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Total RNA was extracted from flowers of cattleya, according to the conserved acid sequence for ACC oxidase in other orchids, we designed a pair of oligo nucleotide primers. Using RT-PCR method, a cDNA fragment about 967 base pair, which encoded 321 predicted amino acid residues, was amplified.

以卡特兰花瓣为试材，提取其总RNA，并根据其他兰花的ACC氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase， ACO)基因保守序列设计一对特异性引物，通过RT-PCR法克隆得到1条967bp的卡特兰ACO cDNA片断，共编码321个氨基酸残基。

PhrIP1(phragmoplastin-interacting proteint l),a protein of 608 amino acids,appears to play an important role in the formation of cell plate during cytokinesis in plants.

摘要成膜素相关蛋白1(PhrIP1)是一个含608个氨基酸的蛋白质，它对于植物胞质分裂中细胞板的形成起到了十分重要的作用。

The research on placenta through modern science show that its important ingredient is immuneglobulin and sneeiyne, which is the anti-aging gene, enzyme, which has high appliance value, and interferon, which protein can reach more than 80%, amino has rich resources、full ranges、and reasonable ration, which fat is less than 10%, and also has rich lecithin, cephalin, lipopolysaccharide, a variety of vitamins and trace elements,in addition, it has transfer factor, protein active factor, collagen element, organic phosphorus compound, amniotic acid and nucleic acid compound and so on.

采用现代科学对羊胎素进行分解，发现其重要成份是免疫球蛋白和延缓衰老基因sneeiyne具有很高应用价值的酶和干扰素，其中蛋白质的含量在80%以上，氨基酸含量丰富，种类齐全，配比合理，脂肪含量小于10%，还含有丰富的卵磷脂，脑磷脂，脂多糖，多种维生素和微量元素，此外，还有转移因子，蛋白质活性因子，胶原蛋白素，有机磷复合物，羊膜酸和核酸化合物等。

Immunoglobulin and anti-aging gene have a high value sneeiyne.sneeiyne enzyme and dry IFN-, in which the protein content of more than 80%, amino acid-rich.full range, areasonable proportion, Less than 10% aft content, but also rich in, cephalin, LPS, a variety of vitamin and trace element Su-, in addition to transfer factor, protein activating factor, collagen Su, organic phosphorus compounds, membrane acid And nucieic acids and other compounds.

免疫球蛋白和延缓衰老基因sneeiyne.sneeiyne是具有很高应用价值的酶和干扰素，其中蛋白质的含量在80%以上，氨基酸含量丰富，种类齐全，配比合理，脂肪含量小于10%，还含有丰富的卵磷脂，脑磷脂，脂多糖，多种维生素和微量元素，此外，还有转移因子，蛋白质活性因子，胶原蛋白素，有机磷复合物，羊膜酸和核酸化合物等。

The research results indicated that the best base was KOH, the best acid was H3PO4 and their neutralizing products were the necessary nutrients for foliar fertilizer; 12.4 L of foliar fertilizer were obtained in per kg of shrimp offal, chitin was obtained in 13.8% yield, crude protein was obtained in 23.2% yield using this method; This multifuctional foliar fertilizer was an abundant nutritional multifunctional foliar fertilizer which contained 15 kinds of amino acids, shimp cephalin, astacein and many trace elements. The concentration of calcium was 38.9 mg/L, the concentration of magnesium was 55.4 mg/L, the concentration of potassium was 20.0 g/L, the concentration of nitrogen was 4.5 g/L and the concentration of phosphorus was

研究结果表明：(1)处理虾下脚料的最佳碱是KOH，最佳酸是H3PO4，其中和产物是叶面肥必需的营养素；(2)本工艺不仅每Kg虾下脚料可制备虾叶面肥12.4L，而且可回收13.8％甲壳素和23.2％粗蛋白质；(3)经分析测定虾叶面肥中，含钙(Ca2+)38.9 mg/L，含镁(Mg2+)55.4 mg/L，含钾20.0 g/L，含氮4.5 g/L，含磷 78.9 g/L，还含有15种氨基酸及虾脑磷脂、虾红素及多种微量元素等，是一种营养丰富的多功能叶面肥。

Objectives To study the change of excitatory amino acids glutamate and aspartic in cerebrospinal fluidof children with bacterial meningitis and their function in pathophysiological mechanisms and clinical significance.

目的 观察细菌性脑膜炎患儿脑脊液中兴奋性氨基酸谷氨酸和天门冬氨酸含量的变化，并探讨其在细菌性脑膜炎病理生理过程中的作用和临床意义。

The second structure and the amino acid sequence of TasA protein were predicted by using the softwarerhttp://npsa-pbil.ibcp.fr/cgi-bin/secpred mlr.pl.

利用http：//npsa-pbil.ibcp.fr/cgi.bin/secpred_mlr.pl在线分析预测了TasA基因的氨基酸序列和二级结构特征。

Moreover, the size of the molecule and steric hindrance showed obvious effects on the interaction, while the configuration, sequence and length of carbon chains (increasing 1～2 C) of amino acid residue in the phosphoryl oligopeptides showed little effects on the interaction under ESI-MS conditions.

磷酰化二肽中氨基酸残基的构型、顺序、碳链长短的变化(增加1～2个C)对其与蛋白溶菌酶之间的相互作用在质谱中没有表现出影响。

Using Cassia tora as material,a cDNA encoding chalone synthase was cloned from tender leaves of Cassia tora by RT-PCR and RACE.The full-length cDNA of CHS from Cassia tora had 1 459 bp with an open reading frame encoding 390 amino acids of protein.

以决明为实验材料，利用RT-PCR和RACE技术，从决明嫩叶中克隆出查尔酮合成酶(Chal-one synthase，CHS)基因，其cDNA全长为1 459 bp，编码一个由390个氨基酸残基组成的多肽。

Four experiments were conducted to study the nutritional function mechanism andrelative biological availability of iron amino acid chelate.

随着集约化养殖的迅猛发展和人们环境保护意识的逐渐增强，作为第三代铁添加剂的主要代表氨基酸螯合铁也越来越受到营养学家的关注。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新