查询词典 agar

The wheat flour was suspended in agar sol containing dibutyl phthalate and made into suspension.

将面粉悬浮于含有邻苯二甲酸二丁酯的琼脂胶体中而制成悬浮液。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

The optimum conditions were as follows: 1 Fe3+/Fe2+ was 1.70-1.75;2 the precipitant,25% ammonia water was used 20%-30%,under 35℃,pH10-11 and with a stirrer speed of 3000 r/min for 1 h;3 in the first package process with gelatin and agar,the optimum amounts was 0.050-0.060g/150mL at the temperature of 55-60℃ and pH of 9-11;In the f...

最佳工艺条件为：(1)Fe3+/Fe2+之比在1.70~1.75；(2)沉淀剂25%NH3.H2O过量20%~30%，温度35℃，pH值11，反应时间为1h；(3)琼脂的最佳用量为0.050~0.060g/150mL；包覆的最佳温度为50~60℃，pH值为9~11；聚乙二醇作为第二次包裹剂时，最佳温度为55℃，反应时间是1h，pH值为4~5；并用透射电镜、分光光度计、古埃磁天平等进行了初步表征。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

In Hubei. After screening, two strains of fungal parasites were gottenwhich had stronger. ability to parasite Meloidogyne eggs. Their parasitic ratio tothe root knot eggs was 78.04% and 70.02% in concave slides and water agar plates,72.37% and 65.65% in pots. The effective hatchings in experimental containers wereonly 5.02% and 8.32%, and there were 100% in controls.

危害的根和根围土壤64份样品，采用3种方法分离，经过筛选测定，获寄生能力较强的真菌2株，室内凹玻片和琼脂平板法测定结果表明，对卵的校正寄生率分别为78.04%和70.02%；室外盆栽测定的寄生率分别为72.37%和65.65%，供试卵有效孵化率为5.02%和8.32%，对照卵为100%。4%土重的棉籽壳真菌制剂能有效控制根结线虫第一代卵量，有效用量的10倍菌剂盆栽测定对植物均没有不良影响。

METHODS The pollu-tive status of Acanthamoeba of drinking water and cornea contact lens preservation box and preserved liquid of college students in Yanji were studied.103drinking water samples and93cornea contact lens preserved liquid and the cleaning liquid were inoculated on the surface of non-nutrient agar plates that contained heat-killed E.coli.

方法］ 对延吉市居民饮用水棘阿米巴污染情况及大学生角膜接触镜保存盒和保存液的棘阿米巴污染情况进行调查。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法，测定西帕依固龈液对牙龈卟啉单胞菌（Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度；对10例志愿者采用自身前后对照试验，观察四种干预措施（无干预、清水、西帕依固龈液、口泰）对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响，评价西帕依固龈液防治口臭的效果。

By culturing on malt extract agar medium with temper

结论］红腐乳中分离的纯培养为红曲属真菌。

By culturing on malt extract agar medium with temperature of 25 degrees Celsius,strains w...

结果从红腐乳中分离的两个菌株经鉴定M-1属丛毛红曲菌，M-3属红曲红曲菌。红腐乳中分离的纯培养为红曲属真菌。

The hyphae developed after 7 days on Malt extract agar and the teliospore-like structure was observed .

在麦芽汁液体培养基上培养三天，菌体柱状，一端出芽，细胞大小为（3-4.5）х(7.5-15)μm ，放置一个月后没有菌膜形成但有沉淀。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。