查询词典 amino acid

The nucleotides sequence and deduced amino acid sequence of these cDNA figments mentioned above were divided into two fractions. One is carbon part of L gene , the other is 5 terminal,and they were compared with other rabies-related virus strains published in NCBI previously.The result show that the homology of nucleotide sequence and amino acid sequence of carbon part in L gene among these two rabies virus strains is 91.3% and 95.8%, and these two strains belong to type of gene I.

本实验以吉林鹿源DRV8202株及河南鼠源野毒株MRV的L基因的C端为主要研究内容，用RT-PCR和3'-RACE方法对其进行了克隆和测序，将所测得的核苷酸序列和推导的氨基酸序列分成两个部分，一是L基因C端序列，另一个是L基因5'端的非编码区，并与NCBI上公开发表的狂犬病毒其它毒株的相应片段进行分析比较。

There are 20 kinds of amino acid, among them, 10 kinds are necessities which can not be synthesized by animal bodies, and must be obtained from fodders; lysine is the most important in the 10 kinds of amino acid.

氨基酸有20种，有十种为必须氨基酸，动物体内不能自行合成，需从饲料中获得，赖氨酸就是其中最重要的一种。

The full-length cdna of bin2a is 2606bp, encoding 637 amino acid. its amino acid sequence has nine conserved blocks of glycosyl hydrolases family 35, including an active site glu210 of proton donor, but lacking another active site glu279 of nucleophile.

bin2a的全长cdna为2606bp，编码×××个氨基酸。bin2a的氨基酸序列中包括glycosyl hydrolases family ×××家族中的9段相对保守的序列，其中包括210位的活性中心glu，但是缺少另外一个活性中心，279位的glu。

This research take late-maturing variety adzuki bean Jihong-4 as the experiment material, After the photoperiod inducement at different leaf ages treatment, the result showed that the activity of NR under LF treatment is higher than CK treatment on the whole, and is lower than SD treatment; The content of the dissociation amino acid under these three treatments show a descend trend along with the leaf ages accretion, but the inducement increasing the content of dissociation amino acid, different leaf ages leaves show some difference, and alternating rise with SD treatment; Inducement can reduce the content of soluble sugar, and continuous inducement shows a accumulation effect; 2LF is the most sensitivity stage for soluble protein under photoperiodic inducement, From this stage, each leaf indumenta can reducing the content soluble protein, but continuous inducement reducing rising it.

本研究通过光周期诱导的方法，对中晚熟品种冀红4号红小豆进行不同叶龄进行处理，结果表明：所有叶龄处理下硝酸还原酶活性基本高于自然光处理而低于连续短日照处理下的；随着叶龄增大，各处理游离氨基酸总量均呈下降趋势，但光周期诱导可提高游离氨基酸含量，不同叶龄表现有所差异，与连续诱导交替上升；诱导可降低可溶性糖的含量，并连续诱导出现累积效应；2LF是光周期诱对可溶性蛋变化最敏感的时期，这个时期起单个叶龄诱导可降低可溶性蛋白含量，但连续诱导却可提高；LF处理下的类胡萝素总体含量高于另两个处理，而LF处理下的叶绿素含量在仅3叶龄前高于CK处理下的，随后出现下降趋势。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

With the aid of 〓P NMR examination for the process and ESI-MS/MS analysis of the derivatized products from the reaction intermediate, it is found that the corresponding phosphoryl chloride is generated after mixing the hydrogen phosphonate derivative with chlorodiisopropylamine, and they react with amino acid, amino acid methyl ester, dipeptide methyl ester or N-methyl imidazole carboxyl alkyl diamine respectively to form the desired products.

利用〓P NMR对反应过程监测以及对反应中间体衍生化后产物的ESI-MS／MS检测分析发现，氢亚磷酸二酯与氯代二异丙胺反应首先生成了磷酰氯中间产物，它继续与氨基酸、氨基酸甲酯、二肽甲酯、1-甲基咪唑-2-酰基烷基二胺等反应生成了目标产物。

The pectin esterase gene and amino acid permease gene abundantly expressed in floral buds of NCa CMS while pectin esterase gene showed no transcript either in young leaves of NCa CMS or in the two tissues of maintainer line, and amino acid permease gene slightly expressed in the corresponding tissues.

结果表明，果胶甲酯酶基因和氨基酸通透酶基因都在不育系花蕾中大量表达，果胶甲酯酶基因在不育系叶片、保持系的花蕾、叶片中都没有检测到；而氨基酸通透酶基因有微量表达。

According to amino acid compositions and pseudo amino acid composition, the membrane protein types were predicted by using supervised locally linear embedding algorithm.

将氨基酸的组成含量以及它们之间的相关系数作为膜蛋白序列的特征向量，采用有监督的局部线性映射的方法对该向量进行降维，并使用最简单的欧氏距离分类器来预侧膜蛋白类型。

Based on the amino-acid sequence in protein, eight types of membrane proteins were predicted by using the algorithm of random forests with the increment of diversity and the pseudo amino acid composition information.

文章基于蛋白质的氨基酸序列，将组合离散增量和伪氨基酸组分信息共同作为预测参数，采用随机森林分类器，对8类膜蛋白进行了预测。

The total content of amino acid and sulphur -amino acid in grain of cultivars with HMW-GS composition both 2+12 and 5+10 were increased by sulphur application under the condition of high nitrogen level, and the positive c

总氨基酸含量和含硫氨基酸含量高可能是NE7742表现优质的一个原因。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48