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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

All of us come up with one answer, you come up with the complete opposite.

所有的人都得到同样的答案而你出来的是完全相反的

By analyse thus complexion,we use BORLAND's DELPHI , using it's offer's OO empolder tool, especially data-window, it can convenience and concision to control the database of intelligentize object, first of all, at short notice set up system apply anteeype, and then, original antetype system put up requirement repeatedly, continually amend and mend, till come into being user well-pleasing's system.

经过分析如此情况，我们使用BORLAND公司的DELPHII开发工具，利用其提供的各种面向对象的开发工具，尤其是数据窗口这一能方便而简洁操纵数据库的智能化对象，首先在短时间内建立系统应用原型，然后，对初始原型系统进行需求迭代，不断修正和改进，直到形成用户满意的可行系统。

By analyse thus complexion,we use BORLAND's Delphi , using it's offer's OO empolder tool, especially data.window, it can convenience and concision to control the database of intelligentize object, first of all, at short notice set up system apply anteeype, and then, original antetype system put up requirement repeatedly, continually amend and mend, till come into being user well.pleasing's system.

经过分析如此情况，我们使用BORLAND公司的DelphiIDelphi，利用其提供的各种面向对象的Delphi，尤其是数据窗口这一能方便而简洁操纵数据库的智能化对象，首先在短时间内建立系统应用原型，然后，对初始原型系统进行需求迭代，不断修正和改进，直到形成用户满意的可行系统。

Although the production of relatively small achromatic and apochromatic refractor telescopes of up to 15cm, and catadioptic systems of up to 18cm, all with a variety of accessories at Jena would continue until 1994 when it was realized that Zeiss could not compete in the world market; the Zeiss "APQ" refractor telescopes optics were among the finest in the world but the inability to Zeiss to adapt these products promptly to the realities of a free marketplace assured their demise.

虽然耶拿厂可以生产相对小点口径的消色差和复消色差折射望远镜（口径最大可达15cm），还有口径最大可达18cm的反射折射望远镜系统，而且产品规格类型丰富，但蔡司在1994年还是意识到，不能在此类产品的世界市场上继续竞争了；蔡司的"APQ"系列折射望远镜产品确实属于世界顶级产品之列，但蔡司不具备在自由市场经济环境下，迅速调整改进此类产品的能力，所以决定此类产品停产。

Even until advanced age about 70 years old she still comes out again to see a matter,.Chanel group is in 1983 served as a vogue director general by Karl Lagerfeld, but the up to now each quarterly new product still take Chanel spirit as in order to design a principle, Chanel, lasting Chanel.Quote from a words in Chanel:"I not apprehensibility woman why can't just for the sake of the performance manner, before go out all dress up well once, every day who know to can be to in the clout a predestined big day?"

甚至一直到70多岁的高龄她都还复出视事，香奈儿集团在1983年由Karl Lagerfeld出任时尚总监，但至今每一季新品仍以香奈儿精神为为设计理念，香奈儿，永远的香奈儿引用香奈儿一句话："我不能理解女人为何不能只是为了表现礼貌，出门前都好好打扮一下，每一天谁知道会不会是命中注定的大日子？"

Traditionally,Aristotelian logic,which dominated the logic scene for over 2 00 years(up to the beginning od the twentieth century),dealt almost exclusively with argument made up of sentences like "All A are B" and "some A are B".

按照传统的观点，统治了逻辑学界2000年之久的亚里士多德逻辑(直至20世纪初)，几乎唯一地处理由诸如句子"所有的A是B"以及"一些A是B"所组成的论证。

At the stroke of midnight, they all "flipped their lids" and the year on their foreheads lit up in conjunction with the numbers "1908" on the parapet of the Times Tower lighting up to signal the arrival of the new year.

在午夜敲响钟声的那一刻，他们都纷纷"打开帽子开关"，他们额头上的小灯和时代塔栏杆上点亮的1908字样交相呼应，迎接着到来的新的一年。

In the light of cost index and pollution degree, the characteristics of kinds of pulp are analyzed in detail, and the GP pulp which is low cost, high-yield, no-pollution is chosen as the material of tableware. Based on material element analyzed, shaping technology is designed, the to and for single plane shaping equipment which is high shaping quality, high production efficiency and is easy to automatize is chosen. All kinds of measure are taken to solve the problems which are inequality in the shaping process of GP pulp, and dosage which matches with GP pulp is chosen. Some ways of fixing shape and heat up are analyzed, the technology of saving energy of heat pressing to fix shape being composed of oil heat and only one fixed shape in the mold is applied, with which it is high production efficiency, low cost to fix shape effectively, which saves energy 30 percent. In order to make the production line meet with material of GP pulp, measure are taken to solve the problem of pulp adhered to netlike molding which can lead to carry material difficult, the structure of fully automatic equipment is renovated, the loaded beam is optimizationally designed to make equipment structure compactand improve location precision and products quality. According to technologic flow and actual characteristic, Omron PLC is chosen as the core of the control system, proximity switch as detect unit take the place of touch switch, software is designed reasonably according to time order figure, hardware is simplified, the reliability of system is advanced, the stability of system is assured to make production order. Intelligence neuron PID system is studied theoretically which is made up of both neuron with self-organizing and self-learning function and ordinary PID with the characteristic of the simple structure and high stability, which can automatically tune the parameter with the change of system parameter, remain perfect performance.

按成本指标和污染程度分析各浆料的特点，选择低成本、高得率、无污染的磨石机械浆为原料，在原料组分分析的基础上，设计成型工艺，选择成型质量好、生产率高、易实现自动化的往复式单板成型机，采取各种措施解决GP浆成型不均匀的问题及选择与机械浆相匹配的助剂；分析几种定型方式和加热方式，采用定型效果好、生产率高、成本低的油加热与模内一次性定型相结合的热压定型节能技术，节能30％；为了使生产线适应磨石机械浆原料，采取相应措施解决由于粘网造成物料转移困难，对全自动设备结构进行改进设计，着重对物料传送系统的关键部件——承载梁的进行优化设计，使设备更加紧凑合理，提高设备的定位精度和产品质量；根据生产线工艺流程和现场特点，选用OMRON CPM1A型PLC为主核心，接近开关作为检测元件，替代有触点开关，选用可编程终端代替常规面板按扭，并按照时序图科学合理地设计软件，有效地简化系统硬件构成，提高系统的可靠性，保证系统的稳定性，从而使生产更加通畅有序；从理论上探索将神经元应具有的自组织、自学习要求与PID控制结构简单、稳定性好的全自动纸浆模塑餐具生产线的研究特点结合起来，构成神经元自适应PID控制器，当参数的变化时，系统能自行参数整定，保持优良的工作性能指标，满足实际需求。

One thing's for sure, we won't give up because all of us want to get back up the order.

当然有一件事情是肯定的，我们不会放弃，因为我们所有人都想回到原来的位置。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。