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METHODS: 40 cases were classified stage IV with lumen obstruction after they failed all other treatment regimens. PDT was performed witn 630nm light delivered through cylinder diffusing tip quartz fibers passed through the biopsy channel of a flexible endoscope 48h after intravenous injection of the photosensitizer photofrin. PDT endoscopy was repeated and necrotic tissue was mechanically removed and, if necessary, that site or other new sites were treated after 48 hours. Two days after this another endoscopy was performed and the necrotic tissue was mechanically removed. Endoscopy was repeated one month after PDT and periodically thereafter as needed to re-treat symptomatic residual tumor.

40例肿瘤患者均属于IV期，所有患者都经过化疗、放疗或其他方法治疗无法解除腔道梗阻或不完全梗阻，部分完全梗阻患者结合其他治疗手段解除梗阻，光敏剂为photofrin，按2mg/kg体重静脉滴注，48小时后经内镜导入光导纤维给以630nm（DIOMED半导体激光治疗机）的激光照射，2天后经内镜清除坏死组织并对原有病灶和新发现病灶给以复照，之后根据具体情况给患者的病灶部位清除坏死组织。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Are all purchases (materials, merchandise, services, and expenses) routed through a purchase register or voucher record and not directly through cash disbursements?

所有采购业务（包括原料、机械设备、服务和费用）均按制定的采购登记或凭证记录程序进行，而非直接通过现金支付？

All can not go into details to communicate through the introduction of enterprise information, business history, part of the corporate culture only through a clear positioning, the logic of rigorous, coherent corporate propaganda in order to load pictures.

一切无法通过赘述进行沟通介绍的企业资料、企业历史、企业文化等部分，只有通过定位清晰、逻辑严谨、条理分明的企业宣传画册才能承载。

The Church does not grow through proselytising, but through "attraction", just as Christ "draws all people to himself" with the strength of his love.

就如基督是藉着爱的力量&吸引众人归向他&；同样，教会的成长不在于说教而在于&吸引&。

Modification caption to the website especially the website of tall Pr is very adverse, but the long-term rank that should make good website must mix the homepage the caption of each minutes of page has been done, accordingly, await in the begining advocate the rank of mainstream keyword Google of the station drops quickly from the 2nd page 150 the result that caption revises basically is beyond the name, and link of a large number of dead catenary of website itself, invalid friendship, rubbish friendship link, mix through Title, Meta picture Alt attribute, inside of catenary optimize, of the article update, oppose catenary and written language of the anchor that return chain through recommending site of guest of many forum, rich, tall Pr especially, today before the rank of mainstream keyword Google that All Souls'Day stands was entered 20, opposite before have promotion.

修改标题对于网站尤其是高pr的网站是非常不利的，但是要做好网站的长期排名就必须把主页和各分页的标题做好，因此，开始时候主站的主流关键词Google排名从第二页急剧下降到150名以外主要是标题修改的结果，以及网站本身的大量死链、无效友情链接、垃圾友情链接，通过title、meta和图片alt属性、内链的优化、文章的更新，尤其是通过引进大量的论坛、博客、高pr站点反链及反链锚文字，今天主站的主流关键词Google排名进入了前20名，并相对之前有提升。

If they were to build big machines, they would obviously need more space, but the idea of building a new building to house a new machine was not thinkable in the Cavendish culture. Rutherford was notoriously stingy and kept all expenditures to a minimum. The Cavendish was a historic building and could not be remodeled. So everything that Cockcroft and Walton built had to fit into existing rooms and pass through existing doors. This had the consequence that they could not use any commercial power equipment that could not be squeezed through the historic Gothic gateway of the Cavendish. They had to spend long months designing and testing their own equipment.

他们要修建一台大型设备，很明显，他们需要比现有房子更大的空间，但盖一栋新房子来装一台新机器的想法在卡文迪什的文化中不可想象：卢瑟福的小气赫赫有名，他把一切花费压到最低限度；卡文迪什是一栋历史建筑，不能改造，所以考克饶夫和华尔顿建造的一切设备不得不适应现有的房间和门的大小，这使得他们无法使用任何商业动力设备，因为那些设备无法挤进卡文迪什具有历史意义的哥特风格大门，他们不得不花很长时间来设计和调试自己的设备。

Rough determination,through study,through struggle...all in defiance of a thun

的调整，而地球却在脚下颤抖，有了我谁还需要上帝？

Rev. Morrison received three years of British education on theology, philosophy, mathematics and Chinese. In January 1807, Rev. Morrsion went aboard "the Remittance" leaving the Great Britain on a mission to learn the Chinese language, edit the Chinese dictionary and translate the Bible."The Remittance" crossed the Atlantic and reached New York in May, where he connected via the Trident that sailed through the Cape of Good Hope in Africa and Indian Ocean. His arrival required a final leg that went through Singapore and the strait Sunda between the islands of Sumatra and Java towards the China Sea. All the routings and connections of the trip was a result of the East India Company's refusal to allow Rev.

马礼逊牧师在英国接受了三数年的神学、哲学、数学及中文等训练，於一八○七年一月底，以学习中文、编纂中文字典以及翻译圣经等任务，乘「汇款号」轮船离开英国，横渡大西洋抵纽约，五月中转乘「三叉戟」号轮船经非洲好望角，横渡印度洋，穿越苏门答腊海峡，经新加坡，往东北进入中国海前往中国，这样转折耗时的旅程，皆由於东印度公司恐影响在华贸易，拒绝伦敦会传教士乘搭公司轮船来华。

He doesn't hear all the tiny details that went through my mind while I worked through my rask; he hears only what he needs to know -- information that is fermented and distilled again and again by his staff.

他不太在意我们自己在工作期间所思考的细微末节，他注重的只在於他所必须知道的资讯－是经过工作成员不断反覆思辨、萃选后的资讯。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。