method is one of the most important method in the bifurcation theory.

Liapunov—Schmidt方法是分叉理论的最重要方法之一。

Be courteous -- even when people are most discourteous to you .

要有礼貌──即使当別人对你最不礼貌的时候。

I think we have to be very careful in answering these questions, because nothing is really so simple.

我认为,我们在回答这些问题的时候应该非常谨慎,因为事情远没有那么简单。