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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

Pening range breakout is effective after an inside day that has a smaller daily range than the previous four days (IDnr4 in Figure 1 ) and after any day that has a daily range less than the previous six days (NR7) whether an inside day or not.

RB这一现象发生在IDnr4或者NR7之后常常是有效的。IDnr4是指振幅小于前面4天的孕线形态。NR7是指振幅小于前面6天的形态。

Keeping this in mind, a study was undertaken to determine the effect of CA on early growth of mung bean (Phaseolus aureus=Vigna radiata), morphogenetic response in hypocotyl cuttings of mung bean, and changes in the activities of some associated enzymes – proteases, polyphenol oxidases, and peroxidases – and endogenous total phenolics at root initiation (third day; 48 h; appearance of root primordia; RI), root expression (fifth day; 120 h; appearance of roots; RE), and post-expression (seventh day; 168 h; PE) stages of rhizogenesis.

保持这一点，一项研究，以确定影响钙对早期生长绿豆（ phaseolus金黄色葡萄球菌=豇豆拉迪雅塔），形态发生反应，下胚轴插条绿豆，和变化，在活动的一些相关的酶-蛋白酶，多酚氧化酶，过氧化物酶-和内源性总酚的从根本上启动（第三天； 4 8小时；外观根原基；里），根的表达（第五天； 1 20改为H级；外观根；转口），和后表达（第七天； 168改为H级； PE ）的阶段rhizogenesis 。

In summer-autumn,2002,we developed treat-patient-free activity:on " Army Day "、" Teacher Day " and " National Day ",the patient may be free of registration fee,derate 10% of medicines'fee.In Aug.2002,we established health school for citizens:every month a famous expert makes lectures on popular science,for free of surveying blood sugar and blood pressure on the spot,at the same time,students may gain free membership certificate of healthy school and enjoy more preferential treatment.

以人性化的服务，打造&人文关爱工程&。2002年夏秋季开展人文关爱的系列义诊活动，具体为&建军节&、&教师节&和&国庆节&开展为患者服务，患者就诊免交挂号费、药品费减免10%。2002年8月在省内首创为市民免费讲座的健康学校，每月由一名知名专家就医学知识进行科普讲座，现场免费对听众测血糖、量血压，同时，听课者免费获得健康学校会员证，会员来院就诊享有多项优惠待遇。

Results In the group of normal rats with partial hepatectomy, immunohistochemistry staining indicated that the expression of HGF and C-met increased to get the peak point after partialhepatectomy 12 hours and 3 days respectively, and HGF maintained at the high level to the 7th day, then decreased gradually, finaly approched to the level of pro-operation at 14th day, but C-met fell sharply,and declined to the the level of pro-operation at the 14th day.

结果 免疫组织化学显示，正常肝部分切除组，HGF表达于术后12 h达高峰，并维持高峰平台，7 d后逐渐降低，于14 d接近术前水平；C-met术后迅速上升，3 d达到高峰，而后逐步下降，14 d降回术前水平。

With initial immunization at day 70 and a booster at day 98, the growth performance of the yellow-feathered broiler chickens exhibited a stage-wise pattern up to slaughter at the end of the 49-day postinoculation trial period.

在经过首免，28天后的二免，到119天龄屠宰间，黄羽肉鸡的生长性能表现出阶段性的特点。

I cite a case, price of this word contest is as high as tumor 16 yuan are clicked every time, one day clicks the word of 1000 is then 16 thousand yuan everyday, lead the 1% words that will calculate by changeover (conservative estimation is inside this limits), so a day of 1000IP the word has 10 people is duped, 16 thousand yuan of affirmation of a day want these 10 people to come namely burden, so be equivalent to you treat disease most most the injustice money that the most conservative affirmation should give is the least 1600 yuan (affirmative of course and more than this, the person that just says to see a doctor here also should do a good turn cost of price giving contest, sweat).

我举个例子，肿瘤这个词竞价高达16元每次点击，一天点击1000的话那就是1.6万元每天，按转换率1%来算的话，那么一天1000IP个话有10个人是上当的，一天的1.6万元肯定就是要这10个人来负担，那么相当于你去看一次病最最最保守肯定要出的冤枉钱最少是1600元(当然肯定不止这个，我这里只是说看病的人也要帮忙出竞价费，汗)。

Soaks the coffee to let you warm the hand to want to keep off in your Chest's wind, you actually want to take to the streets to blow the cold wind to be much more sober, you said that you did not fear bids good-bye only then a regret to be sad, the valentine day must come to remain oneself one, actually liked the right person valentine day crossing every day.

泡咖啡让你暖手想挡挡你心口里的风，你却想上街走走吹吹冷风会清醒得多，你说你不怕分手只有一点遗憾难过，情人节就要来了剩自己一个，其实爱对了人情人节每天都过。

The idea has spread widely to other lands where it is variously celebrated as the 'Festival of Trees','Greening Week' of Japan,'The New Year's Days of Trees' in Israel,'The Tree-loving Week' of Korea,'The Reforestation Week' of Yugoslavia,'The Students' Afforestation Day' of Iceland and 'The National Festival of Tree Planting' in India. Arbor Day in its various forms is now recognised in more than fifty countries.问题补充: On Arbor Day, particular attention is drawn to the part trees play in our lives.

想法广泛传播了对它不同地庆祝作为树的'节日的其它土地，'绿化的星期'日本，'新年树的在以色列，'喜爱树的星期'韩国，'重新造林星期'南斯拉夫，'学生的植树造林天'冰岛和'全国节日在树荫处天，特护被引起对零件树演奏在我们的生活中。

My target: 1, every day being joyful , enriching every day, carry out self keep healthy , finish grasping from the target every day.

我的目标：1，每天都是开心，每天都充实，每天都从目标进行，把握好自己的身体健康。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"