查询词典 after a manner

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

The ceo of thedhgate ,wang shutong ,is the perfect example of the ambition and action.after gratuation she worked as a teacher in qinghua university,which was envied by others.but she was not satisfied with the comfortable life.her tough will resulted in her enjoying the challenge.after two years,she resigned and left the henhouse,and manged to be employed by microsoft ,flied in the sky.she broke through all restraint and found her own area.ever since she warned herself she must be on the ground,modest and donot aim too high .six years of hard work made her the employee who promotes fastest among the colleaguesand the youngest department manger.however, it was the beginning of her dream .when everyone thought she would work continually she resigned again.which was decided after consideration.her dream was to start her own business.with the dream driving,she undertook to set up the dhgate.in the beginning the life was so difficult that many things were waiting for her,but she didnot move backand faced the difficulties.

敦煌网首席执行官王树彤就是抱负与实践完美结合的典范。毕业后留在清华任教的她让旁人羡慕之极，但自己却并不满足安逸的生活，坚韧的意志力铸就她生性喜欢挑战。两年后，她毅然辞职，离开鸡舍，过五关斩六将闯入外企微软，飞向了天空。理想与实践使她冲破世俗的禁锢，找到了自己天空。进入微软后，她告诫自己要脚踏实地，谦虚谨慎，不要好高骛远。六年的踏实苦干使她成为同期进入微软而升职最快的人，且是中国最年轻的部门经理。然而这只是王树彤梦想的开始，当所有人都认为她会继续留在公司发展的时候，她辞职了，但绝不盲目。因为她知道自己的梦想是创业。终于在梦想的驱使下，她再一次付出了果敢的行动创办了如今的敦煌网。创业初期异常艰难，有很多很多琐碎的事情要自己做而且有很多很得困难需要自己去解决，但她并没有退缩，而是迎难而上。

After turning on of the averaging mode of several measuring results or after carrying out of the series of the six simultaneous measurings the new series of measurings should be carried out only after turning the tonometer OFF and turning it ON again.

打开数次测量结果求平均值模式后或者连续测量6次后，新的连续测量应当只在关闭重新打开后进行。

Is scratched after after by the cleaning liquid microwave oven several minutes bright and clean bright new, after wipes keeps the fragrance, traces with the hand cannot feel greasy dirt,The effect is mirable!

被清洁液擦后的微波炉几分钟后光洁亮新，抹后留香，用手摸感觉不到一点油污，效果令人惊叹！

When the effect ofNTP was examined after depletion of monoamines in the spinalcord by intrathecal neurotoxins, the antiallodynic and antihyperalgesiceffects were still observed after serotonergic denervation,but not after noradrenergic denervation.

鞘内给予神经毒素耗竭脊髓单胺递质后检查 NTP 的作用时，去除5羟色胺能神经支配后 NTP 的抗触觉性痛感和抗痛觉过敏作用仍然存在，但是去除去甲肾上腺素能神经支配后则消失。

Conflicts between statements and transcriptions of this kind will be discussed further below4,the gestures for consecutive stops overlap,so that stops are unexploded when they occur before another stop in words 5,in many accents of English,syllable final/p,t/are accompanied by an overlapping glottal stop gesture ,as in pronunciations of tip,(this is another case where transcription cannot fully describe what is going on)6,in many accents of english ,/t/is replaced by a glottal stop when it occurs before an alveolar nasal in the same word7,nasals are syllabic at end of a word when immediately after an obstruentNote that we cannot say that nasals become syllabic whenever they occur at the end of a word and after a consonant.the nasals in kils,film are not syllabicity of /l/by saying simply.8,the lateral /l/ is syllabic at the end of aword when immediately after a consonant.

之间的冲突的声明和抄录这种将进一步讨论见下文） 4 ，连续的姿态站重叠，因此，车站未发生时之前停止在另一个字 5 ，在许多口音的英语，音节最后/磷，吨/年的同时，重叠喉塞音的姿态，在发音的提示，（这是另一个情况下，转录不能完全说明是怎么回事） 6 ，在许多口音的英语，/吨/年是取而代之的是一个喉塞音发生时肺泡鼻前的同一个词 7日，鼻骨的音节在一个单词的结尾时后立即obstruent 请注意，我们不能说鼻骨成为音节何时何地发生在一个单词的结尾之后consonant.the鼻骨在kils ，电影不是syllabicity的/升/说简单。 8 ，横向/升/是音节在年底时aword后立即辅音。

After the drum on the treatment is the key to a new after the paper in a zhangba unwatering of copy paper, print this screen you will pass a carbon into drum in a white, then remove the copy paper, so that the screen is clearly see what parts should be single pad and which parts do not pad, and which part should be dredged, dredged area must have a slope should be completed on the knife, which lost network and wrapped a drum after proofing effect, when you are satisfied you can open mechanism cards and membership cards.

版上好后滚筒的办理不离是盖脚换上陈的滚筒纸后本质不收一张八开的复写纸，不收空印一次这样网版不离会堵住复写纸印到滚筒边的白纸上，然后把复写纸掏出，这样网版不离澄辉不天看出哪些部位不该独垫，哪些部位不垫，哪些部位不该挖去，挖去的部位必定用不该小刀修成有坡度，这样不会失网点，包好滚筒后打样一张看成果，如果不满即可开机制卡和会员卡制作。

Under the microenvironments of fruit bagging,Alternaria had always been the major fungi in different bags,sites of fruit and weather conditions during the whole growing season. As the secondary major fungi,Penicillium had always been after July and emerged later in inner bag and cortical pore than other sites.In the bag with which the inner bag was red and waxing,Trichoderma was isolated first in pericarp without cortical pore and inner bag as well as later in cortical pore after August.In \'Kobayashi\' bag which inner bag was red and waxing, Aspergillus and Fusarium were isolated in pericarp without cortical pore and cortical pore after bag removed.Alternaria and Penicillium were isolated in unbagging fruit and \'Qianwei\' bag which inner bag was red and unwaxing and the date Penicillium was isolated in CK was 45d later than bagging treatment.Under high temperature weather condition,there were no differences in the fungi population structure on fruit surface among all treatments.And the fungi population structure on fruit surface in bagging apple was more than that of unbagging ones under overcast and rainy condition.

在苹果套袋微域环境下，不同育果袋、果实不同部位、不同时期、不同气象条件下的真菌主要是链格孢霉，且在套袋后的整个生长季均可分离到；青霉为第二大真菌，在7月份后均可分离到，在非皮孔部位的出现要早于内果袋和皮孔部位；8月份后在内袋红色涂蜡的育果袋上分离出了木霉，其先出现在非皮孔处和内果袋，然后出现在皮孔处；曲霉和镰刀菌仅在内袋红色涂蜡的&小林&袋的非皮孔部位和皮孔部位于摘袋后分离出；在内袋为黑色且未涂蜡的育果袋和未套袋果上仅仅分离出链格孢霉和青霉，且青霉分离出的时期要比套袋果晚45d。

Said the so-called "reverse-arbitrage" refers to: the New Deal as a result of the valuation of the net after the implementation of the Fund have been adjusted, and when the bear market in stocks resume trading after suspension consecutive limit of the share price lower than the index of income method of valuation estimated price trends, in a public notice prior to conversion, the base of the public can redeem the fund after the conversion to avoid a further decline in net losses.

说，所谓的&反向套利&是指：新政作为净价值后，基金的执行情况进行了调整，结果，当熊市在股票停牌后恢复的股价比收入较低的估价方法指数连续限制交易价格趋势，估计在公告中，转换之前，市民的基地可以在转换后赎回基金，以避免进一步下降净损失。

After the novels, after the teacups, after the skirts that trail along the floor

在读小说以后，在饮茶以后，在长裙拖过地板以后

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。