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The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

NO concentration did not change significantly in the groups with M or S-PH and mild PH before and after CPB.NO level in the group with non-PH 30 min after CPB decreased significantly as compared with that after induction of anes thesia.

伴肺高压两组病人CPB前后NO浓度无明显变化，no-PH组CPB后30分钟时较麻醉诱导后明显降低(P＜0.05)。

The index case was a 5-month-old infant girl who had anisocoria from 4 weeks of age. One hour after 1% apraclonidine hydrochloride was instilled in each eye, there was blanching of the periocular skin, and the parents reported that the infant was excessively drowsy. Two hours after receiving apraclonidine, the infant was taken to the emergency department for lethargy and unresponsiveness, with bradycardia (84 beats/min), shallow respiration (40 breaths/min), and blood pressure 115/85 mm Hg. Oxygen saturation was 80% but improved rapidly to 100% when oxygen was given via a face mask. Eight hours after receiving apraclonidine, the infant had returned to normal.

指标案例是一名5个月大的女婴，她从4周大时就有瞳孔大小不等的状况；在1%的apraclonidine氯化氢徐徐滴点她的双眼后1个小时，眼眶周边的皮肤即变白，而她的家长表示她变得非常嗜睡；在使用apraclonidine 2个小时之后，该名女婴因嗜睡和无应答性，以及心搏过慢（每分钟84下）、呼吸浅弱（每分钟40次）以及血压115/85 mm Hg等症候而被送进急诊室；血氧饱和度原先为80%，在面罩给氧后迅速回复到100%，在使用apraclonidine 8个小时后，该名女婴终于回复健康状态。

The index case was a 5-month-old infant girl who had anisocoria from 4 weeks of age. One hour after 1% apraclonidine hydrochloride was instilled in each eye, there was blanching of the periocular skin, and the parents reported that the infant was excessively drowsy. Two hours after receiving apraclonidine, the infant was taken to the emergency department for lethargy and unresponsiveness, with bradycardia (84 beats/min), shallow respiration (40 breaths/min), and blood pressure 115/85 mm Hg. Oxygen saturation was 80% but improved rapidly to 100% when oxygen was given via a face mask. Eight hours after receiving apraclonidine, the infant had returned to normal.

指标案例是一名5个月大的女婴，她从4周大时就有瞳孔大小不等的状况；在1%的apraclonidine氯化氢徐徐滴点她的双眼后1个小时，眼眶周边的皮肤即变白，而她的家长表示她变得非常嗜睡；在使用apraclonidine 2个小时之后，该名女婴因嗜睡和无应答性，以及心搏过慢（每分钟84下）、呼吸浅弱（每分钟40次）以及血压115/85 mm Hg等症候而被送进急诊室；血氧饱和度原先为80%，在面罩给氧后迅速回复到100%，在使用apraclonidine 8个小时后，该名女婴终於回复健康状态。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

Results Balanus visualization and penis retraction were showed 3 months after three-point fixation of the penis in 64 cases; penis came to visualization and became 2~3 cm longer after modified Devine's technique in 68 cases; while penis came to visualization and became 3~4 cm longer with good correction after modified Foreskin-Deglove and Shaft-Fix procedure in 80 cases.

结果三点式阴茎固定术64例术后3个月龟头显露，阴茎回缩，外观短小。改良的Devine术68例，术后阴茎显著外露，阴茎较术前延长2~3cm。

Animals were randomly divided into four group: group 1, as control (n=8), without induction of epilepsy; group 2 (n=8), 30 min after intrahippocampal injection of benzylpenicillin; group 3 (n=8), 60min after intrahippocampal injection of benzylpenicillin; group 4 (n=10), 60 min after intrahippocampal injection of benzylpenicillin with EA treatment in the last 30 min (P60 +EA); At the end of each treatment, EEG of each animal was recorded and its RP was analyzed.

我室以往工作表明，针刺具有抗痫作用，而MT也具有抗痫作用，且有资料表明，癫痫患者内源性MT水平发生变化，因此，我们又运用毛细管电泳技术结合电化学检测法，对青霉素所致痫样发作的大鼠在癫痫发作期间和电针抗痫时松果体、海马和血浆内的MT水平进行了测定。

The four protozoa had different changing patterns in rhizosphere and nonrhizosphere soil. About 90 times more Colpoda were determined in rhizosphere than in nonrhizospher at 52 days after sowing. There were more Anweba in rhizosphere than in nonrhizosphere at 74 days after sowing. Much more Bodo were found in rhizosphere at 32 days alter corn sowing. However, the number of the Bodo in rhizosphere soil was only 10% of that in the nonrhizosphere soil at 52 days after sowing. Rhizosphere always contains more Cercomonas than nonrhizosphere within the whole growing season of corn.

不同的原生动物的根际效应差异很大，肾形虫在抽雄期R/S比接近90；变形虫在吐丝时期表现出一定的根际效应；波豆虫在大喇叭口时期表现出比较强烈的根际效应，但在抽雄期则表现出十分强烈的负根际效应，根际土壤中波豆虫数量不到非根际土壤的10%；尾滴虫在玉米整个生育时期都表现出微弱的根际效应。

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性，经过滤和离心浓缩的病毒上清液60%细胞表达P-GP，是病毒原液基因转移率的2倍；RT-PCR表明基因转移后，肿瘤组织内mdr1 mRNA的表达量明显增加。

Hippocrates after curing many diseases himself fell sick and died. The Chaldaei foretold the deaths of many, and then fate caught them too. Alexander, and Pompeius, and Caius Caesar, after so often completely destroying whole cities, and in battle cutting to pieces many ten thousands of cavalry and infantry, themselves too at last departed from life. Heraclitus, after so many speculations on the conflagration of the universe, was filled with water internally and died smeared all over with mud. And lice destroyed Democritus; and other lice killed Socrates.

希波克拉底一生医人无数，最终自己也得病死去；Chaldaei家族预言了很多人的死亡，这一命运最终也降临到他们头上；亚历山大，Pompeius和尤里斯凯撒一生毁城极多，在战斗中杀死骑兵步兵无数，最终他们也难逃一死；赫拉克利特对宇宙间的灾难作了如此多的思考，但他溺水而亡，死时全身满是淤泥；德谟克利特承受小人中伤；苏格拉底则被小人所杀。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。