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Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同,滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%,显著高于昆明鼠(47.6%);自发激活率分别为13.3%和46.0%,C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%,显著低于SrCl_2孤雌激活率(96.4%);自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%),但囊胚率(18.7%)较低,同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%,差异不显著。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养:自身血清组:分离细胞后原代用含大鼠自身血清的培养基培养,传代后换用含胎牛血清的培养基培养;同种异体血清组:分离细胞后原代用含同种异体血清的培养基培养,以后用胎牛血清培养基培养;胎牛血清组:分离后用含胎牛血清培养基培养,以后一直用含胎牛血清培养基培养;DMEM组:分离细胞后原代用不含血清的DMEM培养基培养,传代后换用含胎牛血清的培养基培养。

Result: the occurrence rate of the chylous fistula after neck dissection was 3.47%; 4 cases of them in operation were cured after ligation in, sewing firm, the ramming of local omohyroid, the chylous fistula after neck dissection did not appear; 6 cases of them after operation was cured by conservative treatment,8 cases were cured through the surgical operation again.

结果:乳糜漏的发生率为3.47%;术中并发乳糜漏4例经手术治疗未出现乳糜漏;术后并发乳糜漏14例中6例经保守治疗治愈,术后8例经再次手术治愈。

The GLDH gene expression showed a continuing reinforcing trend after morphine injection compared with control group, even after drug withdrawal. The ADA mRNA contents in morphine group Ⅰ,Ⅱ and withdrawal group Ⅰ,Ⅱ were 2.65,1.89,1.18, and 0.87-fold of that in control group dividedly. The ADA gene expression increased after morphine injection and fell gradually after withdrawal.

结果:吗啡Ⅰ、Ⅱ组及停药Ⅰ、Ⅱ组大鼠肝脏GLDH mRNA含量分别为对照组的1.02、1.09、1.16及1.46倍,吗啡给药大鼠肝脏中GLDH基因表达随给药时间延长而持续增强,自然停药一定时间内大鼠肝脏中GLDH mRNA含量仍持续增高;吗啡Ⅰ、Ⅱ组及停药Ⅰ、Ⅱ组大鼠肝脏ADA mRNA含量分别为对照组的2.65、1.89、1.18及0.87倍,吗啡Ⅰ、Ⅱ组大鼠肝脏ADA的基因表达均比对照组明显增高,自然停药后大鼠肝脏ADA表达逐渐下降,停药7 d时下降至接近对照组水平。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明:(1)在膜透性的研究中,丛毛羊胡子草抗寒性最强,采用电导率法测得半致死温度LT_(50最低,丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃;在可溶性蛋白含量研究中,抗寒锻炼前后三种植物的可溶性蛋白含量均增加,同时采用SDS-PAGE法,通过对蛋白条带分析,以丛毛羊胡子草条带数最多且着色深;(2)在可溶性糖的研究中,三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高,且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势,丛毛羊胡子草可溶性糖含量下降较小;从游离脯氨酸含量可知,低温的胁迫影响了植物叶片游离脯氨酸的含量变化,植物的抗寒性与游离脯氨酸有关;低温胁迫下三种植物叶绿素含量随着温度的下降,叶绿素含量、叶绿素a、叶绿素b和叶绿素a/b均呈下降趋势,狗牙根对低温胁迫最为敏感;(3)低温锻炼前后,丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的,特别是过氧化物酶,说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

After HRP application to the rostral area (n=10), quite a number of labelled cells were distributed at the ventrolateral part of the nucleus of trapezoid body, the lateroventral periolivary nucleus and the retrotrapezoid nucleus. After HRP application to the caudal area (n=10), HRP labelled cells were mainly located at the lateral reticular nucleus and the ventral part of at the lateral paragigantocellular nucleus LPGi. After HRP application to the intermediate area (n=6), only 8HRP labelled cells were observed at the in 6rats. After HRP application to the control area (n=4), only 6HRP labelled cells were found at the rostral ventrolateral reticular nucleus.

大鼠延髓腹外侧表面的头端化学感受区表面敷贴辣根过氧化物酶后,在延髓斜方体核、外周橄榄腹外侧核和斜方体后核均有HRP标记细胞存在;于尾端化学感受区表面敷贴HRP后,在延髓的巨细胞旁外侧核、外侧网状核和舌下神经核有HRP标记细胞存在;于中间区表面敷贴HRP后,在6只大鼠的延髓内部区域仅观察到8个HRP标记细胞;于对照区敷贴HRP后,在4只大鼠的延髓内部区域仅观察到6个HRP标记细胞,散在地分布在头端腹外侧网状核。

Take the blood specimen to measure the hepatic and renal function and the tissue biopsy after the active and motive abnormities called toxical manifestation such as posture changes, abnormal sound, uneasiness, quiet, thrill, ataxia, convulsion and so on with toxic dose. Measure the tissue biopsy after death with lethiferous dose. If no toxicity or death occur after the experiment of 12 weeks, measure the hepatic and renal function and the tissue biopsy. There was no rat which had the toxical manifestation such as active or motive abnormities and 3 rats died because of environment or others after the experiment of 12 weeks.

实验中分别测定治疗剂量组小鼠在用药4周,8周,12周后的肝肾功能,并进行组织病理检查;中毒剂量组小鼠在出现行为、动作异常,如改变姿势、叫声异常、不安、安静、震颤、运动失调、惊厥等中毒表现时,取血液标本测定肝肾功能,并进行组织病理检查;致死剂量组小鼠发生死亡后进行组织病理检查;若12周实验结束时无小鼠出现中毒表现或死亡,则取血液标本测定肝肾功能,并进行组织病理检查。12周实验结束时,无小鼠出现动作、行为异常等中毒表现,有3只小鼠因为环境或其它原因发生死亡,病理检查结果正常。

Luchuan also said that among young people the film also won the praise: A lot of the audience are, after'80 ','90 after', but after reading the After the film was very excited to give our views, I also express their views on this period of history.

陆川更表示,这部电影在年轻人当中也赢得了好评:很多观众都是'80后'、'90后',但是看完电影之后非常激动,给我提意见也是发表自己对这段历史的看法。

Various kinds of lumbodynia amended diversity extently through the flavone of rhizome drynaria treated after 3 months. After 1 month, the effect of lumbodynia on sleep is extremely trifling; after 2 months, the majority of rest and prolonged sitting pains nearly vanished; after 3 months, the majority of pains while to turn the body over/to get up/bend from the waist/tenderness/percussion pain can amend, but a piece of patients still showed the pain that bend from the waist and the percussion pain.

运用骨碎补总黄酮治疗3个月后,各种行为方式引起的腰背痛均有不同程度的改善,其中治疗1个月后腰背痛对睡眠的影响较小,治疗2个月后多数静止痛、久坐痛基本消失,治疗3个月多数翻身痛、起身痛、步行痛、压痛可缓解,但部分病人仍然呈现出一定程度的弯腰痛和腰背叩击痛。

Results: Of the 22 cases, the draining veins, which were observed through MR brain surface anatomy scanning, were highly coincidence with the findings in the operation. The myodynamia of the one case whose myodynamia was grade 0 preoperation was grade IV one month after operation, the one case whose myodynamia was grade II preoperation was grade IV two weeks after operation, the one case whose myodynamia was grade III preoperation was grade IV three months after operation, and the patient recovered normal eight months later, the two cases whose myodynamia was grade IV total recovery after 2 months .

结果:22例患者的术前磁共振脑表面成像中脑表面回流静脉与术中所见高度一致。1例肌力0级患者术1月恢复至 IV 级,1例 II 级患者术后2周恢复至 IV 级,1例 III 级患者术3月恢复至 IV 级,随访8月患者对侧肢体肌力恢复正常,2例 IV 级患者术后2月完全恢复。

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相关中文对照歌词
After All It's Christmas
Ever After
Gimme Gimme Gimme (A Man After Midnight)
After The Last Midtown Show
Walking After Midnight
Fifty Years After The Fair
After All
After 17
After Ever After
Always After You ('Til You Started After Me)
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Do you know, i need you to come back

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