查询词典 after a time

Be in after all previously very inside the time that grows a paragraph, after man-made has visited this site, searching a machine is empty-handed and return, it grew itself with respect to corresponding attune later periodic to the pay a return visit of this site, then abrupt some day has this site information was updated, search robot also cannot be aware of quickly.

毕竟在以前的很长一段的时间内，搜索机器人造访过这个站点后都是空手而归，后来它就相应的调长了自己对这个站点的回访周期，于是这个站点突然某一天有信息更新了，搜索机器人也不能快速察觉。

Brad Paisley -- Letter To Me 写给自己的信 If I could write a letter to me And send it back in time to myself at 17 First I'd prove it's me by saying look under your bed There's a Skoal can and a Playboy no one else would know you hid And then I'd say I know it's tough When you break up after seven months And yeah I know you really liked her and it just don't seem fair All I can say is pain like that is fast and it's rare And oh you got so much going for you going right But I know at 17 it's hard to see past Friday night She wasn't right for you And still you feel like there's a knife sticking out of your back And you're wondering if you'll survive You'll make it through this and you'll see You're still around to write this letter to me At the stop sign at Tomlinson and Eighth Always stop completely don't just tap your breaks And when you get a date with Bridgett make sure the tank is full On second thought forget it that one turns out kinda cool Each and every time you have a fight Just assume you're wrong and dad is right And you really should thank Mrs.

如果我能写封信，寄回给十七岁时的自己。那里有一个啤酒罐和一本"花花公子"，没人知道你把它们藏在那儿。然后我要说那很令你难受，当你交往了七个月的女孩儿与你分手，是啊我知道你好喜欢她，那似乎不很公平，但我只能说那是短暂的痛苦而且也极少发生。哦，你有着美好的未来，但我知道十七岁时很少有谁人去想周五晚上以后的事儿。她不适合你，你却依然心如刀绞，不知能否活下去。但最终你能熬过这一切而且你会看到你仍然好好的并写这封信给我。在Tomlinson与第八街的交口处，停车信号时一定要将车停住而不仅仅是点一脚刹车。当你和Bridgett约会要确信油箱是加满的，再仔细想想，别太在意了，那个女孩儿并不很酷。每一次的争执，就只当你是错了而爸爸是对的。而且你真要好好感谢Brinkman老师，她为你花费了那么多额外的时间，好像她看到了地下的钻石，她不停地打磨直到你闪闪发光。

Cheng days sales manager Chen Xinhua that as a real estate sales, the launch of "property tax" after the sale of a house price decline will, where appropriate, generally speaking will attract some of the people ahead of time to buy a house, which is a real estate sales stimulation, but the "property tax" effects of the implementation will be apparent for a long time.

上海房地产经纪有限公司销售经理程天陈新华说，作为房地产销售，推出"物业税"后出售的房屋价格下降将在适当情况下，一般来说会吸引一些人提前买房子，这是一个房地产销售刺激，但"物业税"的执行情况的影响将是明显的很长一段时间。

A morning after the National Day, mr Zhang that works in Changsha presses common habit, come to dot of booth of a breakfast, draw out two-spot money to buy 4 sesame seed cake, after faker has received fund, did not resemble giving him in time in that way clip sesame seed cake as one used to do, extend a hand to say to him again however: Full registers? horn fund -- because sesame seed cake rose in price!

2003年国庆节后的一个早晨，在长沙工作的张先生按平常的习惯，来到一个早餐摊点，掏出两元钱买4个烧饼，摊贩接过钱后没有像往常那样及时给他夹烧饼，而是又伸出手来对他说：还要4角钱——因为烧饼涨价了！

Perhaps i say like this,it's not honesty.my heart just the same feel a little lose.when all is said and done,it's a decision which is forced to make up.it's not my voluntary.it always ask us to think that we are the people to be cast away.at the same time,because of my parents,i also hope to accompany with them when they are still healthy.so i also lean to come back to china.on the other hand,i can understand the decision of the school.after all the school broke ground only a short time,it has many necessary consumption,it needs many money to supply.but we are too valuableness for the school,even if we are not satisfied with the treatment and the salary.only for the situation that school has not gived us any explanations,and then has made the decision.i think it is not done.kick down the ladder,it's not a good feeling.well,china has a old saying,"gather well,and fall apart well."

或许这样说，并不是很诚实。内心还是有些失落的，毕竟这是被迫做出的决定，还不是我主动的，总有一些被人抛弃的感觉。但是，也因为我一直考虑到父母，我希望在他们身体健康的时候，自己可以陪伴在他们身边，所以还是很倾向回国的。另一方面，我也可以理解学校的决定。毕竟学校创办之初，有许多需要花钱的地方，我们对于学校略显"昂贵"了一些，即使我们本身并不满意。只是学校在没有给我们任何说法的情况下便作出了这样的决定，还是有些失妥当的。有些过河拆桥的味道。罢了，中国有句老话，"好聚好散"。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Have according to considering to show close most probably computer a group of things with common features can arise Eye Fatigue, Eye Dry, have a headache, joint of gape, artifice, cervical the symptom that reachs shoulder ache, both hands grip appears to be worth the phenomenon of decrescent after the person that at the same time 7 is become is working, beat clavier in addition for a long time to be able to make nearly 4 become computer a group of things with common features occurrence finger ache accompanies the generation with phlogistic tendon, these are computer terminal disease group.

据研究显示有近八成的电脑族会产生眼睛疲劳、眼睛干涩、头痛、打哈欠、手腕关节、颈部及肩膀疼痛的症状，同时有七成的人在工作后出现双手握力值变小的现象，此外长期敲打键盘可使近四成的电脑族出现手指酸痛并伴随肌腱炎的产生，这些就是电脑终端机症候群。

The author comes to some conclusions as follow: The Khanate of Kipchak kept the submissive relation to the house of the grand khan and supported the supreme khan of Mongol Empire in the inner politics and the policy to the outside, which gave certain supports to the early communication in the Mongol Yuan dynasty; The Khanate of Kipchak should account for the stop of the communication caused by the foundation of the Khanate of Ogotai which had been supported by the Khanate of Kipchak during the period of the reign from Berke to Mangu Timur; The short-time stop of the communication was also Mongolian central government"s tragedy. The relationships of the Khanate of Kipchak to Yuan dynasty began to become normal during the course of the reign of Tuda Mangu ,Tula-buqa and Toqatai khan, in which the Khanate of Kipchak gave certain devotions to "peace" of the Mongol Khanates. Their relations had been best after the separation of Mongol Empire during the time of ozbeg and Janibeg"s reign which was. the rush hour of our economic and cultural intercourses with the Khanate of Kipchak and other west countries. The development of the relationships to Egypt accelerated the intercourses between this two countries, which indirectly widened the ways of the Sino-Arab communication in the economy and cultures. The relations to Ire Khanate had been very bad for a long time. This stopped the convergence of the Steppe Silk Roads and the Oasis Silk Roads. In other side, this helped the intercourses between Ire Khanate and Christian Europe.

本文认为，蒙古帝国时期，无论在内政还是外交方面，钦察汗国都维护着蒙古大汗的权威，保持着与蒙古大汗廷的臣属关系，这为蒙元早期中西经济文化交流需要的条件做出了一定贡献；别儿哥至忙哥帖木儿统治时期，钦察汗国应对因窝阔台汗国的崛起而导致的中西交通的阻断负一定责任，但这同时也是蒙古中央政府的悲哀；脱脱蒙哥至脱脱统治时期，两国关系开始逐渐恢复，钦察汗国为蒙古诸汗国的&约和&贡献了自己的力量；月即别和札尼别统治时期是两国关系的最密切时期，也是双方经济文化交流和中西交通的高峰期；钦察汗国与埃及关系的发展，一定程度地拓宽了中阿经济文化交流的渠道；钦察汗国与伊利汗国关系恶化和长期的紧张，一方面阻碍了草原丝路与绿洲丝路的交汇，另一方面促进了伊利汗国与欧洲的经济文化交流。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Sensory impairment and urinary dysfunction also had developed, and paraparesis persisted after the hypokalemia was corrected during hospitalization. An optokinetic nystagmus test showed bilateral sick waves and at the same time a cerebrospinal fluid analysis was normal. A blood examination disclosed borderline anti-nuclear antibodies and an elevated serum IgG level. Her cervical spinal cord showed mild swelling and contrast-enhancement when examined by magnetic resonance imaging. Neither a brain stem nor an optic nerve's lesion was found. Based on the above, acute transverse myelitis was diagnosed. All the symptoms present in the patient relieved gradually after 5-days of pulsed intravenous methylprednisolone therapy.

一50岁女性，因为眩晕、前额头痛、视力模糊、恶心、呕吐及两侧下肢无力而求诊，於低血钾矫正后，两侧下肢无力依然持续，并发生胸部以下感觉缺损及排尿困难等现象，视运动性眼振检查显示两侧波形障碍，脑脊液检查正常，血液学检查显示抗核酸抗体及血清IgG指数偏高，显影剂增强磁振造影显示颈段脊髓肿大且略为显影，并无脑干或视神经的病灶，诊断为急性横断性脊髓炎，给予静脉注射类固醇后症状逐渐缓解。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷