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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

objective to evaluate the value of infrared thermography in the diagnosis of injuries in such soft tissues as neck,shoulder,waist,leg,elbow and wrist.methods infrared thermograph (type wp-95) was used to do general physical check up for 221 young soldiers with a regular military training history ranging from 1 to 5 years,whose infrared thermograms were then compared with those of 100 new recruits without any military training experience.results there were abnormal thermograms on 180 (81.45%) of the 221 cases and 6 (6%) of the 100 new recruits (p.05.all of the 186 cases with abnormal thermograms were x-rayed with only 28 cases(i.e.15.05%) abnormal.there was a distinct difference between the two methods of physical check-up (p.05).conclusion infrared thermography manifests distinctive abnormal thermograms of soft tissue injuries suffered from military training,providing definite diagnostic message for clinic.to utilize infrared thermography correctly is of vital importance in ensuring the health of servicemen.

目的 探讨软组织损伤颈、肩、腰、腿、肘、腕红外热像图的诊断价值。方法应用wp-95型红外热像仪,对221例常规军事训练1~5年的年轻干部及战士进行红外热像仪的全身检查,并与刚入伍未参加训练的新兵100例进行对照。结果 221例经红外热像图检查发现颈、肩、腰、肘、腕出现异常热像图的有180例,占81.45%;而100例新战士仅有6例颈、肩、腰、肘、腕出现异常热像图,占6.00%,两者比较差异有显著性(p<0.05)。186例异常热像图受检者全部做x线摄片检查,x线摄片异常者仅28例,占15.05%,两者检查比较差异有显著性(p.05)。结论红外热像图对军事训练所致软组织损伤有其独特的异常热像图,为临床提供明确的诊断信息,正确的使用红外热像图,对保证部队广大指战员的健康水平有着重要作用。

We want to move up ticklish Stone, the facilities of the moment will be greater than the force of gravity produced by a rock moment, we all know that the moment arm of force multiplied by the force equal to the size of the arm of force as an arm of force in the greater 2 , Finger pressure, the more gravity than, stone ticklish ticklish point of this very long arm of force, so we used a very small force so that the stones will be able to move up.

我们要想让怕痒石动起来,所施的力的力矩就要大于石头重力所产生的力矩,我们都知道力矩等于力臂乘以力的大小,当力臂2越大于力臂1时,手指压力也就越小于重力,怕痒石怕痒的这个点的力臂非常长,所以我们用很小的力就能使石头动起来。

In those years the households used to make bacon and sausage all by themselves. My family loved to make sauce-seasoned pork, that is, to have fresh pork pickled in salt and Chinese prickly ash for a week, and then to have it dried up in the open air and apply sweet sauce to it for several times, and finally dry it up for use.

那年月家家户户还有自制香肠腊肉的传统习惯,我们家每年也爱做酱肉,将买来的新鲜猪肉用盐、花椒等腌上一周,再晾在室外任风吹日晒,并一层层地往上面抹甜酱,待晾干后煮熟食用,味道特别香。

At present, English is most widely used all over the world; products must be sugared up before promotion. In order to reach the purpose of making our students learn professional knowledge and at the same time the ability to sugar up their products, we decide to implement the Hundred-product project.

目前,英语是世界最通用的语言,使得我们的产品必须用她进行包装才能走出去,为了让我们的学员在学到专业知识的同时也达到包装企业的目的,我们决定实施百事英语项目。

She leaned back far to look up where the fireworks were and she caught her knee in her hands so as not to fall back looking up and there was no-one to see only him and her when she revealed all her graceful beautifully shaped legs like that, supply soft and delicately rounded, and she seemed to hear the panting of his heart, his hoarse breathing, because she knew too about the passion of men like that, hotblooded, because Bertha Supple told her once in dead secret and made her swear she'd never about the gentleman lodger that was staying with them out of the Congested Districts Board that had pictures cut out of papers of those skirtdancers and highkickers and she said he used to do something not very nice that you could imagine sometimes in the bed.

除了他和她而外,没有一个人在看着,所以她把她那双俊秀而形态优美、娇嫩柔韧而细溜丰腴的小腿整个儿裸露出来。她似乎听到他那颗心的悸跳,粗声粗气的喘息,因为她也晓得像他那样血气方刚的男人,会有着怎样的情欲。还因为一次伯莎。萨波尔告诉过她一桩绝对的秘密,并要她发誓永远不说出去。她家的一位在人口密集地区调查局工作的房客,从报纸上剪下那些表演短裙舞和翘腿舞的舞女的照片。她说,他不时地在床上做些不大文雅的勾当,这,你也想象得到吧。

Bang Up-to-date and Easy to Use- References to over 170,000 words, phrases and examples explained in clear and natural English- All the important new words that have come into the language (e.g. dirty bomb, lairy, 9/11, clickable)- Over 200 Common Learner Error notes, based on the Cambridge Learner Corpus from Cambridge ESOL exams- Only words from a defining vocabulary used to help make definitions easy to understand- Over 90,000 examples, mainly full sentences, show you how English is used in context and help you write natural English.

邦截至到最新的和易于使用,引用超过17万单词,短语和例子解释清楚,自然的英语所有重要的新话已到语言,例如肮脏炸弹,lairy,9 / 11,可点击-超过200共同学习笔记错误的基础上,剑桥学习者语料库从剑桥ESOL考试,只有一个词从定义词汇用来帮助作出定义很容易理解,在90,000例子,主要是完整的句子,告诉你如何使用英语语境,并帮助您写英文。

objective to introduce a method for repair of defects of alae nasi.methods transplantation of whole or partial free auricle composite tissue was designed in reconstruction of alae nasi.partial auricle composite tissue transplantation was used in 14 patients,the area of the graft was 1.0cm×1.5cm~1.9cm×2.5cm.whole auricle composite tissue transplantation was used in 6 patients,the area of the graft was 0.5cm×0.5cm~0.8cm×1.2cm.results all grafts survived.the grafts were dark red or light red in color,there were no blisters on them.follow-up of more than six months showed good results.conclusion in many methods for repair of defects of alae nasi,this method is simple,the appearance of alae nasi is good after operation and there is no new scar on face.

目的 修复鼻翼缺损。方法采用全层或部分耳廓复合组织游离移植的方法修复鼻翼缺损20例,其中以鼻翼残存瘢痕组织翻转作衬里,然后进行部分耳廓复合组织游离移植14例,移植物面积最小1.0cm×1.5cm,最大1.9cm×2.5cm;利用耳廓复合组织全层嵌入游离移植修复鼻翼缺损者6例,移植物面积最小0.5cm×0.5cm,最大0.8cm×1.2cm。结果 20例移植组织全部成活。色泽呈暗红至淡红不等,无水泡。随访半年移植物色泽与鼻组织基本近似,供区隐蔽。结论在诸多鼻翼缺损修复方法中,该方法简便易行、术后鼻翼外形好,面部显著部位不增加新的瘢痕,有独到优越性。

Up till now, the main pump types used in CPB are roller pump and centrifugal pump, but these two kinds of pump both have their own disadvantages. When roller pump is used, the blood cells will be destroyed severely and when the pipeline is kinked, air will be transported into body or the conduit will be broken. The function of centrifugal pump is better than that of roller pump, but now, this kind of pumps are all designed for adults, and when the flow rate is low, the function of protecting the blood is not significant.

目前,滚轴泵和离心泵是心肺转流中的常用泵型,但是滚轴泵对血液有形成分的破坏较严重,而且有引起泵管的崩脱和大量气体泵入体内的危险性;离心泵虽然在性能上优于滚轴泵,但目前的离心泵大都是为成人设计的,而且在低流量时在血液破坏方面的优点不明显,此外它们还有一个共同的缺点就是预充量均大,因此两者都不适合在幼婴儿体外循环中应用。

Thousands of SCR-584's were built. They were used in North Africa, and all the way from Anzio, Italy up through Europe. They were heavily used in the aerial Battle of Britain.

SCR-584生产了数千套,在北非,在安齐奥,在意大利直到欧洲,并在不列颠之战担当重任。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。