查询词典 after-hours

We spent about eight to 10 hours at least in bed at the day out of a total of 24 hours.

我们花了大约8至10个小时，至少在床上一天总共有24个小时。

Your bird will need to spend many hours , not just one or two hours , out of the cage with you each day .

您的鸟将需要花费许多时间，而不只是一两个小时与你在笼子外的每一天。

And if you have a retail or food business, the hours you're open may far outstrip the hours it's possible for you to be on location.

如果你有一家零售店或者食品店，你在外的时间会远远超过你在店内的时间。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

In July 6-12, 1982, the population numbers and summer habitat of the Mongolian wild ass Equus hemionus kemionus Pallas were studied by aerial census ing in the Kalamaili Mountains Wildlife Reserve situated in the northeastern part of the Xinjiang Uygur Autonomous Eegion.358 wild asses (0.02 per square km on average) were found by the method of total area census based on the parallel strip transects for conducting aerial census , lasted 32 hours and 21 minutes flying hours These animals separ...

1982年7月航空调查了卡拉麦里山有蹄类野生动物保护区内野驴Equus hemionus hemionus Pallas的夏季栖息地及种群数量。见野驴358头，平均密度为每平方公里0.02头，多集中栖居在保护区的东北部。初步分析影响野驴夏季栖息地的因素是水、地形及人为干扰。

Second, the instructions 1, remote control buttons: 0-9 keys, the red button to reset button,"SET" button to SET for the end,""," CLEAR "key to remove key,"▲"as is"▼ timer starts,"for the countdown began," on "and" pause key for ∨"to" TIME ","MODE" button, the clock display keys,"the timing for 1" for 12 hours system clock,"2" for 24 hours system clock.

二、使用说明 1、遥控器按键说明：0-9数字键、红色键为复位键，"SET"为设置键，""为结束键，"CLEAR"为清除键，"▲"为正计时开始键，"▼"为倒计时开始键，"∧"为暂停键，"∨"为继续键，"TIME"为时钟键，"MODE"为计时显示键，"1*"为时钟12小时制键，"2*"为时钟24小时制键。

Significant superiority over placebo was also observed with respect to most secondary end points: the pain-free rate at 2 hours; the rate of sustained response over a period of 24 hours; the rate of recurrence of headache; improvement in nausea, photophobia, phonophobia, and functional capacity; and the time to meaningful relief.

对 于下列大多数次要终点而言，我们还观察到 BIBN 4096 BS 的疗效明显优于安慰剂：2 小时无痛率；24 小时持续有效率；头痛复发率；恶心，恐光症，恐响症和功能储备的改善；有意义缓解的时间。

For eight hours in a row, she endured the sound of piped music. She gave up and tried again the next day -- and waited another eight hours before putting the phone down.

她在听了八小时的等待音乐后放弃了，并於隔天重拨了一次，她又等了八个小时才挂断电话。

Play up to 5 hours of video or up to 24 hours of audio on a single charge.

播放长达 5小时的视频或长达 24小时的音频，一次充电。

Clinical training requirements: 100 practicum hours, 600 pre-doctoral internship hours, and accompanying course work.

临床培训要求：100小时实习，600博士前的实习时间，工作和相应的课程。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。