查询词典 after-class

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

Companies to adopt direct marketing system and after-sales service system, is designed to let customers get the best price and best after-sales service.

公司采取直接营销体系和售后服务体系，目的是为了让客户得到最优的价格和最完善的售后服务。

After 30 days, the program will be disabled after 15 minutes of use.

30天之后，该计划将被禁用15分钟后使用。

Because the Taiwan educational system enters a higher school disadvantageously to the Taiwan student and so on the reason, creates goes to the Taiwan student which Japan studies abroad to be more and more many, especially after 1920 has reveals the growth, after in addition foreign student Western world's individualistic thinking influence, gradually has the change to the marital idea, from marries for the family to chooses the object for oneself the transformation, let a this factor naturally facilitate " Japanese and Taiwanese get marriage each other ".

因台湾教育制度对台湾学生升学不利等原因，造成赴日留学的台湾学生越来越多，尤其在1920年之后更有显著的成长，加上留学生受西洋的个人主义思想影响之后，对婚姻观念逐渐有所改变，从为家而结婚到为自己选择对象的转变，让此一因素自然而然地促成了「内台共婚」。

The incidence of early epileptic seizures after intracerebral hemorrhage was high. The cortical hemorrhage and the medical history of intracerebral hemorrhage, electrolyte disbalance were the important related factors for the incidence of early epileptic seizures after intracerebral hemorrhage. The cortical hemorrhage was the independent risk factor.

脑出血后早发性癫痫发作的发生率较高，皮质出血、脑出血史以及电解质紊乱是影响脑出血后早发性癫痫发作的重要因素，皮质出血为独立的危险因素。

One feels annoyed after listening to it, feels sick after watching it, feels disburden not to buy it, and feels safe not to use it.

听了烦心，看了恶心，不买放心，不用安心，这道四"星"级的菜肴是什么？

One feels annoyed after listening to it, feels sick after watching it, feels disburden not to buy it, and feels safe not to

听了烦心，看了恶心，不买放心，不用安心，这道四"星"级的菜肴是什么？

One feels annoyed after listening to it, feels sick after watching it, feels disburden not to buy it, and feels safe not touse it.

听了烦心，看了恶心，不买放心，不用安心，这道四"星"级的菜肴是什么？

One feels annoyed after listening to it, feels sick after watching it, feels disburden not to buy it, and feels safe not to use it. What's

听了烦心，看了恶心，不买放心，不用安心，这道四"星"级的菜肴是什么？

One feels annoyed after listening to it, feels sick after watching it, feels disburden not to buy it, and feels safe not

听了烦心，看了恶心，不买放心，不用安心，这道四"星"级的菜肴是什么？

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。