查询词典 affinity

OBJECTIVE:To obtain high purified and cheap human copper/zine superoxide dismutase with genetic engineering and affinity chromatography.

目的：用基因工程的方法获得人铜锌超氧化物歧化酶的高产菌，通过发酵培养，最后纯化获得大量廉价的高纯度的hCu，Zn－SOD。

Following purification by Ni affinity chromatography and stepwise renaturation by dialysis, rB7-B5 showed sound antimicrobial activity in porcine actinobacillus pleuropneumoniae and antibiotics-resistant E. coli. The present study provides basis for the research and development of novel antibacterial preparations.

以经Ni亲和层析柱纯化和多步透析法复性的rB7-B5，对猪胸膜肺炎放线杆菌和耐药性大肠杆菌具有很好的抑菌活性，本研究为新型抗菌制剂的研制和开发奠定了基础。

Surfactant is a molecular "" amphophilic, by a non-polar oil hydrocarbon chains and polarity of affinity group together form.

暗地活性剂是一种&双干&不合子，由否极性的干油的碳氢链局部和极性的干火的基团共同组成。

And on the basis of these work, anti-bFGF Fab phage library was constructed with PMBC of autoimmune patients, such neuropathic amyotrophia patient, and two clones with very high affinity were obtained. There were 15 papers published, one paper had been incepted.

在前期研究基础上，利用神经性肌萎缩等患者血清中含有高滴度抗bFGF抗体特点，构建人源bFGF Fab噬菌体抗体库，获得2株具有较高亲和力

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质，在低温和微酸性条件下，将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱，然后再通过增加NaCl浓度的方法将该酶洗脱，从而达到其高效而经济分离纯化的目的。

In addition, the features of elemental geochemistry of all samples suggest that (1) the EarlyTriassic andesitic rocks, characterized by characteristic natures of island arc volcanics, likely erupted in the subduction setting; that (2) the generation of LateTriassic rhyolites with an affinity of Alike granite and synchronously main body granites in the Lincang batholith displaying similar geochemical features, is closely related with the postcollision or latecollision setting; that (3) although the LateTriassic basic rocks partly show island arc volcanics such as highAl contents and NbTa depletion, some of them possessing highMg contents and mostly having traceelement ratios similar to intraplate basalt, which indicates that they erupted in the postcollision rift environment.

元素地球化学特征表明，早三叠世火山岩具有典型的岛弧火山岩的特征，暗示其很可能形成于俯冲的构造环境；中三叠世晚期的流纹岩与临沧花岗岩基主体具有非常相似的地球化学特征，结合其流纹岩具有A型花岗岩属性，表明该区中三叠世火成岩形成于碰撞后的构造背景；而晚三叠世基性火山岩虽然在地球化学特征上显示出与岛弧火山岩相似的高铝和NbTa的亏损，但其中部分火山岩具有高镁和板内玄武岩的微量元素比值特征，说明这些火山岩可能形成于碰撞后裂谷环境。

Based on the study of 120 cultivated herbaceous peonies and 8 wild species native to China, the relationship among them was firstly investigated, and the result indicated that 4 narrowly-distributed wild species involving Paeonia sinjiangensis K. Y. Pan, P. anomala L., P. emodi Wallich ex Royle and P. sterniana Fletcher have no chance to be the ancestor of Chinese cultivated herbaceous peonies. Then the difference and similarity existed in the root, leaves and flowers of the wild species and cultivars was exhaustively compared and it is concluded by the equivalent affinity between P. lactiflora Pall. and the cultivars.

以原产我国的120个栽培品种和8个野生种为主要研究对象，首先根据地理分布的差异对栽培芍药与各个野生种的亲疏关系进行了分析探讨，表明新疆芍药、块根芍药、多花芍药和白花芍药等4个窄布种不可能参与我国芍药品种的栽培起源；然后对栽培品种和各野生种之间的根、叶及花等性状的差异进行了细致的比较分析，进一步证明川赤芍亦不可能参与我国栽培芍药品种的起源，草芍药和美丽芍药等2个种对我国栽培芍药的起源影响甚微。

Trilobite evidence indicates that all the plates and most of the terranes in China exhibit a close biogeographic link and may have formed part of the eastern Peri-Gondwana during the Ordovician, except for the Altay Terrane of the Northern Xinjiang Autonomous Region and the Ergen-Hinggan Terrane of the Hinggan Region, where trilobite faunas show a strong affinity with those of Siberia and Laurentia.

三叶虫证据表明，除北疆区的阿尔泰地体和兴安区的额尔古纳－兴安地体与西伯利亚和劳伦陆块在动物群方面紧密关联外，中国奥陶纪的所有板块和大部分地体均系当时东泛冈瓦纳的重要组成部分，在生物地理上彼此密切相关。

After nucleosides were extracted from urine on phenyl boronic acid affinity chromatography, the analysis was performed on a column (46 mm id×250 mm, 5 μm) at 22 ℃ using a linear gradient elution comprising 25 mmol/L KH2PO4 solution (pH 455) and 60% methanol in water with UV detection at 260 nm.

用该法测定了41例肠癌患者和52例正常人尿中15种核苷的含量(用核苷与肌酐的摩尔比表示，下同)，结果表明肠癌患者中有12种核苷的含量比正常人显著性增高(P 001)。以15种核苷的含量作为参量，结合主成分分析区分正常人和肠癌患者，对癌症病人的识别率达76%(31/41)。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。