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Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

To create bump and specular maps I simply desaturated all the color map layers and changed their value and contrast accordingly, only minor local painting was required afterwards.

为了创建凹凸贴图和高光贴图，我简单降低了所有的色彩层的饱和度，根据需要改变它们的数值和对比度，然后在需要的地方做了小小的绘制。

Amid many trials and discouragements he completed his term of seven years' servitude, and having in that period come by practice, at first surreptitious, though afterwards allowed by his master "in lawful hours," to know his own powers, he removed to London.

中，许多审判和discouragements他完成他的任期7年劳役，并考虑在该段期间来的实践，在第一偷偷摸摸，虽然事后允许他的主人&在合法时，&知道自己的权力，他搬到伦敦。

The College gathered a numbers of famous educationalists; Afterwards, many of its graduates were up and doing various fields.

学校集中了一大批当时的知名教育家，学生中的很多人后来活跃在各个领域。

Afterwards, he emailed me his new works of this year.

之后，又将他今年的新作从邮箱发了一些给我。

Afterwards the experimenters checked their story comprehension and memory for the list of words.

之后，实验者检查了他们对故事的理解以及对那些单词的记忆。

If any assizes cannot be taken on the day of the county court, asmany knights and freeholders shall afterwards remain behind, of thosewho have attended the court, as will suffice for the administration ofjustice, having regard to the volume of business to be done.

如果巡回审判不能按期在郡法庭举行，足够的骑士和自由佃户应当留下来，等待那些要出席法庭的人，并且服从法官的安排，以尊重案件的解决。

Dispatch of new wall bulletin (Chen Xiaomin of reporter Yu Yalian's trainee) first afterwards Guangdong P2P serve business to be suspected of encroaching " 7 swords " after copyright case loses a lawsuit, POCO website because encroach " build up date " authority of transmission of website sth resembling a net is sentenced to compensate for.

新快报讯(记者余亚莲实习生陈肖敏)继广东首例P2P服务商涉嫌侵犯《七剑》著作权案败诉后，POCO网站又因侵犯《集结号》的信息网络传播权被判赔偿。

But it only can use one time, after once puts on cannot again take off, because extinguished actually its witch collects the strength under its influence already to surmount its own limit, too crossed the exuberant flame finally to be able to burn oneself, when it is loosened once more, the technique in instantaneous will obtain certainly the big strength, but in after, also will lose his/her body for extinguished actually all abilities Constrains the word spirit: The light depends on the technique name to be able the reflective launch technique Pulls the star box: God of death aristocrat's symbol * Breaks the Asian space newly, in order to does not let the space which the present world and the corpse soul each other contact produces impractically in the traversing break the accident separately outside the samsara soul, paces back and forth is breaking, passes through year after year gathers in together, afterwards the soul can separate the memory and the energy, does for returns to the samsara to prepare.

不过它只能使用一次，一旦戴上后就不能再脱下来，因为灭却师在它的影响下其灵子收束力已经超越了其自身的极限，太过旺盛的火焰最终会烧伤自身，当它再次被解下时，术者将在瞬间得到绝大的力量，但在之后，也将失去其身为灭却师的所有能力压抑言灵：光靠术名就能反射性的发动术牵星箱：死神贵族的象征吧（朽木白哉头上的东西★新断界亚空间，为了不让现世与尸魂界彼此接触而产生的空间虚浮在穿越断界意外失散于轮回外的魂魄，徘徊在断界，经过长年累月聚集在一起，之后魂魄会分离出记忆和能量，做为重返轮回做准备

On the exclamation of the doctor and the cry of the father, the servants all fled with muttered imprecations; they were heard running down the stairs and through the long passages, then there was a rush in the court, afterwards all was still; they had, one and all, deserted the accursed house.

听到医生的绝叫和那父亲的哭喊，仆人们喃喃地祈祷着离开了。只听见他们脚步声奔下楼梯，穿过长廊，冲入前庭，他们都已逃离这座受天诅咒的房子。

Alternatively, the con- trollers can use the synchronous rectifier itself or loss- less inductor current-sensing methods to provide overload protection with lower power dissipation.

另外，康威特罗勒斯可以使用同步整流器本身或亏损减少电感电流检测方法，以提供低功耗过载保护。

Mr. Dauber's other Schatz hangs in his home movie studio.

多伯把沙兹的另一件作品挂在家里的电影室。