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Lactobacillus plantarum had lipolytic activity. Bacillus subtilis not only had the lipolysis but also had proteolysis.Single strains fermenting experiment showed that, the effect of different strain to the pH, acidity, the total amino acid were not signifient, Pediococcus pentosaceusand Lactobacillus plantarum could improve the content of free amino acid in dried beef.

研究结果表明:沃氏葡萄球菌、腐生葡萄球菌和植物乳杆菌只有脂肪酶活性,无蛋白酶活性;松鼠葡萄球具有弱蛋白酶活性;木糖葡萄球菌具有弱脂肪酶活性;戊糖片球菌具有脂肪酶活性,而蛋白酶活性较弱;枯草芽孢杆菌具有两种酶活性。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序,序列分析结果表明:该基因片断长为1266 bp,包括1031 bp的编码区序列,编码343个氨基酸;其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上,并构建了它们间的亲缘关系树。

The results were as follows the change of vitamin C content was a "trial apex"-shape curve, higher in young fruit,incrustation and maturation stages, lower in other periods; the total acid content was the highest in young fruit, after that, it decreased rapidly, maintained lower level, and reached lowest when the fruit matured; the total content of soluble sugar inosculated with the fruit development, it was high when the fruit development was quick, and low when the fruit development was slow; before 59 d after anthesis, the reducing sugar content was high in the total content of soluble sugar and after that ,the nonreducing sugar content was high; before 45 d after anthesis, free amino acid content was obviously correlated with protein content, and they inosculated with the fruit development;4559 d after anthesis, free amino acid content increased quickly, and protein content decreased gradually, this was also the most shaping period of the embryo and endosperm.

结果表明,Vc含量呈&三峰&型曲线,幼果时、硬核后和成熟时较高,其他时期较低;有机酸含量以幼果期最高,以后迅速下降并维持较低水平,果实成熟时含量最低;可溶性总糖含量与果实发育状况基本相吻合,果实发育速度快时含量较高,慢时较低;在谢花后59 d以前,可溶性糖以还原糖为主,之后以非还原糖为主;游离氨基酸和蛋白质含量,在谢花后45 d以前两者呈高度相关,且与果实生长速度相吻合,谢花后4559 d是胚和胚乳的主要形成期,此期游离氨基酸含量迅速增加,蛋白质含量持续下降。西北林学院学报21卷第5期张传来等金光杏梅果实发育期间主要营养成分动态变化研究

The contents of lysine, alanine and arginine among 17 kinds of amino acids had been identified to be opposite correlation with the leaf damage index (r=-0.9389, P.05; r=-0.8925, P.05; r=-0.8905, P.05) respectively. But nymphal density of Erythroneura apicalis Nawa had no significant relationship with 17 kinds of amino acids.

在17种氨基酸中,葡萄斑叶蝉的虫口密度与各氨基酸的含量无显著相关关系,但叶片被害指数与赖氨酸、丙氨酸、精氨酸的含量呈负相关关系(r=-0.9389, P.05; r=-0.8925, P.05; r=-0.8905, P.05)。

Kb, the cDNA length is 6022 bases, the PPO amino acid sequence has the genes of the open-reading frame of 993 amino acid residues, the gene is expressed in multiple tissues of human cancer, including oophoron cancer, mammary cancer, pancreas adenocarcinoma, colon cancer, lung carcinoma and prostate cancer.

PPO的氨基酸序列具有编码SEQ ID NO.2所示的993个氨基酸残基的可读框的基因。该基因在人类多种癌组织中(卵巢癌,乳腺癌,胰腺癌,结肠癌,肺癌,前列腺癌,脑癌,胃癌,肝癌,子宫癌等)都有过表达。活性成份可制备成癌症诊断剂或试剂盒。

The synthesis method of the invention is as follows: first, 5-amino-2-(p-aminophenyl)- benzoxazole and aliphatic diacid are respectively recrystallized; NMP is distilled; solubilizer is dried; second, in an atmosphere of nitrogen, after Py and NMP are mixed evenly and added to a sealed reactor, the solubilizer is added into the reactor, followed by the 5-amino-2-(p-aminophenyl)- benzoxazole, then the mixture in the reactor is stirred; second, in an atmosphere of nitrogen, the mixture in the second step is stirred and heated to 50-150 DEG C, then the aliphatic diacid is added, followed by TPP for condensation; fourth, after methanol is poured into the reactor, the sediment is separated out from the mixture, filtered, washed and dried, so the semi-aromatic polyamide containing oxazole ring is obtained.

本发明的合成方法如下:一、分别重结晶5-氨基-2-苯并唑与脂肪族二酸;蒸馏NMP;干燥增溶剂;二、在氮气气氛下,Py与NMP混匀后加入密封的反应器中,再加入增溶剂,然后加入5-氨基-2-苯并唑,搅拌;三、在氮气气氛下,将步骤二的混合液搅拌并升温至50℃~150℃后加入脂肪族二酸,再加入TPP后缩聚;四、再倾入甲醇析出沉淀,过滤,清洗沉淀,再干燥;即得到含唑环半芳香聚酰胺。

The content of essential amino acid and flavor amino acid of experiment groups were higher than control group, especially group 1.0% small peptide were increased by 6.4% and 2.5%; IMP content of experiment groups was increased in different extent, especially group 1.0% small peptide was 0.17mg/100mg and increased by 70.0%(P.05); The content of palmitoleic acid、 octadeca、 arachidonic、 arachidic and stearic acid of experiment groups were higher than control group indifferent extent.

随着小肽用量的增加,成鲤肌肉中的总氨基酸含量呈上升趋势,必须氨基酸和风味氨基酸的含量也随之提高,尤其是1.0%小肽组的必须氨基酸和风味氨基酸的含量分别比对照组提高6.4%和2.5%,其中谷氨酸的含量最高,为10.70mg/100mg,比对照组提高14.0%(P<0.05);随着饵料中小肽用量的增加,成鲤肌肉的肌苷酸含量显著提高(P<0.05),1.0%小肽组的IMP含量高达0.17mg/mg,比对照组提高70.0%;成鲤肌肉中的油酸和二十?

Become the pharmacodynamical functional group to realize the direct connection on body's endo-sugar with amino acid, and then to make the function of discharging lead after sugar taking amino acid into cell to complex with lead.

本论文选择葡萄糖和阿拉伯糖作为药动团,L-半胱氨酸等其它侧链氨基酸作为药效团,实现人体内源性糖和氨基酸的直接相连,从而发挥糖携带氨基酸进入细胞螯合排铅的作用。

The results showed that the gene and amino acid sequence homology between seven species are over 44.3% and 47.90/o, respectively. In addition, conservation of three sequences: EQCGSQAGGALCP, CCSQFOWCGST and CQSQC in the 40 amino acids sequence at the beginning of the N-terminal chitinase mature protein is very high, The conservation of eight Cys at the sites of 3,12,17,18,24,31,36,40 is 100% Finally, the chitinase signal peptide gene from Phaseolus vu?

结果表明七种基因之间有44.3%以上的同源性,蛋白之间有47.9%以上的同源性,而且在成熟蛋白N端前40个氨基酸序列中分别位于1~13、17~27和36~40位的EQCGSQAGGALCP、CCSQFGWCGST和CQSQC序列区保守性很高,尤其是位于第3、12、17、18、24、31、36、40位的八个半胱氨酸的保守性达到100%。

The international nutrient value assessment method was applied to the overall assessment of the protein nutrient value of Pholiota adiposa mycelia. The assessing result showed that the total essential amino acid in Pholiota adiposa mycelia was very high, making up to about 40.0% of the total amino acids.

相对黄伞子实体的栽培而言,通过液体发酵技术生产黄伞菌丝体具有产量高、周期短、不受气候变化的影响、条件易于控制等优点,因此,对黄伞菌丝的液体发酵工艺和菌丝体的营养价值进行研究具有重要的实际意义。

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