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The macrocyclic polyamines are a kind of excellent ligands and have a substantially high binding affinity with transition metal ions. Macrocyclic polyamine metal complexes as enzyme models are widely used in chemical biology, medicine and gene therapy. 1, 4, 7, 10-tetrazacyclododecane is a representative macrocyclic polyamines compound and shows high binding affinity with transition metal ions, such as Zn, Cu and Co. Their metal complexes especially multinuclear complexes were widely used in DNA, RNA recognition.

大环多胺化合物是一类性质独特的优秀配体，具有良好的金属配位能力，其金属配合物作为人工酶模型广泛应用于核酸结构及功能研究、基因表达与调控、抗癌药物的研制等生物、化学和医学领域。1，4，7，10-四氮杂十二烷是典型的大环多胺化合物，对过渡金属离子Zn， Cu and Co有良好的配位能力，这些金属配合物特别是多核金属配合物被广泛应用于DNA识别中。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中，我们发现，除非过量，否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象；过量的La〓（La〓/CaM摩尔比＞4）将引起钙调蛋白结合功能的下降，同时明显改变金属-钙调蛋白-Mas三元复合物的构象；La〓对Ca〓CaM-Mas的N末端表现高度选择性，显示了在三元复合物中两种离子间的协同效应；在三元体系中，La〓与钙调蛋白的亲和力明显上升，而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异，显示了作用的选择性；Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序，La〓很可能优先与C末端结合，从而使Mas/Mas X首先与C末端结合，该顺序与钙离子相同；La〓的参与使三元复合物在动力学上更加稳定。

Affinity chromatography materials for kinase;2. To search a suitable process route of purification of kinase in yeast by affinity chromatography, hexokinase was preparatorily purified by salt preciptation.

为确定一条采用亲和层析技术分离纯化酵母中磷酸激酶的较佳工艺路线，采用分步盐析初步纯化己糖激酶，考察硫酸铵饱争度对盐析效果的影响；以ATP为配基，SESA活化纸纤堆素制备亲和层析剂，确定最佳偶联条件和最佳静态吸附pH值后，将粗酶液直接上亲和层析柱，看分离纯化的效果；在载体和配基之问接上1。

The abundance and occurrence state of an element shows its difference in affinity and could provide important information for the geochemistry system and for the behaviors of an element in the system. The occurrences states of Pt and Pd in the parental rocks and in the fluid inclusions of the Western Guizhou Province basalt are studied. The result of this study shows the importance of determing the occurrence state of an element using its affinity, revealing that the PGE can be used in studying the migration and enrichment of elements in syngenetic fluid of the basalts.

对黔西基性玄武岩原岩与流体包体中Pt、Pd的赋存状态进行研究，结果说明了利用元素亲合性对指导其赋存状态查定的重要性，同时表明了研究该地区玄武岩中同生流体包体中铂族元素对重演古流体中元素迁移与富集具有重要的理论与实际意义。

In this study, nonionic surfactant sorbitan trioleate (Span 85) was modified with Cibacron Blue F-3GA to become an affinity surfactant (CB-Span 85) and to form affinity-based reversed micelles while dissolved in hexane. Since the CB molecule possesses a specific conformation that mimics nicotinamide adenine dinucleotide, it can bind strongly and specifically to a wide range of nucleotide-dependent enzymes such as dehydrogenases and kinases. Moreover, high protein transfer efficiency in the reverse micelles can be easily achieved by controlling the ionic strength of the aqueous phase.

本实验使用非离子型界面活性剂Span 85与蓝色染料分子Cibacron Blue F-3GA结合，形成具有亲和性的界面活性剂 CB-Span 85，由於CB染料分子具有与NAD+相似的结构，可与含核苷酸部位的蛋白质具有亲和力，将CB-Span 85溶於有机溶剂形成反微胞相，可有效的提高反微胞的选择率，可以只控制离子强度来调节蛋白质进出反微胞的效率。

Methods Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.

用亲和层析方法得到的血浆纤维溶酶原经胰蛋白酶消化、层析柱亲和层析、透析，得到纯化的血管抑素；以LA795细胞接种于T739小鼠；接种后第15日起腹腔内注射血管抑素共20 d，观察动物行为，测量荷瘤鼠肿块直径，记录生存期，另设未治疗对照组及观察血管抑素副作用对照组，行肺、肝、肾脏病理学检查。

Based on the retention and Z (the number of solvent molecules involving in t he displacement from stationary-phase surface ), log I (a constant relating t o the affinity of 1 mol of solute to the bonded stationary phase ), and j (a const ant r elating to the affinity of 1 mol of solvent to the bonded stationary phase ), th e elution strength of acetic acid-water mobile phase was found to be stronger th a n that of methanol-water-TFA.

用参数Z （1 mol溶剂化溶质被溶剂化固定相吸附时从两者接触表面释放出置换剂的摩尔总数），log I （与1 mol溶质对固定相亲和势有关的常数）和j（与1 mol溶剂对固定相亲和势有关的常数）对9种蛋白质在此流动相体系中的保留进行了表征。

The McAb against dimethoate showed certain cross reactivity for omethoate and weak cross reactivity for other several analogous compounds, and it displayed low titers and a low affinity constant. The titers of McAb in cells supernatant and ascites were 1:32 and 1:1024, respectively. The affinity constant of McAb was 1.4×10^4 L/mol.

通过杂交瘤技术，获得1株特异分泌抗乐果McAb的杂交瘤细胞，其培养上清与腹水效价分别为1：32和l：1024， McAb亲和常数为1.4×10^4L/mol，与氧化乐果的交叉反应率为10.6%，与其他所测结构类似的农药交叉反应率均小于0.5%。

For the high affinity sites, binding constant decreases with the decrease of the alkyl chain lengh in the surfactants, but for the low affinity sites, binding constant increases and are not distinct when the alkyl chain is dedocyl. The binding sites are related to the alkyl chain lengh, not for the number of hydrophile groups and temperature.Isothermal titration calorimetry has been used to observe the enzyme-catalyzed hydrolysis reaction of urea.

对于高结合位点，结合常数随疏水碳链长度的减小而减小；对于低结合位点，结合常数随疏水碳链长度的减小而增大，相应的当碳链减小至C12时，低结合位点不明显，结合位点数可能只与碳链长度有关，随亲水头基和温度变化不明显。

Competitive indirect enzyme-linked immunosorbent assay was employed to perform the affinity measurement of dissociation constant and affinity constants for bovine milk lactoferrin and IgY specific against LF using antisera from rabbit and hen as references.

以竞争性ELISA法来测定牛乳乳铁蛋白抗原与蛋黄中抗LF特异性抗体间之解离常数与亲合常数，而同时以兔与鸡之抗血清为对照组。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。