查询词典 amidase

The thermal inactivation of penicillin amidase entrapped in AOT/isooctane reversed micelles was studied in detail.

研究了青霉素酰化酶在二-(2-乙基己基)琥珀酸双酯磺酸钠/异辛烷反胶团中的稳定特性。

It has been shown that penicillin amidase was very susceptible to the change of temperature, extremely apt to deactivate in the buffer; while the thermostability of enzyme could be improved by an average of 2～4 times in the reversed micelles.

结果表明，青霉素酰化酶对温度非常敏感，在水溶液中极易失活，而经反胶团包埋后，其热稳定性有很大程度的提高，热失活明显减少。

The hydrolysis course of PenG catalyzed by penicillin amidase entrapped in the AOT/isooctane reversed micelles was investigated.

通过将青霉素酰化酶包埋于AOT/异辛烷反胶团中，对PenG酶促水解过程进行了研究。

The K m and V max values of the amidase for acrylamide were 2.64 mmol·L-1 and 0.6 μmol·min-1·ml-1, respectively.

以丙烯酰胺为底物时，该酶的 K m和 V max值分别为2.64 mmol·L-1和0.6 μmol·min-1·ml-1。

At the same temperature, k〓 in the reversed micelles was always less than k＇〓 in the buffer. According to the thermodynamic analysis for inactivation of penicillin amidase in the reversed micelles, the free energy of activation was increased by 2. 71%, while the entropies and enthalpies of activation were decreased, corresponding to 63. 9% and 76. 9% of that in pure buffer respectively.

由热力学分析可知，青霉素酰化酶在AOT/异辛烷反胶团中的热失活过程，与水溶液中相比，活化自由能△G〓增加了2.71％，而活化熵△S〓和活化焓△H〓分别减小为原来的63.9％、76.9％，从而使酶热稳定性平均提高了2～4倍。

The deactivation rates of penicillin amidase in both systems follow the first-order model. The inactivation rate constants increased gradually and α decreased slightly, with the increase of temperature.

随着温度的升高，失活速率常数k〓和k＇〓逐渐增大，α略有所降低；当体系温度相同时，该酶在反胶团中的k〓值比水溶液中的k＇〓小。

In this thesis, the dynamic course of penicillin G hydrolysis to 6-aminopenicillanic acid (6-APA) with penicillin amidase entrapped in the bis (2-ethylhexyl) sulfosuccinate /isooctane reversed micelles was studied.

本文主要研究青霉素酰化酶在反胶团体系中催化青霉素G水解的反应过程，并对反胶团介质中酶的稳定特性、反应动力学以及反应器开发等方面进行了较为系统的研究。

Most of these peptides are amidated at their C-terminals, which is essential for their full activities. The a-amide comes from posttranslational modification that is catalyzed by α-amidase (α-AE or PAM). The amidation reaction is a rate-limiting step in the biosynthesis of regulatory peptides.

大部分活性肽的C-端是酰胺化的，而且α-酰胺基为多肽的完全生物活性所必需；α-酰胺基来自多肽的翻译后加工，由α-酰胺化酶催化完成，是酰胺化多肽生物合成途径中的限述步骤。

With penicillin amidase entrapped in AOT/isooctane reversed micelles.

根据合理假设，建立了青霉素酰化酶在该耦合系统中水解PenG的反应动力学模型，与实验结果基本吻合。

Bacillus cereus;amidase;acrylamide;purification

蜡状芽孢杆菌；酰胺酶；丙烯酰胺；纯化

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。