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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

There was a picture full of happiness in my heart.i remembered that day,I finished my class after went home by bus,when the bus met red light and stopped there,I looked out of window.i paid attention to a girl of probably three with a long black hair,she gave a sweet kiss to a woman who stand out of balustrade ,after run to sward with a childish smile,and then she lay on the sward and still smile amusedly.

我记得那一天，我下了课就搭车回家，当车碰上了经灯就停了一下，于是我就往窗外看去，注意到一个大约3岁有着黑长头发的小女孩，她亲了一下在栏杆外面的女人，然后就带着天真的笑容跑向草地了，之后他就躺在了草地上，脸上依然都充满开心的笑容。

By using the OP-10/JFC/ amyl alcohol non-ionic compound emulsifier as stabilizer, about 12% organosilicon was appended after the pre-polymerzation of MA, n-BMA, and MAA acrylate monomer was performed and the temperature was decreased to 62℃, and then the pH value was controlled in 7~8 after the polymerization.

结果表明，以OP-10聚醚渗透剂JFC/戊醇非离子复合乳化剂为稳定剂，在MA、n-BMA、MAA丙烯酸酯单体形成预聚体后降温至62℃，加入12%左右的有机硅单体，反应结束后控制pH值7~8，微乳液具有良好的稳定性，乳胶粒具有翻转型的核－壳结构。

The results indicated that amyloplast was filled with starch mostly at 10 days after pollination. Amyloplast developed into mature starch granule at 15 days after pollination. Starch granule arranged tightly with protein body at maturation period.

结果表明：授粉后10 d，淀粉质体几乎完全被淀粉充满；授粉后15 d，淀粉质体已发育为成熟的淀粉粒，成熟期淀粉粒与蛋白质体排列紧密。

"From the day you left , there is no "happiness in my life. After your departure, besides the work, I was crazy in looking for you, even if a little news , I would go and look for you, lays down all most important matters in the hand, but each time I was disappointed and got back. after that day you left, every evening I drawn in the liquor to anaesthetize oneself, I really did not know how should not miss you .

（从你离开的那天起，我的生活再也没有快乐二个字，从你离开的那天起，我除了工作外，就是于处疯狂的找你，哪怕只有关你一点消息，我都会去找你，放下手里所有最重要的事，可是每次都是失望而归，直从你离开的那天起，我每天晚上除了用酒麻醉自己，我真的不知道该如何不去想你。

Causes the image after computer processing, might the greatest degree retention phantom information, after simultaneously optimize the classified phantom, the main use was gains the object analysis situs and the structure information, it certain operations which affected mutually through the object and the structural element, obtained the object essence shape.

使得图像在计算机处理之后，可以最大程度的保留了影像的信息，同时优化了分类后的影像，主要用途是获取物体拓扑和结构信息，它通过物体和结构元素相互作用的某些运算，得到物体更本质的形态。

Result:there are 22 cases in this group ,ratio of occurring is 4.5%. pdge occurred in 19 patients with 6 to 12 days and 1 patients with 5 、14 、15 days after surgery. 20 patients recovered with medical therapy and were discharged by 20 days. an erroneous diagnosis occurred in 2 patients (operated on 1 patient and join together was not narrow.anther 1 was mistaken as part intestines conglutination and block of anastomose gob after gastrectomy.)and also recovered with medical therapy by the extension stay in the hospitals time.

结果：本组共发生功能性排空障碍22例，发生率为4.5%，发生于术后第6--12天共19例，第5天、14天、15天各1例。20例病人经保守治疗20天内痊愈出院，2例因误诊(其中1例再手术，术中未见吻合口梗阻；另1例误诊为胃术后部分肠粘连，吻合口梗阻)延长住院时间亦经保守治疗痊愈出院，胃功能恢复时间平均17.1天。

When be admitted to hospital, mix after be admitted to hospital by clinical nerve function the degree that be short of caustic gives a mark 4 weeks[2] , cent progresses significantly, progress, invariant with death 4 class. 1.2 determine methodological research target (inside the 24h after be admitted to hospital) Yu Qingchen is hollow 4ml of blood of the vein that collect ancon, detached serum - 70 ℃ are saved wait for measure. Hs-CRP reagent is product of German LEADMAN company, day stands - 7600 full automatic biochemical appearance compares chaotic, referenced limits mg/L. Know exactly about sth of place of 1.3 statistical methods is occupied all classics SPSS...

入院时和入院后4周按临床神经功能缺损程度评分[2]，分显著进步，进步、无变化和死亡4级。1.2测定方法探究对象(入院后24h内)于清晨空腹采肘静脉血4ml，分离血清-70℃保存待测。hs-CRP试剂为德国LEADMAN公司产品，日立-7600全自动生化仪比浊，参考范围mg/L.1.3统计学方法所有数据均经SPSS。。。

The downshift of diffraction angle of XRD indicates some precious metals enter crystal lattice of LaMnO3, the crystal sizes of catalysts become larger after precious metal loading; BET and SEM results show sintering happened to catalysts after catalytic reaction.

XRD衍射角向低角度出现了少许偏移，显示负载在表面的贵金属部分进入钙钛矿LaMnO3的晶格结构中，使其晶粒增大；BET和SEM结果表明催化剂在反应后出现了轻微团聚现象。

He described the Ireland after independence, noting that after the Anglo-Irish Treaty, the Irish Free State entered a staunch post colonial phase.

他介绍了爱尔兰独立后，指出后，英爱条约，爱尔兰自由邦进入了一个坚定的后殖民阶段。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。