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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

To professional identity of kindergarten teacher majors, there exist such differences as: nature of kindergarten, age of the kindergarten teachers, years of serving as a teacher, the educational background, professional post and salary and so on, which can be seen in the following aspects: 1 the degree of professional identity of kindergarten teachers in non-governmental kindergarten is higher than that of teachers in governmental kindergartens; 2 the degree of professional identity of kindergarten teachers aged between 21 to 25 is the highest, while those aged between 31-40 is lowest; 3 the professional identity of kindergarten teachers who have taught for 2 to 14 years in school is of the highest, while those for 15 to 25 is of the lowest; 4 those whose academic background is intermediate rank are considered to be the highest in the professional identity while those graduated from the junior middle school are regarded to be the lowest, 5those whose professional post ranks second in primary school are the highest level in professional identity, while those whose professional post are of first rank are in the lowest; 6those whose income is between 501 and 1000 RMB are regarded to be on the top in their professional identity while those whose income above 1500 RMB are in the lowest level.

主要表现为：1民办幼儿园教师职业认同高于公办幼儿园教师；2年龄在21—25岁之间的教师职业认同最高，31—40岁之间的教师最低；3教龄在2—14年间的教师职业认同最高，15—25年间的教师最低；4学历为中职的教师职业认同最高，初中学历教师职业认同最低；5职称为小教二级的教师职业认同最高，小教高级的教师最低；6工资收入在501—1000元的教师职业认同最高，1500元以上的教师最低。3、在其他因素上，如"是否担任行政职务"、"婚否"、"所带班级"、"班额大小"等方面，幼儿教师的职业认同没有显著差异。

Out where the river broke The bloodwood and the desert oak Holden wrecks and boiling diesels Steam in forty five degrees The time has come To say fair's fair To pay the rent To pay our share The time has come A fact's a fact It belongs to them Let's give it back How can we dance when our earth is turning How do we sleep while our beds are burning How can we dance when our earth is turning How do we sleep while our beds are burning The time has come To say fair's fair To pay the rent, now To pay our share Four wheels scare the cockatoos From Kintore East to Yuendemu The western desert lives and breathes In forty five degrees The time has come To say fair's fair To pay the rent To pay our share The time has come A fact's a fact It belongs to them Let's give it back How can we dance when our earth is turning How do we sleep while our beds are burning How can we dance when our earth is turning How do we sleep while our beds are burning The time has come To say fair's fair To pay the rent, now To pay our share The time has come A fact's a fact It belongs to them Let's give it back How can we dance when our earth is turning How do we sleep while our beds are burning

在哪里爆发的河流该bloodwood和沙漠橡树霍顿沉船和沸腾柴油蒸汽在45度现在时机已到如果说公平公正付房租我们的支付份额现在时机已到一个事实是事实它属于他们让我们回到我们怎么能跳舞时，我们的地球是转折点我们怎么睡觉，而我们的床燃烧我们怎么能跳舞时，我们的地球是转折点我们怎么睡觉，而我们的床燃烧现在时机已到如果说公平公正付房租，现在我们的支付份额四个轮子吓唬cockatoos 从金托尔东Yuendemu 西部沙漠的生命和呼吸在45度现在时机已到如果说公平公正付房租我们的支付份额现在时机已到一个事实是事实它属于他们让我们回到我们怎么能跳舞时，我们的地球是转折点我们怎么睡觉，而我们的床燃烧我们怎么能跳舞时，我们的地球是转折点我们怎么睡觉，而我们的床燃烧现在时机已到如果说公平公正付房租，现在我们的支付份额现在时机已到一个事实是事实它属于他们让我们回到我们怎么能跳舞时，我们的地球是转折点我们怎么睡觉，而我们的床燃烧

When motion is towards the location of the addressee,come is much preferable to go in English while both laiand quare common in use in Chinese.When motion is towards the location of the addressee at a past time,laimust be used in Chinese if the addressee is there at the time of utterance while qumust be used if the addressee is not there at the time of utterance.By contrast,come is used in English either when the addressee is there or when he is not there at the speaking time,while go is only used when the addressee is not there at the time of utterance. When both the speaker and the addressee serve as the figure,which is indicated by the phrases like with me/us/you in English and the phrases like he ni,he nimen,gen wo,gen womenin Chinese,come is much preferred to go for the figure"s motion,while in the same context,quis used for the figure"s motion.What"s more,come is more frequently used to indicate the figure"s movement towards a place where neither the speaker nor the addressee is located at the time of utterance,while quis often used in Chinese in the same case.

当移动体朝向听话人所在方位移动时，英语多用come，汉语可用"来/去"；当移动体在一个过去的时间朝向听话人所在方位移动时，汉语可用"来"表示听话人在说话时间仍在位移参照点，用"去"表示听话人在说话时间已不在位移参照点，而英语在两种情况下都可用come，用go则表示听话人在说话时间已不在位移参照点；当说话人和听话人一起作为移动体而句中主语只是说话人或听话人一方时，英语多用come，汉语多用"去"。

I ran in the games in all positions: While I cheered for athletes with all the audiences, while I ran to the seashore to help to pick the ball, while I helped the scorekeeper scored, while I helped the referee to check the judgments, while I ran to harmonize disputes etc.

我奔跑于在赛场上的各个岗位：一会儿我帮着观众一起呐喊，一会儿我跑到海边帮忙捡球，一会儿我帮记分员记分，一会儿我帮裁判员看球，一会儿前往协调小争议……。

On the basis of historical investigation,theauthor sums up five-point revelations:The consolidation of the Party'sruling foundation from the base while not turning things inside out;theconsolidation of the Party's ruling foundation in full-scale while not beingnarrow and unilateral;the consolidation of the Party's ruling foundationstep by step while not being desperate for success;the consolidation of theParty's ruling foundation concretely and historically while not beingdeadlock and conservative;the consolidation of the Party's rulingfoundation with open mind while not being self-closed.

在此基础上，总结了巩固党的执政基础历史实践的五点主要启示，即&从根本上巩固党的执政基础，决不能本末倒置&，&全面地巩固党的执政基础，决不能狭隘片面&，&循序渐进地巩固党的执政基础，决不能急于求成&，&具体地历史地巩固党的执政基础，决不能僵化保守&，&以开放的眼光巩固党的执政基础，决不能自我封闭&。

My house to do the last baby, I remember when pregnant pregnant, listen to experts said doing so would close much of the most effective way is to give her juice, cucumber juice to eat, my baby after four months since a food supplement is the case, then I am on the day I made him eat the apple and banana juice into the mud a little pressed for her to eat every day insist that the increase in the volume a little bit, and now my son 10 months, and no longer the case, each tried all the normal, if you are so artificial feeding, as well as give attention to your baby to drink water, I will give my baby milk food partner, which is the lotus leaf, linesinine what the powder mill, as a little bitter, so I home to eat a little baby bottle of glucose in to soak in his tea to drink now that we are still very good.

我家宝宝过去也这样，记得怀怀孕的时候，听专家讲做的时候说便密厉害的话最有效的方法就是榨黄瓜汁给她吃，我家宝宝自从四个月后加了辅食也是这样，后来我就每天给他吃我自制的苹果和香蕉各一点榨成果汁泥让她吃，每天坚持，一点点的加量，现在我儿子十七个月了，再也没有这样了，每次的便便都很正常，如果你是人工喂的话，还有注意都给宝宝喝水，我给我家宝宝吃的奶伴侣，里面是荷叶，莲心什么磨的粉粒，因为有点苦，所以我就家一点宝宝吃的葡萄糖泡在奶瓶里给他当茶喝，到现在我们还在喝，很不错了。

After introducing the little sheep chain development present situation and development trend of the industry, this paper analyzes the little sheep facing opportunities and threats, around the little sheep interlinks orgnaization brand management, puts forward some little sheep chain management ability raise proposals.

在介绍了小肥羊连锁机构的发展现状及行业发展趋势的基础上，分析了小肥羊所面临的机会和威胁，围绕小肥羊连锁机构品牌经营方面存在的问题，提出了增强小肥羊连锁机构提高经营管理能力的建议。

At first hears Great Master father to calling ' water ' a lot of, hear the voice that drinks boiling water, next the madam begins to call ' duck ', ' duck ', the ' chicken ' that crosses a madam a little while to say a master worker again fat big, after eating chicken, great Master father calls ' to burn ' of leg of pork all the time, looking is pork leg taking a pig, passed a little while next, great Master father is shouting to want to break up again, be this to eating ' fish ' apparently, why should break up otherwise?

起先听到大师父叫着'水'好多，就听到喝汤的声音，然后夫人开始叫'鸭'、'鸭'，过一会夫人又说师父的'鸡'又肥又大，吃完鸡后，大师父一直叫'烧蹄子'，看来是吃猪蹄膀了，然后过了一会，大师父又嚷着要翻过来，这回显然是在吃'鱼'了，不然为什么要翻过来？

Since the above six are self-sufficient man likes to do the job, and the pursuit of self-worth, feel self-care, personal space, respect for another's privacy, pay attention to personal style and taste, do not follow the crowd, there is awareness of the law of "80 After "will consciously dislike these acts: for example, talk to others for no reason," 80 after the "more agreeable to the West are more concerned about the self-opinion, very few non-Road, concerned about others; such as, enjoy preaching others," 80后" from an early age from schools, parents, etc. too much preaching, preaching and therefore acts very much, resulting in antagonistic, their attention; For instance, to belittle the advantages of others,"80 after" consider embodies self-worth is not to derogate others premise, the more The more derogatory of others to explain their value enough, are their attractions; For instance, smoking in the office,"after 80" would be a focus on self and respect for others the organic integration of generation, because these two are not always contradictory, if that "after 80" only know that take care of their own convenience, on the wrong; such as, the mentality of not enough sunlight,"80后" feel self-care, therefore, delighted to do anything on an individual basis or as a premise, we think that good , but their psychological upset, does not endorse, they will not act in accordance with the meaning of the U.S., the ultimate manifestation of personal values on the individual feel, are their characteristics; For instance, loud make personal phone calls,"80, after" personal space, respect for others Privacy, therefore, in a public office area will be less and less to see the "80 after" unscrupulous people talking about the workplace their own affairs, the concept of privacy, especially popular in this generation will be reflected in the workplace in the past.

因为，上述6条都是不够自我的职场中人喜欢做的，而追求自我价值、在乎自我感受、重视个人空间、尊重他人隐私、讲究个人风格与品味、不随波逐流、有法律意识的"80后"会自觉地不喜欢这些行为：比如，无端议论他人，"80后"比较认同西方人较为关注自我的观点，很少道人是非，关注别人的事；比如，喜欢说教别人，"80后"从小受到来自学校、家长等的太多说教，因此对说教行为很反感，从而产生逆反，自己注意；比如，贬低别人优势，"80后"认为体现自我价值不是以贬损别人为前提的，越贬损别人越说明自己的价值不够，是他们的过人之处；比如，在办公区吸烟，"80后"将是注重自我和尊重别人有机结合的一代，因为这两者始终不矛盾，如果认为"80后"只知道照顾自己的方便，就大错特错了；比如，心态不够阳光，"80后"在乎自我感受，因此，任何事情是以个人高兴与否作为前提的，大家觉得好，但自己心理不高兴、不认可，便不会根据大家的意思行事，个人价值最终体现在个人感受上，是他们的特点；比如，大声打私人电话，"80后"重视个人空间、尊重他人隐私，因此，在公共办公区将越来越少地看到"80后"职场人肆无忌惮地谈论自己的事情，隐私的概念在这一代特别流行，将会体现到职场中来。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。