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Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon ( Pf ., Tg ., Tv .) and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点,与华支睾吸虫LDH同源性最高为75%,与阴道毛滴虫LDH同源性最低为17%,与人LDH(HsLDH-A,-B,-C)的同源性为58%~60%; SjLDH与果蝇的进化距离较秀丽隐杆线虫为近,3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近;该蛋白具有3个跨膜区域,3个高亲水性区域,主要的线性表位98aa~106aa位于膜外,与原虫LDH相同区域差异显著,而与其他LDH有1~3个氨基酸的差异,关键催化位点之一及底物丙酮酸结合区域位于该区域,蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构,3个关键催化位点位于该区域或在其附近。

He may find instead that he has bought himself more domestic difficulty by awakening the protectionist fantasies of a legion of other industries; and dashed hopes among the developing world that his administration is not simply a retread of other, politically myopic US administrations of years past.

但最终他会发现,此举只会引发众多其它行业的保护主义幻想,从而让自己陷入更多的国内麻烦。此举还粉碎了发展中国家的希望:它们发现,奥巴马政府不过是多年前政治短视的美国政府的翻版。

The network itself, freed of the control of broadcasters and other bandwidth owners, becomes the locus of a new system of distribution, based on association among peers without hierarchical control, which replaces the coercive system of distribution for all music, video, and other soft goods.

网络本身也摆脱了播音员和其他频带所有者的控制,为一种新的分配体系提供了场所;不存在层级控制的友侪间的合作,取代了音乐、影像等软产品的强制分配体系,成为这种新体系的基础。

In this study, the cDNA fragments of G-L intergenic region and polymerase activity module in L gene of two street rabies virus were cloned and sequenced. The nucleotides sequence of these cDNA fragments mentioned above and their deduced amino acid sequence were compared with that of rabies virus strains, rabies-related virus strains and other Rliabdoviridae published previously. The result show that the homology of nucleotide sequence of G-L IGR among MRV, DRV is 83.3%, compared with other rabies-related virus strains, the homology of G-L IGR is between 58.3%-91.7%. the length of G-L IGR of rabies virus is different in Rhabdoviridae.

本实验对狂犬病病毒野毒株MRV、DRV的G-L间隔区以及L基因聚合酶活性部位进行了克隆和测序,将所测定的结果和推定的氨基酸序列与国内外公开发表的狂犬病病毒其它固定毒株、野毒株和同科不同属的病毒的相应部分进行了分析比较,结果表明MRV、DRV G-L IGR核苷酸序列的同源性为83.3%,与其它毒株的同源性在58.3%~91.7%之间;狂犬病毒G-L间隔区序列的长度在弹状病毒科不同病毒属之间有所不同。

There are two kinds of spinels in a granulite terrain of Yushujgou, South Tianshan Mountain. One occurs in the intermediate and basic granulite units and is characterized by enriched Al and poor Cr, belonging to Al_spinel. In thin sections it shows dark green color and subhedral to anhedral form, and coexists with other metamorphic minerals, suggesting a neogenic metamorphic mineral of granulite facies. The other occurs in the adjacent ultramafic rock unit, belonging to Cr_spinel. In thin sections, it is dark brown in color and is scattered among olivine, clinopyroxene and orthopyroxene as irregular grains, indicating a stable residual mineral in the process of granulite facies metamorphism.

新疆榆树沟麻粒岩地体中发育两类尖晶石:一类见于中、基性麻粒岩体中,其化学成分富 Al贫 Cr ,属铝尖晶石,镜下为深绿色,呈半自形至他形粒状,与其它变质矿物共生,表现为麻粒岩相的新生变质矿物;另一类发育在空间上与麻粒岩体紧密相邻的超镁铁质岩体中,为铬尖晶石,镜下为深褐红色,呈不规则粒状分布于橄榄石、斜方辉石和单斜辉石之间,为麻粒岩相变质过程中稳定的残余矿物。

Of the tested plants had the same fragment at all stages of PMCs and in somatic cells, but other 44% had not, among them, however, 0.76% of their anthers produced the same specific fragment only at synizesis stage, but not at other stages or in somatic cells.

在这一片段中设计特异引物再次对兰州百合DNA进行检测,56%的个体整体存在B1片段;在剩余的44%个体中得到了0.76%的花药特异片段检出率,时期为凝线期。

Still, the belief in telegony persists among some politically marginal groups, such as White supremacists in the Western world, as well as some other racists and/or religious fundamentalists, e. g., in Russia and other ex-USSR countries.

然而,对先父遗传的信奉仍然存在於一些政治边缘团体中,如西方的白人至上主义,以及其他一些,比如存在於俄罗斯和其他前苏联国家中的种族主义和原教旨主义。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示:先证者尿检呈强阳性;其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变,为半合子突变,而其母、其姐为杂合突变;正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变;不同物种氨基酸序列的同源性比对显示: c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守;酶活性测定的结果显示:先证者的IDS酶活性仅为2.3 nmol/4 h/mL,大大低于正常值,而其父的为641.9 nmol/4 h/mL,其母的血浆酶活性为95.8 nmol/ 4h/mL,其姐的为103.2 nmol/4 h/mL。

Where two or more policies are effected by or on behalf of the assured on the same adventure and interest or any part thereof, and the sums insured exceed the indemnity allowed by this Act, the assured is said to be over-insured by double insurance (2) Where the assured is over-insured by double insurance The assured, unless the policy otherwise provides, may claim payment from the insurers in such order as he may think fit, provided that he is not entitled to receive any sum in excess of the indemnity allowed by this Act; Where the policy under which the assured claims is a valued policy, the assured must give credit as against the valuation for any sum received by him under any other policy without regard to the actual value of the subject-matter insured; Where the policy under which the assured claims is an unvalued policy he must give credit, as against the full insurable value, for any sum received by him under any other policy; Where the assured receives any sum in excess of the indemnity allowed by this Act, he is deemed to hold such sum in trust for the insurers according to their right of contribution among themselves.

当被保险人或其代表,(1)就同一冒险和利益或其中的一部分订立了两份以上的保险单,且保险金额超过本法所允许的赔偿限额时,被保险人即被视为因重复保险而超额保险。(2)在被保险人因重复保险而超额保险之场合:除非保险单另有规定,被保险人可根据自已认为合适的顺序,依次向其保险人索赔,但他所得金额不得超过本法允许的赔偿限额;如果被保险人凭以索赔的保险单是一份定值保险单,被保险人必须将其他保险单项下他已收取的任何数额从约定保险价值中扣除,无需考虑保险标的的实际价值;如果被保险人据以索赔的保险单是一份不定值保险单,被保险人必须将其他保险单项下他已收取的数额从全部保险价值中扣除;若被保险人得到的金额超过本法所允许的赔偿额,则此种超出金额即被视为由被保险人代各保险人托管,由保险人按他们之间的分摊权利摊回。

Where two or more policies are effected by or on behalf o f the assured on the same adventure and interest or any part thereof, and the sums insured exceed the indemnity allowed by this Act, the assured is said to be over-ins ured by double insurance (2)Where the assured is over-insured by double insurance The assured, unless the policy otherwise provides, may claim payment from the insurers in such order as he may think fit, provided that he is n ot entitled to receive any sum in excess of the indemnity allowed by this Act;Where the policy under which the assured claims is a valued policy, the assured must give credit as against the valuation for any sum rec eived by him under any other policy without regard to the actual value of the subject-ma tter insured;Where the policy under which the assured claims is an unvalu ed policy he must give credit, as against the full insurable value, for any sum rec eived by him under any other policy; Where the assured receives any sum in excess of the indemnit y allowed by this Act, he is deemed to hold such sum in trust for the insurers acco rding to their right of contribution among themselves.

1当被保险人或其代表,就同一冒险和利益或其中的一部分订立了两 32。份以上的保险单,且保险金额超过本法所允许的赔偿限额时,被保险人即被视为因重复保险而超额保险。(2)在被保险人因重复保险而超额保险之场合:除非保险单另有规定,被保险人可根据自己认为合适的顺序,依次向其保险人索赔,但他所得金额不得超过本法允许的赔偿限额;如果被保险人凭以索赔的保险单是一份定值保险单,被保险人必须将其他保险单项下他已收取的任何数额从约定保险价值中扣除,无需考虑保险标的的实际价值;如果被保险人据以索赔的保险单是一份不定值保险单,被保险人必须将其他保险单项下他已收取的数额从全部保险价值中扣除;若被保险人得到的金额超过本法所允许的赔偿额,则此种超出金额即被视为由被保险人代各保险人托管,由保险人按他们之间的分摊权利摊回。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

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