查询词典 affinity

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

The following results have been obtained: 1 Expression of SbNHX1 in E. coli enhanced Na+ and K+ tolerances, but not to others; 2 The sequence from 1353-1514bp in SbNHX1 gene played a role for SbNHX1 to Na+ and K+ tolerances; 3 The Na+/H+ antiporter of Salicornia Bigelovii Torr. catalysed Na+/H+ or K+/H+ exchange with equal affinity, which indicates that the SbNHX1 is more likely to locate on member of vacuolar, and responds to transport Na+ into the vacuole from cytoplasm.

证明了SbNHX1能抵抗Na+和K+的胁迫，但对其它盐离子没有抗性；2）证明了北美海蓬子Na+/H+逆向转运蛋白基因（SbNHX1）C末端从基因的C末端1353bp至1514bp的序列在耐盐中起一定的作用；3）证明了北美海蓬子Na+/H+逆向转运蛋白（SbNHX1）对Na+和K+交换的等亲和性，对于这一点，说明北美海蓬子Na+/H+逆向转运蛋白与定位于液泡膜上的Na+/H+逆向转运蛋白的功能相似。

These binding sites had saturability,high affinity and low capacity.

这些位点与GC的结合具有饱和性、高亲和力及低容量。

ER with low capacity,high affinity and saturable was detected in cytosol oftwenty four of 62 samples of HCC(38.7%).

62例肝癌标本均采自手术治疗的肝癌病人，雌激素受体的检测用葡聚糖包裹活性炭吸附法。

The study suggested that the optimal method not only enhanced the affinity and the maximum reaction velocity of α-amylase with its substrate, but also improved the accuracy and the sensitivity on the activity of α-amylase from M. separata larvae in assays, whereas it was not the optimal method for determining the activity of α-amylase from T. molitor, C. pipiens pollens and M. domestica larvae.

结果说明，在该优化体系下，粘虫α-淀粉酶与底物的亲和力增强，最大反应速度增大，测定酶活性的准确性和灵敏度显著提高；同时该优化体系也可作为测定德国小蠊α-淀粉酶活性的优化方法，但不适合作为黄粉虫、淡色库蚊和家蝇α-淀粉酶的最优化测定方法。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

These subvolcanics, in which basalt, basaltic andesite and andesite are dominant, are petrochemically of calc alkaline affinity of subalkaline series and sodic type, and tend to have moderate low total REE, being LREE enriched.

岩石化学上属亚碱性系列、钙碱性火山岩系、钠质类型；稀土总量中低，属轻稀土富集型，稀土分布模式为右倾平滑曲线或略有正、负铕异常；过渡族元素球粒陨石标准化曲线形态呈&W&型，锶初始值为 0 。70 5398，反映物质来源较深。

Deep professional knowledge , intact knowledge structure, abundant practical experience, optimistic and optimistic personality , superstrong group cooperation spirit and affinity, will help me to finish every task smoothly in the twists definitely.

深厚的专业知识，完整的知识结构，丰富的实践经验，乐观豁达的性格，超强的团体协作精神和亲和力，定会助我在曲折中顺利完成各项工作任务。

In spite of the low binding affinity between the Mis13/Mis14 dimer and the Mis12/PMF1 dimer,the four proteins form a heterotetramer with a stoichiometry of 1:1:1:1 when these four proteins expressed synchronously,implying that the four subunits can act synergically to form an intact Mis12 complex in vivo.

Mis12复合物在动点上的功能已经较为清楚，但是它们分子间结合方式仍是未知的，为了解析Mis12复合物的分子构架，我们引入了共表达系统在体外表达和重组该复合物。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。