查询词典 alizarin blue

The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen.

结果表明，水淹或乙醚麻醉处死、酒精固定、3%重铬酸钾脱脂、氢氧化钾透明、茜素红硬骨染色、阿利新蓝软骨染色、甘油-氨水漂白、甘油逐级透明的制作方法，获得的透明标本效果最佳。

In the biochemical analysis aspect, it is first time to use the simple fluorescence spectrophotometer to determine the protein in human serum by red alizarin as the probe of the RLS. The experiment shows that the system HSA-red alizarin has the maximum intensity of the RLS in Britton-Robison buffer of pH4.10 at maximum wavelength of about 360nm. The method has better linear range within 0.040-10.0ug/mL, determination limit is 0.040ug/mL. Moreover, We have selected three other dyes of chromotrope 2R, acid chrome dark blue and pyrocatechin violet as the probes of the RLS in determining protein in human serum and achieving satisfactory results.

在生化分析方面，作者首次使用简易960型荧光分光光度计，利用茜素红作为共振光散射光谱探针对人血清中的蛋白质含量进行了测定，测定结果显示在pH4.10的Britton-Robinson缓冲溶液，波长360nm处出现最大的共振光散射强度，其线性范围为0.040～10.0μg／mL，检出限为0.040μg／mL；另外，作者还筛选了其他三种可用作蛋白质测定的共振光散射光谱探针的有机染料：变色酸2R、酸性铬深蓝、邻苯二酚紫，并深入研究了测定的适宜条件和干扰情况，将这三种有机染料用于人血清中蛋白质含量的测定，均取得了满意结果。

C. I. Reactive blue KN-R, Alizarin brilliant green G and Hostlam blue R could not be degraded by Zoogloea HP3. Furthermore, ABAS degradation was regressed under the exisitence of the above dyes. 1-Aminoanthraquinone-2sulfonic sodium, 1, 4-dihydroxylanthraquinone-2-sulfonic sodium, 1, 4, 5, 8tetrahydroxylanthraquinone, anthraquinone, aniline, phenol, catechol and ophthalic acid could be degraded by Zoogloea HP3. Aniline was the most degradable substrate among ABAS, aniline, phenol, catechol and o-phthalic acid. However, benzenesulfonic sodium and p-amino benzenesulfonic sodium were not degraded by it.

动胶菌HP3不能降解活性艳蓝KN-R、弱酸性绿GS及毛用活性蓝HW-R等蒽醌型染料，且染料的存在对菌体降解溴胺酸有不同程度的抑制作用；动胶菌HP3可以降解1-氨基蒽醌-2-磺酸钠、1，4-二羟基蒽醌-2-磺酸钠、1，4，5，8-四羟基蒽醌、蒽醌及苯酚、邻苯二酚、苯胺、邻苯二甲酸等苯系化合物，而不降解苯磺酸钠、对氨基苯磺酸钠；在溴胺酸、苯酚、邻苯二酚、苯胺、邻苯二甲酸中，苯胺是动胶菌HP3的天然底物。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

Objective To compare compound staining of Alizarin red S and Alcian blue showing ossified skeletal structures and cartilage in fetal, neonatal and adult rat.

目的探讨显示不同时期大鼠骨骼和软骨的复合染色方法。

A novel determination method of trace chromium in waste water by dual-wavelength spectrophotometry was established based on the fact that chromium can react with alizarin blue S to form red complex in H2SO4 medium.

在H2SO4介质中，铬与茜素蓝S形成红色产物，据此建立了双波长分光光度法测定废水中痕量铬的新方法。

Methods: Inbred WKAH/Hkm rats were used for this study. Pregnant females were treated with busulfan at embryonic day 11. The embryos were removed at E12 to E21 and stained with alcian blue and alizarin red S. The abnormal changes in the treated embryos' hind limbs were observed with a microscope.

近亲交配的WKAH/Hkm大鼠被用于这项研究，妊娠的雌性大鼠在妊娠11天用白消安处理，妊娠12天到21天的胚胎被移开并用阿辛蓝和水溶性茜素红s处理染色，处理后的胚胎后肢的变化可以在显微镜下观察到。

Reagents and instruments: valproic acid sodium, retinoic acid, Alcian blue GX, Alizarin red S, fluorogold were provided by Beijing Phetx Co.

体重240一3009健康成熟未孕的Wistar大鼠，分别在确定怀孕的第9天9：oo、16：oo，按实验设计据体重计算剂量后后肢皮下注射丙戊酸钠溶液，浓度20%。

Trypan-blue and alizarin red vital staining was capable of displaying the cellula appearance and distinguishing the normal and abnormal cells.

光镜下可清楚显示晶状体上皮细胞的形态并能区分正常与死亡的细胞。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。