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The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

By the accurate appraisal of 16sDNA,confirmed it as Bacillus subtilis of Bacillus Cohn. Measured the biological activity:the strain entered into the stationary stage after be cultivated after 18 hours ,and after 30 hours entered into the contabescence stage.

将该菌株进行形态学特征、生理生化特征等分析，以及16sDNA的鉴定，确定为芽孢杆菌属，枯草芽孢杆菌。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

The results of changes of endogenous hormone contents at early stage of floral differentiation in cotyledonary nodes of cucumber seedlings after decapitation were as follows: The contents of IAA and ZT in cotyledonary nodes of the cucumber seedlings within 0~4 d after decapitation decreases while the level of GA3 first increases then declines within 1~3 d, after that it increases again.

对花芽分化初期子叶节中内源激素质量分数的测定表明，IAA质量分数在花芽分化初期处於较低水准，ZT质量分数呈下降趋势，GA3质量分数则出现升-降-升的波动状态。

The toxicity of trichlorphon would become degradation after a week under the same conditions. For example, the survival rate of Daphnia pulex reached to 100 percent in the concentration of 200μg/L after 144h and 65 percent in the concentration of 400μg/L after 168h.

在相同的实验条件下，敌百虫溶液的毒性可在一周后降解：如200μg/L的敌百虫溶液在放置144h后，蚤状溞的存活率就达到100％；168h后，400μg/L的存活率为65％。

In order to minimize the postoperative complications of uvulopalatopharyngoplasty for preventing severe obstructive sleep apnea syndrome, the data of 32 patients with severe OSAS undergoing UPPP were analyzed. Three cases got temporary regurgitation after UPPP. Ten days to 2 months after UPPP, all patients except 4 obtained satisfactory therapeutic effects after decannulation. All the patients were decannulated favorably.

摘 要：为预防阻塞性睡眠呼吸暂停综合征行悬雍垂腭咽成形术时的并发症，对32例OSAS患者作预防性气管切开术行UPPP治疗的经验进行总结，UPPP术后，3例出现短暂进食返流，无其它并发症发生，术后10d～2个月，所有患者均拔管顺利，28例疗效明显，4例自觉症状无改善。

Its test result, after using agent of sewage disposal of biology of carapace god ocean and technology of flood body rehabilitate, build good oxygen biology to stabilize a bed, processing sewage effect is apparent, expression is: Black smelly sewage is rapid decolour, deodorization, become clear, insipidity, the COD after handling two days by 333 drop 76, achieve view and admire water and the water quality demand that breed water, can develop aquatic substance. A green alga, red snapper grows to feed after 4 days wait for primary live thing, breed the water biology such as water hyacinth to be able to put in a suitable place to breed view and admire a fish.

其试验结果，运用甲壳神海洋生物污水处理(通过物化方法去除水中一些物质的过程)剂和大水体修复技术后，建立好氧生物稳定床，处理污水效果明显，表现为：黑臭的污水迅速脱色、脱臭，变得澄清、无味，处理两天后COD由333下降到76，达到观赏水和养殖水的水质要求，可培养水生物。4天后长出绿藻、红鱼食等初级生物，养殖水葫芦等水生物并可放养观赏鱼。

Took dephlogisticate pain-killer orally after operation and undertook functional exercise from the first day after operation. Dermal sutured out at the fourteenth day after operation and kept on functional exercise for one week.

术后常规口服消炎镇痛药，第1天开始进行功能锻炼，术后14天拆线，拆线后继续功能锻炼1周，待膝关节屈伸活动度较稳定，不易反弹后方可出院，并继续行功能锻炼。

Results Non-specific antibody increased distinguishingly in each groups after drug treatment 30 days, but all tested indexes showed no anychange in 2mg/ group. Glomerular basement membrane became thick in 10mg/ group after drug treatment 90 days, but it showed normal when treatment time was shorten to 30 days. The thickness of glomerular basement membrane was very serious in in 50mg/ group and liquid containing protein was found increasing in glomerular and tubular. The thickness of glomerular basement membrane didn't ameliorate after stopping drug 30 days.

结果 给药后30天各给药组动物血清非特异性抗体明显升高。rhIL-1Ra 2mg/组其他检测指标均未见明显地改变。rhIL-1Ra 10mg/组给药后90天肾小球毛细血管基底膜明显增厚，但此剂量给药时间为30天时未见任何异常。rhIL-1Ra 50mg/组肾小球及肾小管中蛋白性液体量多，小球毛细血管基底膜增厚更为严重，且停药30天后基底膜增厚程度仍未见明显减轻或改善。

The formation of charge transfer complex was studied in the first part. The second part includes the synthesis and characterization of the acrylated unsaturated polyester and the kinetic studies on the Ar-UPE/ triethylene divinyl ether (DVE-3) photocuring system, the rheology and properties of film after photocuring. The third part includes the synthesis and characterization of the urethane modified unsaturated polyester and the kinetic studies on the PU-UPE/DVE-3 photocuring system, the rheology and properties of film after photocuring. The fourth part includes the synthesis and characterization of the vinyl ether end capped polyurethane and the kinetic studies on the VE-PU/DVE3 photocuring system, the rheology and properties of film after photocuring.

整个研究工作分为四个部分：一是电荷转移复合物的研究；二是丙烯酰氧基化不饱和聚酯的合成，表征，Ar-UPE/二缩乙二醇二乙烯基醚（DVE-3）体系光固化动力学研究以及Ar-UPE/DVE-3体系流变性能和光固化后涂膜综合性能的研究；三是氨酯改性的不饱和聚酯的合成，表征，PU-UPE/DVE-3体系光固化动力学研究以及该体系光固化后涂膜综合性能的研究；四是乙烯基醚封端的聚氨酯的合成，表征，VE-PU/马来酸二甲酯体系光固化动力学研究以及VE-PU/DMA体系流变性能和光固化后涂膜综合性能的研究。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新