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［Objective］ To analyze the outcome of internal fixation for occipitalization with atlantoaxial joint dislocation by posterior decompression and occipitocervical fusion ［Method］ From December 2005 to June 2007,8 patients with occipitalization and atlantoaxial joint dislocation received removal of the posterior arcus of atlas and the enlargement of the posterior edge of the foramen magnum after skull traction performing for an average of 135 daysAll patients were operated on by posterior craniocervical fusion using cervifix internal fixation system and autologous ilium graftsThe clinical efficacy after operation was analyzed by Japanese Orthopaedic Associationneural function score ［Result］ All the patients were followed up from 6 months to 2 years, average of 15 monthsNo complication was foundAtlantodental interval was 5~9 mm before and 4~6 mm after skull tractionAtlantoaxial joint dislocation didn't completely reducedThe neurological defects were improved to some extents according to the JOA scoreImageology showed all patients had full decompression and bony fusionThe loosening or broken internal fixation was not found ［Conclusion］ Posterior decompression and fusion is a feasible method for the treatment of occipitalization with atlantoaxial joint dislocation,and the clinical effect is satisfactory

分析后路减压枕颈融合内固定术治疗合并寰枢关节脱位的寰椎枕骨化临床疗效。［方法］2005年12月至2007年6月间，对8例合并寰枢关节脱位的寰椎枕骨化患者在行颅骨牵引治疗一段时间（12~16 d，平均135 d）后采用枕骨大孔后缘扩大，寰椎后弓切除减压取自体髂骨枕颈融合Cervifix系统内固定术，手术后采用日本骨科学会神经功能评分分析临床疗效。［结果］8例患者随访6个月~2年，平均为15个月。8例患者无一例出现术后并发症，术前寰齿前间隙为5~9 mm，经颅骨牵引后为5~7 mm，寰枢关节脱位未能完全复位。手术前后JOA评分示神经症状均有不同程度恢复，影像学检查示枕颈区减压充分植骨区获得骨性融合，无一例出现内固定松动或断裂。［结论］合并寰枢关节脱位的寰椎枕骨化患者术前仔细评估影像学改变，采用颅骨牵引一段时间后行后路减压枕颈融合内固定术的治疗方案是合理可行的，且临床效果满意。

The changing trend of progesterone during oestrous cycle resembles with that during the first 14d of early pregnancy. Its content reaches the peak value at lOd after oestrus and then declines. The estradiol peak appears at 3d of oestrous cycle, and the level rises again before oestrus. The concentration of LH declines after oestrus. The level of follicle stimulating hormone reaches the peak value at lOd after oestrus (2.40mIU/ml).

发情周期：民猪发情周期孕酮的含量与妊娠早期14天内的变化极其相似，在发情后第10天达峰值，而后开始下降；雌二醇水平在发情后第3天出现高峰，下次发情前开始升高；促黄体素在发情当天达最高水平，然后开始下降；促卵泡素在发情后的第10天达到峰值(2.40mIU/ml)。

Through comparing and analyzing the photographs of Cephalometrics before orthodontics, after orthodontics and two years after orthodontics, we can probe into the optimal period for treating AngleⅢmalocclusion osteal cross bite patients with maxillary protraction therapy and the long period of stability after the accomplishment of orthodontics, and it can provide reference and theoretics for clinic practice and experiments.

通过对不同年龄组患者矫治前、矫治结束时及矫治结束两年后的头颅侧位片进行定点、描绘、测量、比较分析，进而探讨上颌前方牵引治疗安氏Ⅲ类骨性反牙合的最佳矫治时机以及矫治结束后的长期稳定性，为进一步的临床实践与实验研究提供理论依据。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

In Perclose group and Angio-Seal group, the punctured sites in femoral arteries were sealed immediately after CAG and/or PCI. In manual compression group, the punctured sites in femoral arteries were compressed by hands imme- diately after CAG or 4-6 hours after PCI.

冠脉诊疗术后Perclose组、Angio-Seal组经股动脉造影后，分别用Perclose血管缝合器、Angio-Seal血管封堵器封闭股动脉穿刺点；手工压迫组行单纯CAG后立即手工压迫股动脉止血，PCI术后4～6h手工压迫股动脉止血。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

Of applications to clean ·马 Ruyou Caiqingchong Pieris rapae, the field of medicinal test, the test results showed : 20% of clean ·马 Ruyou Caiqingchong have good preventing and controlling effect, albeit one day after 1500 Beiye, 2000 Beiye, 2500 amounted to 96.45% Beiye-effective, 91.94%,89.45%; medicine after three days of anti-drug effect after seven days, respectively, to 99.02%,95.85%,92.94%; 98.56%,92.62,87.42% respectively.

应用20%氰·马乳油防治菜青虫Pieris rapae的田间药效试验，试验结果表明：20%氰·马乳油对菜青虫具有良好的防治效果，施药后1天1500倍液，2000倍液，2500倍液防效达96.45%，91.94%，89.45%；药后3天的防效分别达99.02%，95.85%，92.94%；药后7天分别为98.56%，92.62，87.42%。

objective to explore the related factors of recurrence of chronic subdural hematoma after neurosurgery.methods the related aspects that affected the recurrence in 113 cases with chronic subdural hematomas after neurosurgery were reviewed,and the factors were evaluated by the medical literature.results 5 patients recured after neurosurgery,and 1 patient died.rate of recurrence and death were 4.4% and 0.9% respectively.conclusion age,abnornmal blood coagulation mechanism,density,pneumatosis and drainage volume would affect the prognosis.

目的 探讨影响慢性硬膜下血肿（chronic subdural hematoma，csdh）术后复发的因素。方法回顾2003～2004年本院手术治疗的113例csdh患者，并结合有关文献进行相关分析。结果术后复发5例，死亡1例，复发率和死亡率分别为4.4%和0.9%。复发的5例患者平均年龄69.6岁。其中因服用抗凝剂复发1例，因术后大量颅内积气复发1例，高密度复发1例，等密度到混杂密度复发2例。结论患者的年龄、凝血机制异常、血肿的密度、术后积气和引流量的多少影响csdh患者的预后。

This article after 80 after basic creation characteristics, the network will be the opportunity as well as the market impetus which 80 writes supplies portably affects three aspects to obtain, after analyzes 80 literatures the reason which is all the rage in the literature market.

本文将从80后的基本创作特征、网络为80后写手提供的机遇以及市场的推动作用三个方面入手，分析80后文学在文学市场上风靡的原因。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。