查询词典 affinity

Label-free and cleavable isotope-coded affinity tag quantitative proteomic approaches were used to quantify the differential expression of secretory proteins from 3T3-L1 adipocytes with or without insulin stimulation.

首先，采用液相色谱-质谱联用方法对胰岛素刺激与否的3T3-L1脂肪细胞的分泌蛋白进行了大规模的定量分析；胰岛素刺激后，242种分泌蛋白表现为显著变化，其中55种蛋白质下调，187种上调。

Methods SMMC-7721 cell apoptosis was induced by HCPT and the mitochondria were isolated with a mitochondria isolation kit. Mitochondrial proteins labeled with a cleavable isotope-coded affinity tag were identified and quantified using two-dimensional liquid chromatography/tandem mass spectrometry.

用羟基喜树碱诱导肝癌细胞SMMC-7721凋亡，提取线粒体并鉴定其纯度；用可裂解的同位素标记的亲和标签技术标记线粒体蛋白质，利用二维液相色谱-串联质谱分析鉴定蛋白质。

At the same time, we introduced negative selection, the affinity mature and clonal selection.

同时介绍了负选择和克隆选择算法。

Also lose by force with the age, constitution as to coital duration, posture of sexual life experience, sexual intercourse, penis twitchs the sexual reaction of extent, frequency and female has affinity.

至于性交持续时间也与年龄、体质强弱、性生活经验、性交体位、阴茎抽动幅度、频率以及女性的性反应有密切关系。

Objective To evaluate the cytotoxicity and cell affinity of a porcine collagenic dermal matrix to guide the improvement of the porcine dermal scaffold.

目的 通过对一种新型的猪胶原基真皮支架的细胞毒性和与成纤维细胞的亲和性进行评价，为这种新型生物材料的安全应用及工艺改良提供依据。

Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.

用免疫亲和层析法分离纯化人肾ADA，测氨法测定 ADA活性，经SDS-PAGE测定其分子量。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The article put forward the affinity between court that has compellent power and international commercial arbitration directly because arbitration that can settle dispute by non-suit is playing important role.

鉴于仲裁这一非诉讼解决争议的方式在国际商事活动中扮演着日益重要的角色已成为众所周知的事实，论文开门见山即提出法院这一具强制性的国家机关与国际商事仲裁密切且特殊的关系。

The crux and cruces deserts attention in the present creation of short telefilm,such as ,the choice of the visual angle , director assurance to the scene, interview , montage , type and artistic conception, drama conflict, the styles of the course , affinity , sound , short telefilm manner, merger of short telefilm and column, etc.

视角的选择、编导对现场的把握、采访、蒙太奇、典型与意境、戏剧冲突、过程、亲和力、声音、短片的风格、短片与栏目的整合等都是当前短片创作中的难点和关键点。由于短片的创作周期短，加上栏目化生产的一些限制，这些因素经常被编导们忽视，从而导致了短片的艺术性和欣赏性的降低。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。