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After the cells were treated by high concentration toxin for 4d, the content of nitrate-nitrogen in cells were lower markedly than the contrast, after the cells were treated by middle and low concentration toxin for 7d, the content of nitrate-nitrogen in cells were lowered. 3 groups of toxin treating all lowered the total phosphorus content. At the end of test, the phosphorus contently of low, middle, high concentration treating groups were 74.98%, 76.47% and 84.00% of the contrast respectively. The ACP activity of 3 treating groups compared with the contrast appeared the tendency of first lowering then raising.

高浓度毒素处理细胞4d后，细胞内硝态氮含量显著低于对照；中、低浓度毒素处理细胞7d后降低了胞内硝态氮含量。3组毒素处理均降低了胞内总磷含量，到实验结束时，低、中、高浓度处理组的胞内磷含量分别为对照的74.98%、76.47%和84.00%。3组处理组ACP活力与对照相比呈现先降低后升高的趋势。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

The power circuit passes through the live pressure, the rectification, after the filter, after three end integration manostat 7805 output stable 5V DC voltage, takes program timer's main power source.

电源电路经变压，整流，滤波后经三端集成稳压器7805输出稳定的5V直流电压，作为程序控制器的主电源。

The side effects in two groups were observed.Results 3~7 days after the end of treatment,the cure rate and efficacy rate were 64.3%and 82.5%in control group and 76.2%and 91.5%in trial group,respectively.3~7 days after menorrhea,the cure rate andefficacy rate were 65.9%and 83.3%in control group and 78.5%and 92.3%in trial group,respectively.There was a significant difference between two groups.

结果 停药后3~5 d，对照组的治愈率和有效率分别为64.3%和82.5%，试验组的治愈率和有效率分别为76.2%和91.5%；停药后下次月经来潮干净后3~7 d对照组的治愈率和有效率分别为65.9%和83.3%，试验组的治愈率和有效率分别为78.5%和92.3%；两组比较有显著性差异(P.05)。

Forty healthy pigment rabbits without oculopathy were divided into 8 groups, 1 hour, 3 hours, 1 day, 3, 7, 14, 28 days after retinal detachment and normal control, 5 rabbits in each group. Right eye of each rabbit was treated with infusing sodium hyaluronate under retinal neuroepithelium layer. The eyeballs were enucleated at different times after RD. The morphologic changes of the retina were observed by electron microscopes. Terminal deoxynucleotidyl transferasemediated dUTP nick end labeling technique was used to assess apoptosis.

健康成年无眼部疾病青紫兰兔40只，采用计算机随机数字表法分为RD后1， 3h；1， 3， 7， 14， 28d组及正常对照组，共8组，每组5只，选取右眼为致伤眼，视网膜下注射透明质酸钠建立RD模型；分别于建立模型后按时获取兔眼标本，以TUNEL法观察视网膜神经感觉层细胞的凋亡情况。

Peribulbar injection of dexamethasone 0.5mg was applied in the experimental group every other day 48 hours after operation to the end of the experiment, and the growth state of conjunctiva in both groups were observed. 2 HA implants from experimental group and control group respectively were extracted for testing the positive expression of VEGF by immunohistochemistry 3,7,14,21,28 days after operation.

术后滴妥布霉素滴眼液4次/d，实验组术后48h起隔日球周注射地塞米松0.5mg和实验结束，并观察两组的结膜生长情况；实验组与对照组分别于术后 3，7，14，21，28d摘取2只HA植入物行免疫组织化学检测VEGF阳性表达情况。

Small Longzeyuan Changping district to be two years in the kindergarten issue has finally been resolved hope that today, Changping Commission head told reporters after the Board, property, developers, owners quadripartite discussion at the Board of Education will host three days a year without a transfer kindergarten facilities, and a week later to the tender, Please have the strength to the District Office Park kindergarten, after expert assessment and owners approved, by the end of September for the opening round.

昌平回龙观龙泽苑小区入住两年迟迟无法解决的幼儿园问题终于有了解决的希望，今天，昌平教委负责人向记者表示，经过教委、物业、开发商、业主四方讨论，教委将在三天之内接收建成一年多未移交的幼儿园设施，并在一周后向全市招标，请有实力的幼儿园入区办园，在经过专家评估和业主认可后，争取在9月底以前开园。

No matter when you start,the more importantly is that do not stop after started No matter you end ,the more importly is that do not reget after ended

不论你在什么时候开始，重要的是开始之后就不要停止。不论你在什么时候结束，重要的是结束之后就不要后悔。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

" Zhang Baoquan says,"When average person cuts an apple end up is cut, nucleus of the apple after incision is nuclear; I cut an apple to want sidewards to cut, the nucleus after opening an apple can present the appearance that gives five-pointed star, this is malic heart.

&张宝全说，&一般人切苹果时都竖着切，切开后苹果核就是核；我切苹果要横着切，打开苹果后核会呈现出五角星的形状，这就是苹果的内心。

This brought fixed cost, but it also is meant at the same time use a source to won't make you singlehanded assume a problem.

这带来了一定的成本，但它同时也意味着使用开源不会让你独力承担问题。

He gained a small fortune in real estate.

他在房地产上发了一笔小财。