查询词典 amino-azo-benzene

There are 20 kinds of amino acid, among them, 10 kinds are necessities which can not be synthesized by animal bodies, and must be obtained from fodders; lysine is the most important in the 10 kinds of amino acid.

氨基酸有20种，有十种为必须氨基酸，动物体内不能自行合成，需从饲料中获得，赖氨酸就是其中最重要的一种。

Following this discovery, a variety of neurotoxins were characterized and their amino acids sequences, secondary structure and tertiary structure were also characterized. These toxins are polypeptides of single chain and consist of 46-49 amino acids, cross-linked by three disulfide bridges. The ordered secondary structure has been determined on the basis of NMR studies. The dominant feature is a four-stranded anti-parallel β-sheet, linked with three loop.

随后，又有多种海葵毒素被发现，并做了氨基酸序列测定，以及二级、三级结构的分析，从已报道的海葵神经毒素的一级结构来看，大都是由46-49个氨基酸组成的单链多肽，分子量5kD左右，有6个cys残基，形成3对二硫键，它们的二级结构中不存在α螺旋结构，而是4链反向β折叠，β折叠由3个大小不同的环结构相连。

The full-length cdna of bin2a is 2606bp, encoding 637 amino acid. its amino acid sequence has nine conserved blocks of glycosyl hydrolases family 35, including an active site glu210 of proton donor, but lacking another active site glu279 of nucleophile.

bin2a的全长cdna为2606bp，编码×××个氨基酸。bin2a的氨基酸序列中包括glycosyl hydrolases family ×××家族中的9段相对保守的序列，其中包括210位的活性中心glu，但是缺少另外一个活性中心，279位的glu。

Preparation of compound amino acids from dehulled double - low rapeseed meal hydrolysed by sul-furic acid was studied . We attempted to produce amino acids by a two - step neutralization.

为得到高质量的菜籽复合氨基酸，以脱皮菜籽粕为原料，研究了硫酸水解制备复合氨基酸的新工艺一二次中和法，解决了氨基酸脱色和副产品植酸的利用问题。

In order to study the effect of PPh_2 group in the application of chiral ligands 4, 5, 7 and 8 to asymmetric reactions, two ferrocenyl amino alcohols 9a and 9b were Scheme 3 synthesized from chiral amino alcohol derived imines 3 (Scheme 3) and applied in the same asymmetric reactions.

为了探讨二苯氧膦基在配体参与不对称催化反应时对其催化活性和选择性的影响，合成了两个手性二茂铁基氨基醇配体9a和9b(Scheme 3)。

In the fifth part, asymmetric synthesis of β,γ-trans-alkenyl α-amino esters was achieved by chiral phosphoric acid catalyzed transfer hydrogenation of β,γ-alkynyl α-imino esters. Utilizing Hantzsch ester as the hydrogen donor, both the alkyne and imine moieties of β,γ-alkynyl α-imino esters were reduced to yield β,γ-trans-alkenyl α-amino esters with up to 96% ee.

第五部分工作中，使用手性磷酸作为催化剂，Hantzsch酯作为氢源，β，γ-炔烃取代的α-酮酸酯亚胺中的炔键和亚胺官能团均被还原，以中等的收率和优秀的对映选择性（up to 96% ee）得到β，γ-反式烯烃取代的α-氨基酸衍生物。

This research take late-maturing variety adzuki bean Jihong-4 as the experiment material, After the photoperiod inducement at different leaf ages treatment, the result showed that the activity of NR under LF treatment is higher than CK treatment on the whole, and is lower than SD treatment; The content of the dissociation amino acid under these three treatments show a descend trend along with the leaf ages accretion, but the inducement increasing the content of dissociation amino acid, different leaf ages leaves show some difference, and alternating rise with SD treatment; Inducement can reduce the content of soluble sugar, and continuous inducement shows a accumulation effect; 2LF is the most sensitivity stage for soluble protein under photoperiodic inducement, From this stage, each leaf indumenta can reducing the content soluble protein, but continuous inducement reducing rising it.

本研究通过光周期诱导的方法，对中晚熟品种冀红4号红小豆进行不同叶龄进行处理，结果表明：所有叶龄处理下硝酸还原酶活性基本高于自然光处理而低于连续短日照处理下的；随着叶龄增大，各处理游离氨基酸总量均呈下降趋势，但光周期诱导可提高游离氨基酸含量，不同叶龄表现有所差异，与连续诱导交替上升；诱导可降低可溶性糖的含量，并连续诱导出现累积效应；2LF是光周期诱对可溶性蛋变化最敏感的时期，这个时期起单个叶龄诱导可降低可溶性蛋白含量，但连续诱导却可提高；LF处理下的类胡萝素总体含量高于另两个处理，而LF处理下的叶绿素含量在仅3叶龄前高于CK处理下的，随后出现下降趋势。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

And the complete cDNA sequence of GSTR was 1002bp in length, containing a coding region of 678bp (encoding 225 amino acids). Results of the phylogenetic analysis revealed that the deduced amino acid sequence of the orange-spotted grouper was very similar to the GSTA of other fishes, such as common carp and zebrafish. The GSTR existed only in fish, but not in any other aquatic animals or land mammals (e.g. rat, mouse and human).

构建系统进化树发现，斜带石斑鱼与鲤鱼、斑马鱼GSTA氨基酸同源性较高，而GSTR为鱼类所特有并共同占据进化树上独立的分枝，与大鼠、小鼠、人等哺乳动物GST现有所有类型同源性均较低。

Hydrolysis of the mischarged amino acids in theediting reaction will rely on the proper binding and correct recognition of both mainchain and side chain of the substrate. In general, the editing active site ofLeuRS-ED has a very rigid structure with a defined stereochemistry and usesa number of conserved residues to precisely bind and recognize the misactivatedamino acids and discriminate and reject the cognate amino acid, specifically usingMet336, Asp345, and possibly Thr247 for main-chain recognition and Thr252,Met336, and Val338 for side-chain recognition.

在编校反应中错配的氨基酸的水解依赖于对底物主链和支链的适当的结合和正确的识别。ecLeuRS-ED的编校活性位点有一个非常刚性的有很精确设计的立体化学性质的结构，采用一系列保守氨基酸残基来精确结合和识别错配的非共轭氨基酸而区分排斥正确的共轭氨基酸，具体就是采用Met336、Asp345，另外可能还有Thr247对底物主链进行识别，采用Thr252、Met336和Val338对支链进行识别。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。