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MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The bcl-2/bax of Safflor group treated before ICH 24 hours ahead,after ICH 0 hour were lower than that of Safflor group treated after ICH 6,12 or 24 hours. When the treated group were injected Safflor after ICH 6,12,24 hours,The number of caspase-3 positive cell around

在不同时间给予红花注射液治疗脑出血大鼠，均能不同程度地促进脑出血大鼠神经功能的恢复，其中术后6h给药组治疗效果最好，其可能通过促进Bcl-2蛋白的表达，抑制Bax和Caspase-3蛋白的表达，从而抑制血肿周围的细胞凋亡。

When the treated group were injected Safflor after ICH 6,12,24 hours,The number of caspase-3 positive cell around hematoma was gradually increased and the number of bcl-2/bax positive cell was gradually degraded and the number of TUNEL positive cell of Safflor group treated after ICH 6 hours were less than that of Safflor group treated after ICH 24 hours.

在不同时间给予红花注射液治疗脑出血大鼠，均能不同程度地促进脑出血大鼠神经功能的恢复，其中术后6h给药组治疗效果最好，其可能通过促进Bcl-2蛋白的表达，抑制Bax和Caspase-3蛋白的表达，从而抑制血肿周围的细胞凋亡。

The above facts indicated that silicon accumulation in the rice leaf sheath inside, the leaf surface and the sclerenchymatous cell, played the physical barrier role, delayed the hypha of sheath blight expansion.5、Analysing the physiological and biochemical mechanism of the resistance to rice sheath blight improved by silicon application in several respects.(1) After being inoculated with R.solani, O2 and POD activities disordered, CAT activities obviously declined. O2 producingvelocity in 91SP\'s leaves and leaf sheaths of Si+ rice plants were lower than those of Siriceplants, MDA content in leaves and leaf sheaths of Si+ rice plants were lower thanthose of Si- rice plants significantly in three days after inoculating, SOD、POD、CATactivities in leaf sheaths of Si+ rice plants were higher than those of Si- rice plantssignificantly in one day after inoculating, SOD、POD、CAT activities in leaves ofSi-treatment were higher than those of Si- rice plants significantly in three days afterinoculating. O2 producing velocity and MDA content in Lemont\'s leaves and leafsheaths of Si+ rice plants were lower than those of Si- rice plants significantly afterinoculating, and SOD、POD、CAT activities of Si+ rice plants were higher than those ofSi- rice plants significantly.

说明硅在水稻叶鞘内侧、叶表和厚壁细胞积累，起到了物理屏障的作用，延缓了纹枯病菌的扩展。5、阐明了施硅增强水稻抗纹枯病的生理生化机制(1)接种纹枯病菌后，两个水稻品种91SP和Lemont的叶鞘和叶片超氧阴离子自由基O2性紊乱，CAT活性明显下降；抗病品种91SP叶鞘和叶片施硅处理的O2产生速率小于缺硅处理，叶鞘和叶片MDA含量在接种第3 d后显著低于缺硅处理，叶鞘SOD、POD、CAT活性在接种1 d后一直显著高于缺硅处理，叶片SOD、POD、CAT活性在接种3 d后显著高于缺硅处理；感病品种Lemont叶鞘和叶片施硅处理的O2产生速率、MDA含量始终显著小于缺硅处理，SOD、POD、CAT活性始终显著大于缺硅处理。

Methods:(1) The two-sided ovary of female animal and the two-sided testicle of male animal were excised. After the XLGBC to treat 28 days, the estradiol and the teststerone of serum were checked;(2) With intramuscular injection the Hydrocortisone,the Yangxu animals was made. After the XLGBC to treat 10 days, the testicle coefficient,the spermathecal gland coefficient and the ovary coefficient were observed;(3) With ig the Thyroid tablets and the Reserpine, the Yinxu animal was made. After the XLGBC to treat 10 days, the testicle coefficient,the spermathecal gland coefficient and the ovary coefficient were observed.

（1）雌性动物摘除双侧卵巢，雄性动物摘除双侧睾丸，复制成去势动物模型后予仙灵骨葆胶囊治疗28天，检测血清雌二醇和血清睾酮；（2）肌注氢化可的松，复制成阳虚动物模型后予仙灵骨葆胶囊治疗10天，观测并计算动物的睾丸系数、精囊腺系数和卵巢系数；（3）灌胃甲状腺片和利血平，复制成阴虚动物模型后予仙灵骨葆胶囊治疗10天，观测并计算动物的睾丸系数、精囊腺系数和卵巢系数。

Results One patient was complicated with prilnary non-function of the transplanted liver after liver transplantation;Five patients completed regained consciousness within 48 hours after operation; One patient complicated with acute renal failure;One patient complicated with acute liver cell rejection postoperatively and reversed after treatment;One male patient complicated with serious stomatocace;Two patients complicated with acute respiratory distress syndrome,underwent tracheotomy.

结果行急诊肝脏移植治疗6例，术后1例并发原发性移植肝无功能，5例患者于48h内苏腥。1例男性患者术后并发急性肾功能衰竭，1例患者术后发生排斥反应，经治疗后逆转。1例男性患者并发口腔严重溃疡。2例并发急性呼吸窘迫综合征，行气管切开。

Results compared with normal group and sham operated control groups, brdu positive and nestin positive cells were increased in the hippocampus, dentate subgranular zone and subependymal zone in operated groups after 24 hours of reperfusion of omni-cerebral ischemia. the expression of brdu and nestin reached a peak 7-10 days after the operation, and could still be detected 20 days after the operation. the migration of brdu positive and nestin positive cells to the cerebral cortex and hippocampus was observed in subependymal region.

结果 脑缺血再灌流24 h后，海马、齿状回和室管膜下区的brdu阳性细胞和nestin阳性细胞增多，7～10 d达到高峰，术后20 d仍有表达；在室管膜下区，brdu阳性细胞和nestin 阳性细胞有向皮质、海马迁移的现象。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Methods Emergency endoscope ex-amination were carried out in20massive hemorrhage clients after gastrectomy and esophagectomy,after exposed and washed the bleeding part by cold0.9%NS,1∶10000adrenaline and hypertonic saline were injected in perifocal sub-mucosa.After stop bleeding and before move back the endoscope,spray some YunNanBaiYao and thrombase in the bleeding part to prevent secondary hemoorahge.

对食管、胃切除术后引起大出血的患者行急症内镜检查，用冰冻生理盐水100ml+去甲肾上腺素8mg反复冲洗，暴露出血部位后在病灶周围黏膜下注射1∶10000肾上腺素及高渗盐水，无出血后，退镜前于出血部位喷洒云南白药及凝血酶，防止再次出血。

Methods Emergency endoscope examination were carried out in 20 massive hemorrhage clients after gastrectomy and esophagectomy, after exposed and washed the bleeding part by cold 0.9% NS, 1:10000 adrenaline and hypertonic saline were injected in perifocal sub mucosa. After stop bleeding and before move back the endoscope, spray some YunNanBaiYao and thrombase in the bleeding part to prevent secondary hemoorahge.

对食管、胃切除术后引起大出血的患者行急症内镜检查，用冰冻生理盐水lOOml＋去甲肾上腺素8mg反复冲洗，暴露出血部位后在病灶周围黏膜下注射1：10000肾上腺素及高渗盐水，无出血后，退镜前于出血部位喷洒云南白药及凝血酶，防止再次出血。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新