查询词典 agar

By the test of clonal growth in soft agar medium , we compared the ability of cloning and proliferating among these three kinds of cells. The cell cycle was analyzed by FCM and morphosis of cells was observed under transmission eletron microscope.

通过细胞生长曲线，比较卵巢癌细胞系HO－8910与8910－p93、8910一pCDNA3.1（转染空载体ncDNA3.l）的增殖情况；通过软琼脂克隆形成试验比较这3种细胞的克隆形成能力和增殖能力；利用流式细胞仪检测这3种细胞的细胞周期的改变情况：利用透射电镜观察转染p93基因的细胞的超微结构变化；利用裸鼠移植瘤实验比较HO七与8910个93在动物体内生长状况。

By accident, there was a mouldy fungus growing on one of his agar plates.

他偶然地发现，一个培养皿里面长出了霉菌。

The results showed that the soil microbial densities in mangroves and mudflat were significantly influenced by the salinity of dilution water and agar media.

统计分析显示细菌的海水稀释效果优于淡水，而放线菌与真菌则刚好相反(P.05，一个例外)。

It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.

本实验以菊花的茎尖为外植体，研究了不同灭菌方法，光照强度，全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响，并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响，结果表明：在初代培养物建立的过程中采用0.1％升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果；在全光照条件下外植体的增殖倍数最高；在试管苗增殖培养的过程中，以MS为基本培养基，并在培养基中添加0.2～5mg·L~(-1)6-BA和0.05～2mg·L~(-1)NAA，其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖，其分化率为100％，增殖倍数为12。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Specific siRNA was designed and synthesized, and transfected Hela with liposome siNEO FX, then analyse the role of siRNA on proliferation vitality outside the Hela cells, cells periodic distribution by MTT method, double-layer soft agar clone-forming assay, and flowing cell technology.

设计并合成siRNA，脂质体siNEO FX转染Hela细胞，分别用MTT法，双层软琼脂克隆形成试验和流式细胞术分析了siRNA对Hela细胞体外生长增殖活力、细胞周期分布的作用。siRNA作用后的Hela细胞增殖速度减慢，软琼脂克隆形成率降低，G0/G1，期细胞比率增加。

Methods: The author chose randomly sixty healthy children as the control group and one hundred and sixty children with asthma in acute period before ICS therapy as the experimental group. The experimental group were treated with ICS therapy and then eighty-nine children of this group were followed up for 3 months ,sixty-eight for 6 months and sixty for 12 months. The ICS in the study was budesonide with the trade name as Pulmicort. We adopted swab from the pharynges respectively, inoculated it in agar plate, isolated the bacteria, and evaluated the distribution and variation of the microbial population in pharyngeal portion.

方法随机选择健康儿童60例和支气管哮喘急性发作期患儿160例采取其口咽拭子，并于哮喘患儿在吸入型糖皮质激素(布地奈德，商品名普米克)治疗后分别随访3个月(89例)、6个月(68例)、12个月(60例)分别采取其口咽拭子，接种于琼脂平板，分离细菌，按程序鉴定，观察其咽部菌群的分布及其演变。

And AEV agar gel precipitin antigen and an inactivated oil-emulsion vaccine against AE were produced. The purpose of this study, on the base of former research, was to reveal and better understand some molecular pathogenesis of AE, to set up a working model which could be generally used by anther researcher in the field of animal molecular pathology.

本项实验研究，旨在过去工作的基础上，利用分子生物学、分子免疫学的技术手段，探讨和揭示AE发生发展过程中的某些分子发病学机理的问题，并试图建立一种具有普遍指导意义的动物分子病理学研究的工作模型。

Indirect enzyme-linked immunosorbent assay for detecting antibodies against avian influenza virus was developed by using expressed full length nucleoprotein of H9N2 AIV in E.coli.263 chicken serum samples(including 243 clinical serum samples and 20 positive serum samples from H9N2 AIV vaccinated chicks) were detected by NP-ELISA,agar gel precipitin test,and hemagglutination inhibition.

以大肠杆菌系统表达的H9N2亚型禽流感病毒核蛋白为抗原，建立了禽流感间接酶联免疫吸附试验抗体检测技术。

The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明，该分离病毒无血凝性，在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线；人工感染28日龄雏鸡出现与临床一致的病变，并回收到病毒；应用针对IBDVVP3基因的特异性引物进行RT-PCR，能扩增到长度为1041bp的特异性目的片段；序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

Now, however, all children continue in "comprehensive" schools, and the eleven-plus determines which courses of study the child will follow.

然而现在，所有的孩子都要在综合学校继续学习，所以这次考试只是决定他们将要学习哪些课程。

Cultivatable land , and led to a general recognition that development must not be carried at the cost of agriculture .

城区的迅速扩大在很多情况下侵占了宝贵的可耕地，使人们普遍认识到发展不能以牺牲农业为代价。