查询词典 agar

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体，并通过农杆菌将上述基因转入烟草和拟南芥中，得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验，结果表明，双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生，再生芽数量平均可达13~16个/cm双鳞片，比传统的鳞茎盘之间分化提高30~50%；鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织，但以带花药的花丝诱导出的愈伤组织最好。

The stability of nutrient solution was best with 0.05% agar to be added.

结论得到了优化后的澄清工艺，提高了营养液的稳定性。

This company now has the annual production ability of 200 t of high-quality agar and 2 t of agarose.

企业目前已形成年产200吨优质琼脂和2吨琼脂糖的生产规模。

Objective To find a new semi-solid agar for bacterial motility test which is easy to judge.

目的 寻求一种对细菌动力试验结果更容易判定的半固体培养基。

The minimum inhibitory concentration was determined by serial dilution in the same type of agar, with concentration ranging from 5, 10, 25, 50, 75 and 100 mg/ml.

最低抑菌浓度测定在同一类型的琼脂稀释系列不等，5，10，25，50，75和100毫克/毫升的浓度。

Additionally, the growth of the pathogen was obviously affected by cultural media. The morphological characters such as colony color and diameter, and production of fruit body of the fungus growing on PDA, 10% V8, apple leaves dextrose agar and apple leaves extraction dextrose agar media were different. The fungus formed colonies of about 8mm in diameter and did not produce fruit bodies and aerial hyphae in 1 month incubation on PDA. However it formed the similar size colonies as on PDA and produced fruit bodies and aerial hyphae on 10% V8 and LDA media in the same incubation period. Very small colonies (2mm in diameter) and fruit bodies were found on LEDA media in the same incubation condition. These results indicated that successful isolation of M. coronaria from apple leaves depended on suitable isolation method and cultural media as well as fresh samples.

不同培养基上菌落形态、大小和产孢情况差异也很大，培养1个月（25℃）后PDA上菌落黑褐色隆起，表面蚯蚓粪状，无气生菌丝，无子实体和基内菌丝；10% V8培养基上菌落中央隆起，黑褐色，表面生少量气生菌丝，边缘放射状，基内菌丝深褐色，有子实体；苹果叶片葡萄糖琼脂培养基上菌落平坦，黄褐色，表面生茂密的金黄色气生菌丝，基内菌丝深褐色，有子实体；苹果叶片煎汁葡萄糖琼脂培养基上菌落有明显的不规则隆起，黄褐色至黑褐色，表面生少许气生菌丝，菌落生长缓慢，无基内菌丝，分生孢子盘菌落表面生，菌落直径仅2mm左右，而在其他培养基上的菌落直径可达6－8mm，说明培养基质、分离方法均对苹果盘二孢的分离培养和生长发育有明显的影响。

The strain can normal growth using agar as the sole carbon source medium and after the growth of 4-5 days, agar massive was hydrolysised with the appearance of a great amount of holes.

该菌株在琼脂作为唯一碳源的培养基中能够正常生长，并在4-5天的生长后，琼脂上产生大量的水解坑，在培养15天左右整个琼脂都被液化。

Either instrument can be used to obtain the inoculum from an agar slant culture by carefully touching the surface of the solid medium in an area exhibiting growth so as not to gouge into the agar.

任何一种器具都可以用来在洋菜斜面培养上获得接种原，小心地碰触有生长的固体培养基的表面，避免刺破洋菜培养基。

Optimization technology was acetidin: N-hexane (3:7) as low-pole solvent system, methanol: dichloromethane (1:9) as polar solvent system, 20% glycerol filter paper as moisturizer, 14 h age Bacillus subtilis as indicating strain seed on thin layer plate cultivated for 14~18 h, 1% TTC staining time 1~2 h, bacterial mix method suitable to 0.2%~0.6% agar semisolid bouillon culture-medium, bacterial spread method suitable to 1% agar semisolid bouillon culture-medium.

结果：该方法展开系统筛选简单，抑菌斑位置准确、斑点清晰，其优化条件为：以乙酸乙酯：正己烷(3：7)为弱极性展开系统，甲醇：二氯甲烷(1：9)为强极性展开系统，用20%甘油滤纸对湿室保湿，以14h菌龄的枯草芽孢杆菌为指示菌种子液，自显影薄层板36℃培养14~18h，1%TTC显色时间为1~2h，含0.2%~0.6%琼脂的半固体肉汤培养基应使用混菌法生物自显影，含1%琼脂半固体肉汤培养基应使用涂布法生物自显影。

The isolates on PDA,rice polish agar,prune agarmedia produced little or no sporulation(prune agar being a little better)evenunder UV-light irradiation.

而所有菌株在马铃薯、米粉和杏汁琼脂培养基上，即使经紫外光连续照射，也不能产生孢子或产孢量少；在各种培养基上的室内自然光下都不能产生孢子或产孢量少。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷