查询词典 affinity

In the absence of coorperative effect, the reaction between lectin molecules and their receptors on cell surface can be described as below when equilibrium is reached:In which LT denotes total lectin concentration, LF free lectin concentration, mva-lency of lectin, ni number of receptors with affinity constant Ki to lectin, CT total lectin concentration.

本文提出一个描述细胞与多价凝集素反应的平衡关系式和用紫外分光光度法测定游离凝集素浓度求受体数量及其与凝集素的亲合常数的方便方法。

Keywords porphyrin dimer;DNA;apparent affinity binding constant;photocleavage

双卟啉； DNA；表观键合常数；光切割

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

The Scatchard analysis of data revealed a linear relationship between the amount of specific binding and the ratio of specific binding to free 125 I (D Lys 6)LHRH, indicating a single class of high affinity sites. Equilibrium dissociation constant was 0 34 nM in pituitary membrane preparation and 0 43nM in isolated pituitary cells.

特异性结合随125 I( DLys6 ) LHRH 量的增加而增加，实验结果经Scatchard 分析揭示出特异性结合与结合和游离125I( DLys6)LHRH 之比呈线性关系，预示绍鸭垂体对( DLys6)LHRH 存在同一类高亲和力的结合位点。

The affinity constant of the anti-SQ polyclonal antibody 1405~# was 1.22×10~8L/mol. The cross reactivity with other SAs was less than 0.17%. Three hybridoms cell lines secreting monoclonal antibody against SQ were obtained.

同时，以SQ-BSA为免疫原免疫Balb／c小鼠，经融合实验获得了三株分泌抗磺胺喹噁啉单克隆抗体的杂交瘤细胞株SQl_(12)A_(10)，SQ_(26)B_7，SQ_(28)H_7。

The chemical kinetics calculations in this thesis are assessed in the time slot of 10 minutes, and we can obtain the association constant, dissociation constant and affinity constant are 2.925×106M-1s-1, 7.643×10-3s-1 and 3.83×108M-1 M-1 for IgG–Anti rabbit IgG respectively.

於化学动力学参数计算上，我们评估10分钟内的即时萤光讯号反应强度，初步推估IgG–Anti rabbit IgG之结合常数、解离常数以及亲合力常数分别为2.925×106M-1s-1、7.643×10-3s-1、3.83×108M-1，，此结果与目前商用检测产品之典型值范围相符。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

And 0.949〓 respectively. From the model, the substitution of Pro (119)αto Ala in bar-headed goose Hbα〓β〓 interface, which is unique among bird and mammal Hbs and thought to be critical for the enhanced oxygen affinity in bar-headed goose Hb, causes no significant change in protein structure.

大量的研究工作认为，鸟类和哺乳动物Hb分子中a〓β〓亚基交界面唯一的氨基酸突变：Pro（119）α→Ala对于斑头雁Hb分子O〓亲和性的提高是关键的。

Crystal structures of bar-headed goose aquomet hemoglobin and greylag goose oxy Hb were determined and compared, which confirms the previous postulation that α119 mutation leads to the higher oxygen affinity of bar-headed goose Hb, but indicates that β125 mutation might also make part contribution.

通过对斑头雁水合高铁血红蛋白和灰雁氧合血红蛋白的晶体结构研究，证实了前人关于α119突变导致斑头雁血红蛋白的氧亲和性提高的假说，同时推测β125突变可能也有部分贡献。

However, relatively lower column utilization limits its application to the complex proteome isolation. In case of binding biotin molecules to a specific kind of functional groups existed in protein structure, and flowing the digested peptides over the affinity column in which avidin has been immobilized, biotinylated peptides can be selectively separated and the complicated system is simplified.

因此根据需要，变换生物素试剂与蛋白质反应的作用位点，就可以选择性地分离出不同的目标肽段，从而大大提高亲和色谱柱的使用效率，降低亲和分离的成本；结合乙酰化同位素标记技术，还可以建立多种针对不同目标肽段的特色定量分析方法，ICAT即是将同位素标记与蛋白质巯基生物素化亲和分离成功结合的一个范例。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。