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Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用,并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1),另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死,取出损伤节段脊髓用来组织病理学检查,同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过,到损伤后28天,我们观察发现,损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18,group 1∶9.89±1.29;p.05),光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

The results of changes of endogenous hormone contents at early stage of floral differentiation in cotyledonary nodes of cucumber seedlings after decapitation were as follows: The contents of IAA and ZT in cotyledonary nodes of the cucumber seedlings within 0~4 d after decapitation decreases while the level of GA3 first increases then declines within 1~3 d, after that it increases again.

对花芽分化初期子叶节中内源激素质量分数的测定表明,IAA质量分数在花芽分化初期处於较低水准,ZT质量分数呈下降趋势,GA3质量分数则出现升-降-升的波动状态。

The toxicity of trichlorphon would become degradation after a week under the same conditions. For example, the survival rate of Daphnia pulex reached to 100 percent in the concentration of 200μg/L after 144h and 65 percent in the concentration of 400μg/L after 168h.

在相同的实验条件下,敌百虫溶液的毒性可在一周后降解:如200μg/L的敌百虫溶液在放置144h后,蚤状溞的存活率就达到100%;168h后,400μg/L的存活率为65%。

In order to minimize the postoperative complications of uvulopalatopharyngoplasty for preventing severe obstructive sleep apnea syndrome, the data of 32 patients with severe OSAS undergoing UPPP were analyzed. Three cases got temporary regurgitation after UPPP. Ten days to 2 months after UPPP, all patients except 4 obtained satisfactory therapeutic effects after decannulation. All the patients were decannulated favorably.

摘 要:为预防阻塞性睡眠呼吸暂停综合征行悬雍垂腭咽成形术时的并发症,对32例OSAS患者作预防性气管切开术行UPPP治疗的经验进行总结,UPPP术后,3例出现短暂进食返流,无其它并发症发生,术后10d~2个月,所有患者均拔管顺利,28例疗效明显,4例自觉症状无改善。

Its test result, after using agent of sewage disposal of biology of carapace god ocean and technology of flood body rehabilitate, build good oxygen biology to stabilize a bed, processing sewage effect is apparent, expression is: Black smelly sewage is rapid decolour, deodorization, become clear, insipidity, the COD after handling two days by 333 drop 76, achieve view and admire water and the water quality demand that breed water, can develop aquatic substance. A green alga, red snapper grows to feed after 4 days wait for primary live thing, breed the water biology such as water hyacinth to be able to put in a suitable place to breed view and admire a fish.

其试验结果,运用甲壳神海洋生物污水处理(通过物化方法去除水中一些物质的过程)剂和大水体修复技术后,建立好氧生物稳定床,处理污水效果明显,表现为:黑臭的污水迅速脱色、脱臭,变得澄清、无味,处理两天后COD由333下降到76,达到观赏水和养殖水的水质要求,可培养水生物。4天后长出绿藻、红鱼食等初级生物,养殖水葫芦等水生物并可放养观赏鱼。

Took dephlogisticate pain-killer orally after operation and undertook functional exercise from the first day after operation. Dermal sutured out at the fourteenth day after operation and kept on functional exercise for one week.

术后常规口服消炎镇痛药,第1天开始进行功能锻炼,术后14天拆线,拆线后继续功能锻炼1周,待膝关节屈伸活动度较稳定,不易反弹后方可出院,并继续行功能锻炼。

Results Non-specific antibody increased distinguishingly in each groups after drug treatment 30 days, but all tested indexes showed no anychange in 2mg/ group. Glomerular basement membrane became thick in 10mg/ group after drug treatment 90 days, but it showed normal when treatment time was shorten to 30 days. The thickness of glomerular basement membrane was very serious in in 50mg/ group and liquid containing protein was found increasing in glomerular and tubular. The thickness of glomerular basement membrane didn't ameliorate after stopping drug 30 days.

结果 给药后30天各给药组动物血清非特异性抗体明显升高。rhIL-1Ra 2mg/组其他检测指标均未见明显地改变。rhIL-1Ra 10mg/组给药后90天肾小球毛细血管基底膜明显增厚,但此剂量给药时间为30天时未见任何异常。rhIL-1Ra 50mg/组肾小球及肾小管中蛋白性液体量多,小球毛细血管基底膜增厚更为严重,且停药30天后基底膜增厚程度仍未见明显减轻或改善。

The formation of charge transfer complex was studied in the first part. The second part includes the synthesis and characterization of the acrylated unsaturated polyester and the kinetic studies on the Ar-UPE/ triethylene divinyl ether (DVE-3) photocuring system, the rheology and properties of film after photocuring. The third part includes the synthesis and characterization of the urethane modified unsaturated polyester and the kinetic studies on the PU-UPE/DVE-3 photocuring system, the rheology and properties of film after photocuring. The fourth part includes the synthesis and characterization of the vinyl ether end capped polyurethane and the kinetic studies on the VE-PU/DVE3 photocuring system, the rheology and properties of film after photocuring.

整个研究工作分为四个部分:一是电荷转移复合物的研究;二是丙烯酰氧基化不饱和聚酯的合成,表征,Ar-UPE/二缩乙二醇二乙烯基醚(DVE-3)体系光固化动力学研究以及Ar-UPE/DVE-3体系流变性能和光固化后涂膜综合性能的研究;三是氨酯改性的不饱和聚酯的合成,表征,PU-UPE/DVE-3体系光固化动力学研究以及该体系光固化后涂膜综合性能的研究;四是乙烯基醚封端的聚氨酯的合成,表征,VE-PU/马来酸二甲酯体系光固化动力学研究以及VE-PU/DMA体系流变性能和光固化后涂膜综合性能的研究。

CD8~+ cell is the main T lymphocyte subset in spleen, and B lymphocyte mainly is IgG~+ cell, moreover the amount of these B lymphocytes could exceed CD3~+ T lymphocyte subset after 7 days. CD8~+ cell is the main T lymphocyte subset in tonsil of appendix, and B lymphocyte is IgM~+ cell, and the amount could exceed CD3~+ T lymphocytes after 35 days. After 21 days, B lymphocytes in esophago tonsil are the main IgA~+ cells and the amount exceeds CD3~+ lymphocytes. The amount of CD4~+ lymphocytes is more than CD8+ lymphocytes.4. CD3~+、CD4~+ and CD8~+ T lymphocytes in spleen mainly distribute in periarterial lymphoid sheath. However IgM~+、IgG~+ and IgA~+ cells mainly distribute in ellipsoid periarterial lymphoid sheath and germinal center. T lymphocytes in appendix tonsil mainly distribute in middle and inferior part of mucous and the B lymphocytes mainly in middle and mucous between 4~7 days. Whereafter T, B lymphocytes equably distribute in mucous. CD4~+ cells arrange tightly and mainly occupy the central part in aggregates of T lymphocytes in esophago tonsil and CD8~+ lymphocytes mainly distribute in periphery. Meanwhile B lymphocytes encircle the periphery of aggregates of T lymphocytes. The aggregates of B lymphocytes is mainly the germinal center with lots of IgM~+、IgG~+ and IgA~+ cells. Meanwihle T lymphocytes encircle the periphery of aggregates of B lymphocytes.5. There is an intimate relationship between the development of tissue structure of peripheral immune organs and lymphcytopoiesis. The maturation of tissue structure is stimulated by the immigration of lymphocytes and the mature tissue structure provides place where lymphocytes grow mature and functionate.

脾脏在21日龄时达到稳定,食管扁桃体和盲肠扁桃体均在35日龄时达到稳定;脾脏中T淋巴细胞亚群以CD8~+细胞为主,B淋巴细胞则以IgG~+细胞为主,并在7日龄后数量超过CD3~+T淋巴细胞;盲肠扁桃体中T淋巴细胞亚群以CD8~+细胞为主,B淋巴细胞以IgM~+细胞为主,并在35日龄后数量超过CD3~+T淋巴细胞;21日龄后,食管扁桃体中B淋巴细胞以IgA~+细胞为主,数量超过CD3~+细胞,CD4~+细胞的数量多于CD8~+细胞。4、在T、B淋巴细胞组织定位方面,脾脏中CD3~+、CD4~+和CD8~+T淋巴细胞主要分布在动脉周围淋巴鞘中,而IgM~+、IgG~+和IgA~+细胞主要分布在椭球周围淋巴鞘和生发中心中;4~7日龄时,盲肠扁桃体中T淋巴细胞主要分布在粘膜固有层的中下部区域,而B淋巴细胞则主要分布在中上部区域,随后各日龄T、B淋巴细胞均匀地分布在粘膜固有层中;在食管扁桃体的T淋巴细胞聚集物中,CD4~+细胞紧密排列,主要占据中央部位,CD8~+细胞主要散布在外周,同时B淋巴细胞又环绕在整个T淋巴细胞聚集物的外周;B淋巴细胞聚集物主要为生发中心,其中存在大量IgM~+、IgG~+和IgA~+细胞,同时T淋巴细胞又环绕在整个B淋巴细胞聚集物的外周。5、外周免疫器官的组织结构发育和淋巴细胞发生之间存在密切的关系,淋巴细胞迁入淋巴器官刺激组织结构的发育成熟,同时成熟的组织结构又为淋巴细胞发育成熟并行使功能活动提供场所。

The unit of radioreaction in ethmoidal sinus was ethmoidal cell. Several ethmoidal sinus cells were fused to patch in severe cases. The radioreactions in ethmoidal sinus, maxillary sinus and sphenoidal sinus were seen within three months, after three months and after one year respectively. And nasal sinus radioreactions of most cases were not absorbed spontaneously after one year. When the two kinds of radiotherapies were equal in dosing level, there was no obvious difference between them in respect to the radioreaction of accessory sinus, but when the dosing level was above 3182GBq, the radioreaction of accessory sinus was remarkably increased, compared with those caused by lower-dose radiotherapies.

筛窦放射反应以筛窦小房为单位,重者也可以多个小房融合成片状;筛窦的放射反应在放疗后3个月内即可出现,上颌窦常在3个月以后出现放射反应,而蝶窦一般在放疗后1年以后才出现;大多数病例副鼻窦放射反应在1年以后难以自动吸收、消退;两种方法放疗剂量相同时所致副鼻窦放射反应无明显差别,但放疗剂量在3182 GBq以上时所致副鼻窦放射反应较其它放疗剂量时明显增高。

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