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The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20%胎牛血清+2mmol/L谷氨酸胺)进行培养;HGF诱导组以常规培养液+促肝细胞生长因子(HGF,25ng/ml)和地塞米松10~(-7M进行培养;HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF,25ng/ml)和地塞米松10~(-7/M+10%的左归丸含药血清进行培养;条件培养液加对照血清组以常规培养液+50%的条件培养液+10%正常大鼠血清进行培养;条件培养液加八珍汤组以常规培养液+50%的条件培养液+10%八珍汤含药血清进行培养;条件培养液加左归丸组以常规培养液+50%的条件培养液+10%左归丸含药血清进行培养。

Atlas air compressor fittings, maintenance kits, repair kits, coolers, atlas oil, oil and gas separator, pressure switches, solenoid valves, valve parts repair kits, hose assemblies, bearings, sleeve, belts, Sweden Alfa Laval oil coolers, Fusheng air compressor fittings, air compressor fittings°,°Sullair air compressor oil SULLUBE32, Sullair air compressor cooler, Sullair Air machine-specific oil, Sullair air compressor fittings Fusheng advanced cooling fluid,°Fusheng air compressor oil cooler (4000 #)(2000 #), Hitachi oil cooler, oil Berg, Inge Solan original cooler air compressor, air compressor fittings, the United States Ingersoll-Rand Super coolant, Ingersoll-Rand original fittings, Shenzhen Sullair, original fittings, Fidelity air compressor, air compressor Liuzhou Fidelity original fittings, original coolers, oil (LT3046) Germany Julius Caesar, Caesar oil, Germany Burg Burg compressor oil Taiwan Fusheng, Fusheng air compressor oil, coolant Advanced JAPAN Hitachi Air machine oil, Japan KOBELCO, KOBELCO compressor oil, Japan Mitsui various air compressor oil air compressor oil separator, the core oil filter, air filter Atlas, Ingersoll-Rand, Shou edge,°, Fidelity and other brand-screw air compressor oil, genuine parts, nose repair, air compressor maintenance works

阿特拉斯空压机配件,保养包,维修包,冷却器,阿特拉斯专用油,油气分离器,压力开关,电磁阀,阀件维修包,软管组件,轴承,轴套,皮带,油封瑞典阿法拉伐冷却器,复盛空压机配件,康普艾空压机配件,康普艾空压机专用油寿力SULLUBE32,寿力空压机冷却器,寿力空压机专用油,寿力空压机配件复盛高级冷却液,复盛空压机冷却器康普艾专用油(4000#)(2000#),冷却器日立专用油,伯格专用油,英格索兰空压机原装冷却器,空压机配件,美国英格索兰超级冷却剂,英格索兰原装配件、深圳寿力,原装配件,富达空压机,柳州富达空压机原装配件,原装冷却器,专用油(LT3046)德国凯撒,凯撒专用油,德国伯格,伯格空压机专用油台湾复盛,复盛空压机专用油,高级冷却液日本日立空压机专用油,日本神钢,神钢空压机专用油,日本三井空压机专用油各种空压机油分离器,芯油过滤器,空气过滤器阿特拉斯,英格索兰,寿力,康普艾,富达等各种品牌螺杆空气压缩机油、正品配件、机头大修、空压机维护保养工程

Rainbow Xuzhou country air adhering to the "customer is always first" service concept, sharing the U.S. commercial air conditioners, home air conditioning Gree, Haier air conditioning, Daikin air-conditioning, Mitsubishi air conditioning, newly air-conditioning, air conditioning CHIGO, Chunlan air-conditioning, Panasonic Air Conditioning Product enormous platform to rely on the existing scale and highly uniform service channels, professional service team, with sincere action to meet customer demand. Heart to serve the public well-being of users www.gh365.net Living Rainbow Xuzhou States Air conditioning air-conditioning Co., Ltd. Xuzhou Golden MAG Air Conditioning Co., Ltd. Xuzhou Rainbow Enterprises State Services Limited

苏州国虹空调秉承"顾客永远是第一"的服务理念,共享美的商用空调,格力家用空调,海尔空调,大金空调,三菱空调,新科空调,志高空调,春兰空调,松下空调巨大的产品平台,依靠现有规模且高度统一的服务渠道、专业化的服务队伍,用真诚的行动满足客户的需求。

The main work and the originality points of this dissertation could be concluded as the following parts:(1) To analyze systematically the mathematical model of LES of air flow in great space, under the case of isothermal and non-isothermal, as well as the combined action of buoyancy and strain. Some basic problems are studied about the LES application into airflow simulation of great space, such as boundary conditions, spatial difference scheme and time advancing scheme, time step etc., which creates the basis for the LES application to indoor airflow simulation.(2) Both the instantaneous and time averaged flow field of great space with multiple jets under isothermal and non-isothermal cases are explored with large eddy simulation method, and also discussed the dynamic characteristics and the law of fluidflow in the great space.(3) Based on the results of LES of the great space and the Fanger thermal comfort indexes, it is brought forward the concept of the series of dynamic thermal comfort evaluating indexes, which could be divided into thermal comfort index with time averaged properties PD, PPD, PMV, thermal comfort index with instantaneous properties IPD, IPPD, IPMV, and time averaged thermal comfort indexes TAPD, TAPPD, TAPMV, and transient situations time averaged thermal comfort indexes TTAPD, TTAPPD, TTAPMV. The differences among them and the calculation methods are discussed, and the four kinds of indexes are calculated with the thermal comfort index PD as an example.(4) Based on the LES results it is discussed the hot air stratification phenomena in air-conditioned buildings in the case with air supply and return registers on the ceiling and the case on the sidewall. The fundamentals of the hot air stratification are studied and the relations of estimating hot air stratification are brought forward.(5) With the advanced apparatus such as hot wire film anemometer IFA300 and laser particle field anemoscope, corresponding model test and site measurements have been done, which are compared with the simulation results and LES is proved a very promising method in air flow simulation indoor.

本文主要工作既创新点体现在以下几个方面:(1)系统分析了等温、非等温和考虑剪切力与浮升力综合作用的高大空间大涡模拟数学模型,并研究了高大空间大涡模拟在室内气流仿真应用中的一些基本问题,如边界条件、空间离散格式和时间推进格式、时间步长选择等问题,为大涡模拟在室内气流计算中的广泛应用打下了基础;(2)首次用大涡模拟方法研究了高雷诺数下高大空间多射流在等温、非等温情况下的瞬时流场和时均流场分布,并探讨了多射流流场的动态特性和流动规律;(3)基于大涡模拟的动态仿真结果和Fanger的热舒适指标,首次系统地提出了动态热舒适评价指标体系的概念:即基于时间平均参数的热舒适指标PD、PPD、PMV;基于瞬时参数的瞬时热指标IPD、IPPD、IPMV;基于时间平均热舒适指标TAPD、TAPPD、TAPMV;以及沿行动迹线的时间平均热舒适指标TTAPD、TTAPPD、TTAPMV,并分析了四类热舒适指标的差异性和计算方法,还以PD值为例对四类指标分别进行了计算;(4)基于数十种工况下空调房间大涡模拟的结果,研究了空调建筑上送上回和侧送侧回两种情况下热分层现象,并探讨了热分层的基本规律,首次提出了避免热分层现象的判断公式;(5)在暖通空调领域,首次使用IFA300热线风速仪、激光粒子速度场仪等先进设备,完成了与大涡模拟相对应的模型试验,并把实测结果与仿真结果进行了对比,说明LES在室内气流仿真方面是一种很有前景的方法。

Shenling has drafted the following national standards independently: air handling units for operation room, entire fresh air type dehumidifier units and low temperature packaged air conditioners.shenling has taken part in drafting the national standards: dehumidifiers, energy efficiency limit value of the chillers and its energy efficiency ratio grade, energy efficiency limit value of the packaged air conditioner and its energy efficiency ratio grade, rooftop air-conditioners, vapor compression cycle chillers, for industry and commerce use and similar purpose chillers, packaged air conditioners, changeable air amount terminal units, aeration equipment for air energy reclaiming, core documents of quality standards for constructing project products, positive displacement compressing condensers, heat pump chillers,modular air handling units,screw type refrigerant compressors, purchase specification on heating ventilating and air conditioning equipment for chemical plant.

参与起草的16项国家或行业标准是:gb/t 19411-2003《除湿机》、gb 19577-2004《冷水机组能效限定值及能源效率等级》、gb 19576-2004《单元式空气调节机能效限定值及能源效率等级》、jb/t 10538-2005《防爆除湿机及空调机》、gb/t 20738-2006《屋顶式空气调节机组》、gb/t 18430.1-2007《蒸气压缩循环冷水机组第1部分:工业或商业用及类似用途的冷水机组》、gb/t 17758《单元式空气调节机》、jb/t《空调变风量末端装置》、gb/t 21087-2007《空气-空气能量回收通风装置》、《建设工程产品质量标准核心条文》、gb/t 21363-2008《容积式制冷压缩冷凝机组》、gb/t 21362-2008《商用或工业用及类似用途的热泵热水机》、gb/t 14294《组合式空调机组》、gb/t 14296 《空气冷却器与空气加热器》、gb/t 19410《螺杆式制冷剂压缩机》、hg/t 20697-2007《化工暖通空调设备采购规定》。

All weighing tests should be made in the standard atmosphere for testing textile.after the specimens have been conditioned in the same atmosphere.it may be impractical to condition the specimens in option A or nonconditioned testing may be agreed upon by the purchaser and supplier.when the full rolls or bolts of fabric can not be properly conditioned in a resonable time with available facilities,perform the tests without conditioning and report the actual conditions prevailing at the time of the test.such results may not correspond with the results obtained after testing adequately conditioned specimens in the standard atmosphere for testing textiles.

D1776实际操作的定向试块。纺织品的所有的权衡测试都应该在标准大气压下进行。当试块被置入同等气压的条件之后,或许将试块限制于A选项是不切实际的,而且有可能条件测试并不一定会被供应商和客户认同,当充满凹凸不平和毛刺的织物不能被适当的限制与合理的时间和现有的设施的时候,在没有限制的情况先执行试验并准确的报告在测试时的有力条件。这样得到的对纺织品测试的结果或许会与在真空中得到充分限制测试的结果不相一致。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片,采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上,不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层,培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Now the thermal comfort indexes commonly used are ASHRAE Standard 55-1992 and ISO7730, which are both based on young people in Europe and unsteady indoor environment. In fact, it is not quite fit for Chinese people in semitropics and in passive non-air-conditioned buildings, as China has different climate and custom from European and the indoor environment in passive non-air-conditoned building is unsteady, which is different from air-conditioned building, either.

现行通用的ASHRAE55-1992标准和ISO7730标准都是针对欧美国家青年人提出的,并多以稳定的空调室内环境作为分析基础,因此对于我国多样气候环境下的亚洲人群而言,该类标准在适用性上将会存在一些偏差,同时也不能对被动式太阳能建筑室内非稳态环境下的人体舒适性做出十分准确的预测。

Assistance to the Air Force to carry out the expansion. After the war, into the development phase of the restoration, completed basic combat training and logistical support systems, combat aircraft to achieve the beginning of jet. 60 era for the expansion and gradually equipped with F-86D, F-5A and F-4F fighter. 80s equipped with F-5E/F, F-16 fighter aircraft, air defense automated alert system officially running. In July 1991, the Army air defense artillery and troops under the command of the Air Force formed to form a radar, aircraft, surface to air missile (anti-aircraft) integrated air defense system. 90, to receive from the U.S. military operational command, weapons and equipment have been developed, the first home-made aircraft KF-16 aircraft factory, F-16 fighters equipped with AIM-120 advanced air-to-air missile in the distance, procurement from France,"Northwest Wind "-air missile. 2002 selection of a new generation of F-15K fighter jets.

战后,进人恢复发展阶段,基本建成作战、教育训练和后勤支援系统,作战飞机开始实现喷气化。60年代进行扩编,逐步装备F—86D、F—5A和F—4F型战斗机。80年代装备F—5E/F、F—16型战斗机,自动化防空警戒系统正式运行。1991年7月,陆军防空炮兵司令部及所属部队划归空军建制,形成雷达、飞机、地空导弹一体化防空体系。90年代,从美军接收作战指挥权,武器装备也得到较大发展,首架自制KF—16型飞机出厂,F—16型战斗机装备AIM—120型先进中距空空导弹,从法国采购"西北风"式地空导弹。2002年选用F—15K新一代战斗机。

The main reason is that many high-grade office space is equipped with all-air central air conditioning, is to first deal with air, reducing temperature and humidity, the air sent through the pipeline to the room through the return air, pipeline to return to the central processing system, central processing system for cooling air return after降湿part of the new wind coupled with the second round after the blast, and so the cycle, while the central air-conditioning system for energy conservation, often adding a little fresh air, return air devices in the recovery rate of 80%, resulting in cross-infection the opportunity to increase significantly.

主要的原因是,许多高品位的办公空间配备了全空气中央空调,是先处理空气,降低温度和湿度,空气通过管道传送到房间通过回风管道返回中央处理系统,中央处理系统,冷却空气降湿后返回的一部分,新风,加上第二轮爆炸后,所以周期,而中央空调系统节能,往往增加了小新鲜空气,回风装置,回收率为80 %,导致交叉感染的机会大大增加。

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目的 观察选择性脊神经后根切除术椎板切除后硬膜外几种防瘢痕粘连物质的作用并探讨其机制。

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